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KLF12 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4381772

Activator protein-2 alpha (AP-2 alpha) is a developmentally-regulated transcription factor and important regulator of gene expression during vertebrate development and carcinogenesis. The protein encoded by this gene is a member of the Kruppel-like zinc… More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
KLF12, Monoclonal Antibody
Full Product Name
KLF12 Mouse Monoclonal Antibody [Clone PCRP-KLF12-1E3]
Product Gene Name
Product Synonym Names
AP-2rep, AP2REP, HSPC122
Clonality
Monoclonal
Isotype
Mouse/IgG1
Clone Number
PCRP-KLF12-1E3
Host
Mouse
Species Reactivity
Human
Predicted to react with Mouse and Rat.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
Purified Ab with BSA and Azide at 200ug/ml
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (lot specific)
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
402
OMIM
607531
3D Structure
ModBase 3D Structure for Q9Y4X4
Immunogen
Recombinant full-length human KLF12 protein
Cellular Localization
Nucleus
Positive Control
HeLa or 293T cells. Human lymph node, spleen or colon.
Hu-chromosome Location
13q22.1
Unigene
729350; 373857
Applications Tested/Suitable for anti-KLF12 antibody
Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-KLF12 antibody
IP: 1-2ug per 100-500ug of total protein)
FC/FACS: 1-2ug/million cells
IF: 1-2ug/ml

SDS-Page of anti-KLF12 antibody

SDS-PAGE Analysis Purified KLF12 Mouse Monoclonal Antibody (PCRP-KLF12-1E3). Confirmation of Purity and Integrity of Antibody.
Mouse Anti-KLF12 Monoclonal Antibody – Cat# MBS4381772

Immunofluorescence (IF) of anti-KLF12 antibody

Mouse Anti-KLF12 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS4381772

Other Notes

Small volumes of anti-KLF12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KLF12 purchase

MBS4381772 is an antibody targeting KLF12. Also known as KLF12 Mouse Monoclonal Antibody [Clone PCRP-KLF12-1E3]. Reactive in Human
Predicted to react with Mouse and Rat.. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for KLF12. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Krueppel-like factor 12
NCBI Official Synonym Full Names
Kruppel-like factor 12
NCBI Official Symbol
KLF12
NCBI Official Synonym Symbols
AP2REP; AP-2rep; HSPC122
UniProt Protein Name
Krueppel-like factor 12
UniProt Synonym Protein Names
Transcriptional repressor AP-2rep
Protein Family
UniProt Gene Name
KLF12
UniProt Synonym Gene Names
AP2REP
UniProt Entry Name
KLF12_HUMAN
NCBI Protein Information
Krueppel-like factor 12; AP-2 repressor; AP-2rep transcription factor; transcriptional repressor AP-2rep; KLF12 zinc finger transcriptional repressor

UniProt Comments for KLF12

KLF12: Confers strong transcriptional repression to the AP-2- alpha gene. Binds to a regulatory element (A32) in the AP-2-alpha gene promoter. Belongs to the Sp1 C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; C2H2-type zinc finger protein; DNA-binding

Chromosomal Location of Human Ortholog: 13q22

Cellular Component: nucleus

Molecular Function: DNA binding; metal ion binding; transcription corepressor activity; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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