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RNH1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3905493

Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to… More details

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Product Overview

Online Datasheet in PDF
Product Name
RNH1, Polyclonal Antibody
Full Product Name
Rabbit anti-RNH1 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
placental ribonuclease inhibitor; placental RNase inhibitor; RAI; ribonuclease inhibitor; Ribonuclease/angiogenin inhibitor 1; RNH; testicular tissue protein Li 164; Ribonuclease inhibitor; ribonuclease/angiogenin inhibitor 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
49,973 Da
OMIM
173320
3D Structure
ModBase 3D Structure for P13489
Predicted Reactivity
Chimpanzee
Immunogen
Between 1 and 50
Conjugation
Unconjugated

Product Categories/Family for anti-RNH1 antibody

Applications Tested/Suitable for anti-RNH1 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-RNH1 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-RNH1 antibody

Detection of human RNH1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RNH1 antibody MBS3905493 (lot MBS3905493-1) used for IP at 6 ug per reaction. RNH1 was also immunoprecipitated by rabbit anti-RNH1 antibody MBS3905492. For blotting immunoprecipitated RNH1, MBS3905493 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-RNH1 Polyclonal Antibody – Cat# MBS3905493

Western Blot (WB) of anti-RNH1 antibody

Detection of human and mouse RNH1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RNH1 antibody MBS3905493 (lot MBS3905493-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-RNH1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3905493

Other Notes

Small volumes of anti-RNH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RNH1 purchase

MBS3905493 is an antibody targeting RNH1. Also known as Rabbit anti-RNH1 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RNH1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ribonuclease inhibitor
NCBI Official Synonym Full Names
ribonuclease/angiogenin inhibitor 1
NCBI Official Symbol
RNH1
NCBI Official Synonym Symbols
RAI; RNH
UniProt Protein Name
Ribonuclease inhibitor
UniProt Synonym Protein Names
Placental ribonuclease inhibitor; Placental RNase inhibitor; Ribonuclease/angiogenin inhibitor 1
Protein Family
UniProt Gene Name
RNH1
UniProt Synonym Gene Names
PRI; RNH; Placental RNase inhibitor; RAI
UniProt Entry Name
RINI_HUMAN
NCBI Protein Information
ribonuclease inhibitor

NCBI Summary for RNH1

Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.[supplied by OMIM, Jul 2010]
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UniProt Comments for RNH1

RNH1: Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play a role in redox homeostasis.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: angiogenin-PRI complex; cytoplasm

Molecular Function: protein binding; ribonuclease inhibitor activity

Biological Process: negative regulation of catalytic activity; mRNA catabolic process; regulation of angiogenesis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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