Rabbit Anti-SYN1 Polyclonal Antibody – Cat# MBS502214

Synapsin I (100 Ul) (SYN1) Antibody – Rabbit Polyclonal

Name: SYN1 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS502214
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502214

MBS502214 is an antibody targeting SYN1. Also known as Anti-Synapsin I. Reactive in Human, mouse, rat. Host: Rabbit. Affinity purified rabbit polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Synapsin I (100 ul) (SYN1), Polyclonal Antibody

Full Product Name

Anti-Synapsin I

Product Gene Name

anti-SYN1 antibody

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, mouse, rat

Form/Format

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Purity/Purification

Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the native protein was coupled.)

Specificity

Specific for the ~78k synapsin I doublet in Western blots of rat brain extracts. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody.

Preparation and Storage

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year

Molecular Weight

Chromosome Location

Chromosome: X; AC_000187.1 (91192243..91229595, complement). Location: chromosome: X

3D Structure

ModBase 3D Structure for P17599

Antigen

Native protein purified from bovine brain.

Immunogen Information

Native protein purified from bovine brain

Immunogen Species

Bovine

Species Reactivity Note

The antibody has been directly tested for reactivity in Western blots with rat, mouse and human tissue.

Biological Significance

Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002).

Applications Tested/Suitable for anti-SYN1 antibody

Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Application Notes for anti-SYN1 antibody

Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC: 1:1000
IF: 1:1000
IP: 2 ul per 200 ug lysate

Product References and Citations for anti-SYN1 antibody

• Feng J, Chi P, Blanpied TA, Xu YM, Magarinos AM, Ferreira A, Takahashi RH, Kao HT, McEwen BS, Ryan TA, Augustine GJ, Greengard P (2002) Regulation of neurotransmitter release by synapsin III. J Neurosci 22:4372-4380.
• Jovanovic JN, Sihra TS, Nairn AC, Hemmings HC, Jr., Greengard P, Czernik AJ (2001) Opposing changes in phosphorylation of specific sites in synapsin I during Ca2+-dependent glutamate release in isolated nerve terminals. J Neurosci 21:7944-7953.
• Kao HT, Song HJ, Porton B, Ming GL, Hoh J, Abraham M, Czernik AJ, Pieribone VA, Poo MM, Greengard P (2002) A protein kinase A-dependent molecular switch in synapsins regulates neurite outgrowth. Nature Neurosci 5:431-437.
• Moore RY, Bernstein M (1989) Synaptogenesis in the rat suprachiasmatic nucleus demonstrated by electron microscopy and synapsin I immunoreactivity. J Neurosci 9:2151-2162.
• Nayak AS, Moore CI, Browning MD (1996) CaM kinase II phosphorylation of the presynaptic protein synapsin is persistently increased during expression of long-term potentiation. Proc Natl Acad Sci (USA) 93:15451-15456.

Other Notes

Small volumes of anti-SYN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SYN1. It may not necessarily be applicable to this product.

NCBI GI #

27806393

NCBI GeneID

281510

NCBI Accession #

NP_776616.1

UniProt Primary Accession #

P17599

UniProt Related Accession #

P17599

GenBank Nucleotide #

NM_174191.2

NCBI Official Full Name

synapsin-1

NCBI Official Symbol

SYN1

UniProt Protein Name

Synapsin-1

UniProt Synonym Protein Names

Synapsin I

Protein Family

Sister chromatid cohesion 1 protein

UniProt Gene Name

SYN1

UniProt Entry Name

SYN1_BOVIN

NCBI Protein Information

synapsin-1

UniProt Comments for SYN1

Function: Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level

By similarity.

Subunit structure: Homodimer. Interacts with CAPON. Forms a ternary complex with NOS1. Isoform Ibinteracts with PRNP

By similarity.

Subcellular location: Cell junction › synapse. Golgi apparatus

By similarity.

Domain: The A region binds phospholipids with a preference for negatively charged species

By similarity.

Post-translational modification: Substrate of at least four different protein kinases. Phosphorylation may play a role in the regulation of synapsin-1 in the nerve terminal.Phosphorylation at Ser-9 dissociates synapsins from synaptic vesicles

By similarity.

Sequence similarities: Belongs to the synapsin family.

Research Articles on SYN1

1. These results raise the possibility that Ser(603) on synapsin I is alternatively phosphorylated by p21-activated kinases, not only by CaMKII, in neuronal cells in response to some stimulants.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-SYN1 antibody

Disease Name	Pubmed Publications
Brain Diseases Antibodies	>11 publications with SYN1 and Brain Diseases
Mental Disorders Antibodies	>11 publications with SYN1 and Mental Disorders
Central Nervous System Diseases Antibodies	>9 publications with SYN1 and Central Nervous System Diseases
Neoplasms Antibodies	>6 publications with SYN1 and Neoplasms
Cognition Disorders Antibodies	>4 publications with SYN1 and Cognition Disorders
Neurobehavioral Manifestations Antibodies	>4 publications with SYN1 and Neurobehavioral Manifestations
Seizures Antibodies	>3 publications with SYN1 and Seizures
Learning Disorders Antibodies	>3 publications with SYN1 and Learning Disorders
Chromosome Aberrations Antibodies	>3 publications with SYN1 and Chromosome Aberrations
Necrosis Antibodies	>3 publications with SYN1 and Necrosis

Organs/Tissues associated with anti-SYN1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>29 publications with SYN1 and Brain
Kidney Antibodies	>1 publications with SYN1 and Kidney