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Pancreatic Polypeptide/PPY (PPY) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420040

MBS420040 is an antibody targeting PPY. Also known as Goat anti-Pancreatic Polypeptide/PPY Antibody. Reactive in Tested: Human, RatExpected from sequence similarity: Human, Mouse, Rat, Dog. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Pancreatic Polypeptide/PPY, Polyclonal Antibody
Full Product Name
Goat anti-Pancreatic Polypeptide/PPY Antibody
Product Gene Name
anti-PPY antibody
Product Synonym Names
PPY; pancreatic polypeptide; HGNC: 9327; PNP; pancreatic polypeptide Y
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Pancreatic Polypeptide / PPY peptide (MBS426352) is used for blocking the activity of Pancreatic Polypeptide/PPY antibody (MBS420040)
OMIM
167780
Immunogen
Peptide with sequence C-TRPRYGKRHKEDT, from the internal region of the protein sequence according to NP_002713.1.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-PPY antibody
Peptide ELISA, Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PPY antibody
ELISA: Antibody detection limit dilution 1:32000.
WB: Preliminary experiments gave an approx 35-38kDa band in Human Pancreas lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.5kDa according to NP_002713.1. The 38kDa band was successfully blocked by incubation with the immunizing peptide. This antibody has been successfully used in WB on Human.
IHC: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining of rare cells located at the periphery of islets of Langerhans. Recommended concentration: 3µg/ml. Paraffin embedded Human Pancreas and Intestine. Recommended concentration: 5ug/ml. This antibody has been successfully used in IHC on Human.
IF: This antibody has been successfully used in IF on Human and on Rat.

Immunohistochemistry (IHC) of anti-PPY antibody

MBS420040 (3ug/ml) staining of paraffin embedded human pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Goat Anti-PPY Polyclonal Antibody – Cat# MBS420040

Immunohistochemistry (IHC) of anti-PPY antibody

MBS420040 (5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Goat Anti-PPY Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS420040

Immunohistochemistry (IHC) of anti-PPY antibody

MBS420040 (5ug/ml) staining of paraffin embedded Human Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Goat Anti-PPY Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS420040

Other Notes

Small volumes of anti-PPY antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PPY. It may not necessarily be applicable to this product.

NCBI GI #
4506033
NCBI GeneID
5539
NCBI Accession #
NP_002713.1
UniProt Related Accession #
P01298
GenBank Nucleotide #
NM_002722.4
NCBI Official Full Name
pancreatic prohormone isoform 1 preproprotein
NCBI Official Synonym Full Names
pancreatic polypeptide
NCBI Official Symbol
PPY
NCBI Official Synonym Symbols
PP; PNP
UniProt Protein Name
Pancreatic prohormone
UniProt Synonym Protein Names
Pancreatic polypeptide; PP
Protein Family
Pancreatic hormone
UniProt Gene Name
PPY
UniProt Synonym Gene Names
PNP; PP; PH; PI
UniProt Entry Name
PAHO_HUMAN
NCBI Protein Information
pancreatic prohormone

NCBI Summary for PPY

This gene encodes a member of the neuropeptide Y (NPY) family of peptides. The encoded 95 aa preproprotein is synthesized in the pancreatic islets of Langerhans and proteolytically processed to generate two peptide products. These products include the active pancreatic hormone of 36 aa and an icosapeptide of unknown function. This hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]
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UniProt Comments for PPY

PPY: Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions. Belongs to the NPY family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: cytoplasm; extracellular region; extracellular space

Molecular Function: G-protein-coupled receptor binding; hormone activity; neuropeptide hormone activity; receptor binding

Biological Process: cell-cell signaling; digestion; feeding behavior; neuropeptide signaling pathway; positive regulation of defense response to virus by host; protein secretion; regulation of appetite
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Research Articles on PPY

1. Immunohistochemical distribution of this peptide in the epidermal skin (from abdomen, breast and face) of healthy women was analysed.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PPY antibody

 Reference Product  PubMed Publications
 SST antibody  >5 publications with PPY and SST
 GHRL antibody  >3 publications with PPY and GHRL
 NPY2R antibody  >2 publications with PPY and NPY2R
 GAL antibody  >2 publications with PPY and GAL
 NPY5R antibody  >1 publications with PPY and NPY5R
 NPY1R antibody  >1 publications with PPY and NPY1R

Pathways associated with anti-PPY antibody

 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram

Diseases associated with anti-PPY antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >14 publications with PPY and Cardiovascular Diseases
 Inflammation Antibodies  >14 publications with PPY and Inflammation
 Necrosis Antibodies  >12 publications with PPY and Necrosis
 Diabetes Mellitus, Type 2 Antibodies  >8 publications with PPY and Diabetes Mellitus, Type 2
 Disease Models, Animal Antibodies  >7 publications with PPY and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >5 publications with PPY and Neoplasms, Experimental
 Liver Diseases Antibodies  >4 publications with PPY and Liver Diseases
 Diabetes Mellitus, Type 1 Antibodies  >3 publications with PPY and Diabetes Mellitus, Type 1
 Hemorrhage Antibodies  >3 publications with PPY and Hemorrhage
 Hyperplasia Antibodies  >3 publications with PPY and Hyperplasia

Organs/Tissues associated with anti-PPY antibody

 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >17 publications with PPY and Pancreas
 Connective Tissue Antibodies  >13 publications with PPY and Connective Tissue
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