RPMI 1640 Medium Modified w/L-Glutamine w/o Phenol Red Culture Media

Off white to yellow, homogenous, free flowing powder

Cell Culture Grade

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4 degree C and used within a short period of time.

< 1EU/ml

8.5±0.5

Colorless, clear, complete

Inorganic Salts:
Calcium Nitrate 4H2O 0.1
Magnesium Sulfate 0.04884
Potassium Chloride 0.4
Sodium Chloride 6
Sodium Phosphate Dibasic 0.8

Amino Acids:
L-Arginine 0.2
L-Asparagine 0.05
L-Aspartic Acid 0.02
pL-Cystine 2HCl 0.0652
L-Glutamic Acid 0.02
L-Glutamine 0.3
Glycine 0.01
L-Histidine 0.015
Hydroxy-L-proline 0.02
L-Isoleucine 0.05
L-Leucine 0.05
L-Lysine HCl 0.04
L-Methionine 0.015
L-Phenylalanine 0.015
L-Proline 0.02
L-Serine 0.03
L-Threonine 0.02
L-Tryptophan 0.005
L-Tyrosine 2Na 2H2O 0.02883
L-Valine 0.02

Vitamins:
P-Amino Benzoic Acid 0.001
D-Biotin 0.0002
Choline Chloride 0.003
Vitamin B-12 0.000005
Folic Acid 0.001
myo-Inositol 0.035
Niacinamide 0.001
D-Pantothenic Acid Ca 0.00025
Pyridoxine HCl 0.001
Riboflavin 0.0002
ThiamineHCl 0.001

Other:
D-Glucose 2
Glutathione 0.001

Total: 10.4 g/liter

Dissolve 10.4g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2g sodium bicarbonate while stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Note: It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate.

Hygroscopic. Keep tightly closed and desiccated.

Culture Media; Media-Cell Culture; RPMI