Rabbit Anti-PFN1 Polyclonal Antibody – Cat# MBS474133

Western blot of HUVEC stimulated with Pervanadate (1 mM) for 30 min (lanes 1-4). The blots were treated with alkaline phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Profilin (a.a. 126-137) (lanes 1 & 2) or anti-Profilin (Tyr-129) phospho-specific (lanes 3 & 4). The antibodies detect profilin at 15 kDa. In lanes 3 & 4, the antibody also detects an unknown 20 kDa protein.

Profilin (PFN1) Antibody – Rabbit Polyclonal

Name: PFN1 Antibody; Polyclonal; Rabbit; Human/Rat/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS474133
Price: 435 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS474133

Profilins are small actin-binding proteins that have functions in cell motility, cytokinesis, gene transcription, endocytosis and neuronal plasticity. Four profilin isoforms have been identified in mammals. Profilin-1 (PFN1) and profilin-2a (PFN2a) isoforms… More details

$435 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Profilin (PFN1), Polyclonal Antibody

Full Product Name

Profilin (a.a. 126-137)

Product Gene Name

anti-PFN1 antibody

Product Synonym Names

Epididymis Li184a; PFN1; PFN2

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Rat, Mouse

Form/Format

Supplied in 100ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Purity/Purification

Affinity Purified

Specificity

This antibody was affinity purified using Profilin (a.a. 126-137) peptide (without carrier). The antibody detects a 15 kDa band corresponding to the apparent molecular mass of Profilin-1 in human umbilical vein endothelial cells (HUVEC) and Jurkat cells.

Preparation and Storage

Store at -20 degree C. Stable for 1 year.

Molecular Weight

15 kDa

OMIM

176610

3D Structure

ModBase 3D Structure for P07737

Immunogen

Profilin synthetic peptide (coupled to carrier protein) that includes amino acids 126 to 137 in human Profilin-1. This sequence is well conserved in rat and mouse Profilin-1, has some homology to Profilin-2a and Profilin-2b, and low homology to Profiin-3 and Profilin-4.

Applications Tested/Suitable for anti-PFN1 antibody

Western Blot (WB), ELISA (EIA)

Application Notes for anti-PFN1 antibody

ELISA: 1:2000
WB: 1:1000
WB: Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.

Product References and Citations for anti-PFN1 antibody

Shao, J. et al. (2008) Mol Cell Biol. 28(17):5196.Fan, Y. et al. (2012) Nat Cell Biol. 14(10):1046.Shao, J. & Diamond, M.I. (2012) PLoS ONE 7(3): e32802.

Other Notes

Small volumes of anti-PFN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PFN1 purchase

MBS474133 is an antibody targeting PFN1. Also known as Profilin (a.a. 126-137). Reactive in Human, Rat, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PFN1. It may not necessarily be applicable to this product.

NCBI GI #

4826898

NCBI GeneID

5216

NCBI Accession #

NP_005013.1

UniProt Primary Accession #

P07737

UniProt Related Accession #

P07737

GenBank Nucleotide #

NM_005022.3

NCBI Official Full Name

profilin-1

NCBI Official Synonym Full Names

profilin 1

NCBI Official Symbol

PFN1

NCBI Official Synonym Symbols

ALS18

UniProt Protein Name

Profilin-1

UniProt Synonym Protein Names

Epididymis tissue protein Li 184a; Profilin I

Protein Family

Profilin

UniProt Gene Name

PFN1

UniProt Entry Name

PROF1_HUMAN

NCBI Protein Information

profilin-1

NCBI Summary for PFN1

This gene encodes a member of the profilin family of small actin-binding proteins. The encoded protein plays an important role in actin dynamics by regulating actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome, and the encoded protein may also play a role in Huntington disease. Multiple pseudogenes of this gene are located on chromosome 1. [provided by RefSeq, Jul 2012]

Research Articles on PFN1

1. mutation-induced stabilization of a partially folded state can enhance profilin-1 aggregation and thereby contribute to the pathogenicity of the mutations

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PFN1 antibody

Reference Product	PubMed Publications
VASP antibody	>24 publications with PFN1 and VASP
WASL antibody	>4 publications with PFN1 and WASL
CAP1 antibody	>2 publications with PFN1 and CAP1
CFL1 antibody	>2 publications with PFN1 and CFL1
ACTG1 antibody	>1 publications with PFN1 and ACTG1
APBB1IP antibody	>1 publications with PFN1 and APBB1IP

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Diseases associated with anti-PFN1 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>28 publications with PFN1 and Neoplasms
Breast Neoplasms Antibodies	>12 publications with PFN1 and Breast Neoplasms
Adenocarcinoma Antibodies	>9 publications with PFN1 and Adenocarcinoma
Nervous System Diseases Antibodies	>8 publications with PFN1 and Nervous System Diseases
Kidney Diseases Antibodies	>5 publications with PFN1 and Kidney Diseases
Liver Neoplasms Antibodies	>4 publications with PFN1 and Liver Neoplasms
Inflammation Antibodies	>4 publications with PFN1 and Inflammation
Liver Diseases Antibodies	>4 publications with PFN1 and Liver Diseases
Carcinoma, Hepatocellular Antibodies	>4 publications with PFN1 and Carcinoma, Hepatocellular
Brain Diseases Antibodies	>4 publications with PFN1 and Brain Diseases

Organs/Tissues associated with anti-PFN1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>37 publications with PFN1 and Blood
Brain Antibodies	>30 publications with PFN1 and Brain
Muscle Antibodies	>21 publications with PFN1 and Muscle
Vascular Antibodies	>15 publications with PFN1 and Vascular
Kidney Antibodies	>13 publications with PFN1 and Kidney
Nerve Antibodies	>12 publications with PFN1 and Nerve
Embryonic Tissue Antibodies	>10 publications with PFN1 and Embryonic Tissue
Bone Antibodies	>8 publications with PFN1 and Bone
Liver Antibodies	>7 publications with PFN1 and Liver
Bone Marrow Antibodies	>7 publications with PFN1 and Bone Marrow