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Phosphotyrosine Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS472051

Phosphorylation of specific tyrosine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor tyrosine kinases that… More details

$435 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Phosphotyrosine, Polyclonal Antibody
Full Product Name
Anti-Phosphotyrosine
Product Synonym Names
Phosphotyrosine; Ptyr
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Rat, Mouse, Chicken
Form/Format
Liquid PBS +1 mg/ml, 0.05% NaN3 and 50% glycerol.
Purity/Purification
Antigen Affinity Purified
Specificity
This antibody detects phosphotyrosine-containing proteins on SDS-PAGE immunoblots of EGF treated A431 cells, as well as pervanadate treated A431, HeLa, Jurkat, and endothelial cells.
Preparation and Storage
Store at -20°C. As aliquots may be taken without freeze/thawing due to presence of 50% glycerol.
Immunogen
Anti-Phosphotyrosine was generated from a panel of phosphotyrosine-containing peptide immunogens designed from human protein sequences. All peptide sequences used are highly conserved phosphotyrosine motifs.
Applications Tested/Suitable for anti-Phosphotyrosine antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes for anti-Phosphotyrosine antibody
WB: 1:1000
ICC: 1:100
IP: 1:100

Testing Data of anti-Phosphotyrosine antibody

Immunocytochemical labeling of phosphotyrosine in control and pervanadate-treated A431 cells. The cells were labeled with rabbit polyclonal anti-Phosphotyrosine and mouse monoclonal anti-Phosphotyrosine , then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
Rabbit Anti-Phosphotyrosine Polyclonal Antibody – Cat# MBS472051

Testing Data of anti-Phosphotyrosine antibody

Bar graph showing rabbit polyclonal anti-Phosphotyrosine binding to a variety of phosphotyrosine containing peptides, but no binding to unphosphorylated peptide (beta-Catenin (a.a. 29-45).
Rabbit Anti-Phosphotyrosine Polyclonal Antibody, Testing Data – Cat# MBS472051

Product References and Citations for anti-Phosphotyrosine antibody

• Ross, A.H. et al. (1981) Nature 294:654.
• Wang, J.Y.J. (1988) Anal. Biochem 172:1.

Other Notes

Small volumes of anti-Phosphotyrosine antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Phosphotyrosine purchase

MBS472051 is an antibody targeting Phosphotyrosine. Also known as Anti-Phosphotyrosine. Reactive in Human, Rat, Mouse, Chicken. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Phosphotyrosine. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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