Apolipoprotein B (APOB) ELISA Kit for Mouse

Name: Mouse APOB ELISA Kit; Apolipoprotein B; MyBioSource
Brand: MyBioSource
SKU: MBS457703
Price: 550 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS457703

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to APOB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to APOB.… More details

$550 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Apolipoprotein B (APOB), ELISA Kit

Full Product Name

Mouse Apolipoprotein B (APOB) ELISA Kit

Product Gene Name

APOB elisa kit

Product Synonym Names

Apo-B; FLDB; Apo B-100; Apo B-48

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, Plasma or Other Biological Fluids.

Detection Range

3.12-200ng/mL

Sensitivity

1.22ng/mL

Preparation and Storage

For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Molecular Weight

509,432 Da

3D Structure

ModBase 3D Structure for E9Q414

RTU ELISA Kit's Advantage

The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of APOB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for APOB purchase

MBS457703 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Apolipoprotein B (APOB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing APOB. The ELISA analytical biochemical technique of the MBS457703 kit is based on APOB antibody-APOB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect APOB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, APOB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for APOB. It may not necessarily be applicable to this product.

NCBI GI #

161702988

NCBI GeneID

238055

NCBI Accession #

NP_033823.2

UniProt Primary Accession #

E9Q414

UniProt Secondary Accession #

Q3UH74; Q497D8; Q61314; Q61318; Q8CGG8

UniProt Related Accession #

E9Q414

GenBank Nucleotide #

NM_009693.2

NCBI Official Full Name

apolipoprotein B-100

NCBI Official Synonym Full Names

apolipoprotein B

NCBI Official Symbol

Apob

NCBI Official Synonym Symbols

apob-48; AI315052; apob-100; Apo B-100

UniProt Protein Name

Apolipoprotein B-100

Protein Family

Apolipoprotein

UniProt Gene Name

Apob

UniProt Synonym Gene Names

Apo B-100; Apo B-48

NCBI Protein Information

apolipoprotein B-100

NCBI Summary for APOB

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100. Unlike the apoB-48 and apoB-100 structural equivalents in human, which are synthesized exclusively in the gut and liver, respectively, the mouse apoB-48 isoform is also found in mouse liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2179 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. [provided by RefSeq, Jul 2008]

UniProt Comments for APOB

Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

Research Articles on APOB

1. Atorvastatin therapy did not show cholesterol-independent effects on inflammation in atherosclerotic lesions in Ldlr(-/-)Apob(100/100) mice, whereas a cholesterol-lowering diet intervention was effective.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with APOB elisa kit

Reference Product	PubMed Publications
Apoe elisa kit	>1561 publications with APOB and Apoe
Apoa1 elisa kit	>584 publications with APOB and Apoa1
Ldlr elisa kit	>532 publications with APOB and Ldlr
Lpl elisa kit	>251 publications with APOB and Lpl
Mttp elisa kit	>65 publications with APOB and Mttp
Apoc3 elisa kit	>65 publications with APOB and Apoc3
Apoa2 elisa kit	>24 publications with APOB and Apoa2
Plg elisa kit	>4 publications with APOB and Plg

Pathways associated with APOB elisa kit

Products by Pathway	Pathway Diagram
Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies	Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
Cargo Recognition For Clathrin-mediated Endocytosis Pathway antibodies	Cargo Recognition For Clathrin-mediated Endocytosis Pathway Diagram
Cell Surface Interactions At The Vascular Wall Pathway antibodies	Cell Surface Interactions At The Vascular Wall Pathway Diagram
Chylomicron-mediated Lipid Transport Pathway antibodies	Chylomicron-mediated Lipid Transport Pathway Diagram
Clathrin-mediated Endocytosis Pathway antibodies	Clathrin-mediated Endocytosis Pathway Diagram
Fat Digestion And Absorption Pathway antibodies	Fat Digestion And Absorption Pathway Diagram
Fat Digestion And Absorption Pathway antibodies	Fat Digestion And Absorption Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram

Diseases associated with APOB elisa kit

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>3782 publications with APOB and Cardiovascular Diseases
Dyslipidemias Antibodies	>3249 publications with APOB and Dyslipidemias
Atherosclerosis Antibodies	>2793 publications with APOB and Atherosclerosis
Hyperlipidemias Antibodies	>2614 publications with APOB and Hyperlipidemias
Coronary Disease Antibodies	>1991 publications with APOB and Coronary Disease
Heart Diseases Antibodies	>1743 publications with APOB and Heart Diseases
Hypercholesterolemia Antibodies	>1618 publications with APOB and Hypercholesterolemia
Insulin Resistance Antibodies	>814 publications with APOB and Insulin Resistance
Hyperlipoproteinemia Type II Antibodies	>775 publications with APOB and Hyperlipoproteinemia Type II
Hypertriglyceridemia Antibodies	>667 publications with APOB and Hypertriglyceridemia

Organs/Tissues associated with APOB elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>2419 publications with APOB and Liver
Intestine Antibodies	>635 publications with APOB and Intestine
Kidney Antibodies	>461 publications with APOB and Kidney
Muscle Antibodies	>287 publications with APOB and Muscle
Lung Antibodies	>156 publications with APOB and Lung
Embryonic Tissue Antibodies	>61 publications with APOB and Embryonic Tissue
Spleen Antibodies	>59 publications with APOB and Spleen
Bone Marrow Antibodies	>39 publications with APOB and Bone Marrow
Lymph Node Antibodies	>15 publications with APOB and Lymph Node
Extraembryonic Tissue Antibodies	>1 publications with APOB and Extraembryonic Tissue