Mouse Anti-ADAMTS4 Monoclonal Antibody – Cat# MBS608876

ADAMTS4 Antibody – Mouse Monoclonal

Name: ADAMTS4 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS608876
Price: 1275 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Popular Catalog #: MBS608876

ADAMTS4 is a secreted member of the ADAM family of proteins. ADAMTS4 is upregulated by TNF-alpha in osteoarthritis and contributes to joint destruction. Its upregulation in some cancers may enhance tumor invasiveness. Human ADAMTS4 consists of a signal… More details

$1275 / 0.5 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ADAMTS4, Monoclonal Antibody

Full Product Name

ADAMTS4 (Aggrecanase I, A Disintegrin And Metalloproteinase with Thrombospondin motif 4)

Product Gene Name

anti-ADAMTS4 antibody

Product Synonym Names

Anti -ADAMTS4 (Aggrecanase I, A Disintegrin And Metalloproteinase with Thrombospondin motif 4)

Clonality

Monoclonal

Isotype

IgG2a

Clone Number

9H40

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.

Purity/Purification

Affinity Purified
Purified by Protein G affinity chromatography.

Specificity

Recognizes human ADAMTS4. Does not crossreact with recombinant human ADAMTS1, ADAMTS5, ADAMTS13 or ADAMTSL1.2. Species Sequence Homology: chimpanzee; 100%, canine; 97%, mouse, rat, bovine; 94%

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

90,197 Da

Chromosome Location

Chromosome: 1; NC_000001.10 (161159538..161168845, complement). Location: 1q21-q23

OMIM

603876

3D Structure

ModBase 3D Structure for O75173

Immunogen

Recombinant corresponding to aa213-685 from human ADAMTS4 expressed in NSO cells (O75173).

Product Categories/Family for anti-ADAMTS4 antibody

Antibodies; Abs to Matrix Metalloproteinases

Applications Tested/Suitable for anti-ADAMTS4 antibody

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Neutralization

Application Notes for anti-ADAMTS4 antibody

Suitable for use in Neutralization, Direct ELISA, Western Blot and Immunoprecipitation.
Dilution: Neutralization: 25ug/ml (IC50) inhibits 50% of the proteolytic activity under conditions in which the enzyme was present at 5ug/ml and the substrate concentration was 100ug/ml. The antibody was preincubated with the enzyme at different molar ratios at RT for 30 minutes. The enzyme was then assayed with recombinant human Aggrecan G1-IGD-G2 domains as the substrate. Considering the molecular mass of the enzyme (50kD) and the antibody (150kD), IC50 was achieved at ~2:1 molar ratio of the antibody to enzyme.
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~2ng/well.
Western Blot: 1-2ug/ml. Using a colormetric system, the detection limit is ~25ng/lane under non-reducing conditions. Use under reducing conditions is not recommended.
Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human ADAMTS4 from conditioned cell medium.

Other Notes

Small volumes of anti-ADAMTS4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ADAMTS4 purchase

MBS608876 is an antibody targeting ADAMTS4. Also known as ADAMTS4 (Aggrecanase I, A Disintegrin And Metalloproteinase with Thrombospondin motif 4). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ADAMTS4. It may not necessarily be applicable to this product.

NCBI GI #

157427675

NCBI GeneID

9507

NCBI Accession #

NP_005090.3

UniProt Primary Accession #

O75173

UniProt Secondary Accession #

Q5VTW2; Q6P4Q8; Q6UWA8; Q9UN83

UniProt Related Accession #

O75173; Q2HYD0

GenBank Nucleotide #

NM_005099.4

NCBI Official Full Name

A disintegrin and metalloproteinase with thrombospondin motifs 4 preproprotein

NCBI Official Synonym Full Names

ADAM metallopeptidase with thrombospondin type 1 motif, 4

NCBI Official Symbol

ADAMTS4

NCBI Official Synonym Symbols

ADMP-1; ADAMTS-2; ADAMTS-4; KIAA0688

UniProt Protein Name

A disintegrin and metalloproteinase with thrombospondin motifs 4

UniProt Synonym Protein Names

ADMP-1; Aggrecanase-1

Protein Family

A disintegrin and metalloproteinase with thrombospondin motifs

UniProt Gene Name

ADAMTS4

UniProt Synonym Gene Names

KIAA0688

UniProt Entry Name

ATS4_HUMAN

NCBI Protein Information

A disintegrin and metalloproteinase with thrombospondin motifs 4; ADAM-TS4; ADAM-TS 4; aggrecanase-1; OTTHUMP00000032249; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4

NCBI Summary for ADAMTS4

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma. [provided by RefSeq]

UniProt Comments for ADAMTS4

ADAMTS4: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-|-Ala-393' site.

Protein type: EC 3.4.24.82; Secreted; Motility/polarity/chemotaxis; Protease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q21-q23

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: peptidase activity; protein binding; protease binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; defense response to bacterium; proteolysis; skeletal development

Research Articles on ADAMTS4

1. Patients with acute coronary syndrome showed increased ADAMTS4 expression, which may aggravate the development of atherosclerosis and instability of atherosclerotic plaques.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ADAMTS4 antibody

Reference Product	PubMed Publications
BCAN antibody	>3 publications with ADAMTS4 and BCAN
FURIN antibody	>2 publications with ADAMTS4 and FURIN
SRPX2 antibody	>1 publications with ADAMTS4 and SRPX2
ACAN antibody	>1 publications with ADAMTS4 and ACAN

Pathways associated with anti-ADAMTS4 antibody

Products by Pathway	Pathway Diagram
Endochondral Ossification Pathway antibodies	Endochondral Ossification Pathway Diagram

Diseases associated with anti-ADAMTS4 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>9 publications with ADAMTS4 and Inflammation
Arthritis, Rheumatoid Antibodies	>4 publications with ADAMTS4 and Arthritis, Rheumatoid
Pain Antibodies	>4 publications with ADAMTS4 and Pain
Carcinoma Antibodies	>4 publications with ADAMTS4 and Carcinoma
Sarcoma Antibodies	>4 publications with ADAMTS4 and Sarcoma
Atherosclerosis Antibodies	>3 publications with ADAMTS4 and Atherosclerosis
Neoplasm Invasiveness Antibodies	>3 publications with ADAMTS4 and Neoplasm Invasiveness
Brain Neoplasms Antibodies	>3 publications with ADAMTS4 and Brain Neoplasms
Metaplasia Antibodies	>2 publications with ADAMTS4 and Metaplasia
Nervous System Neoplasms Antibodies	>2 publications with ADAMTS4 and Nervous System Neoplasms

Organs/Tissues associated with anti-ADAMTS4 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>11 publications with ADAMTS4 and Brain
Muscle Antibodies	>4 publications with ADAMTS4 and Muscle
Embryonic Tissue Antibodies	>3 publications with ADAMTS4 and Embryonic Tissue
Lung Antibodies	>1 publications with ADAMTS4 and Lung