Alpha-2-Hs-Glyco (AHSG) ELISA Kit for Human

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Fetuin A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Fetuin A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Fetuin A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Fetuin A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of AHSG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS701409 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the alpha-2-HS-glycoprotein (AHSG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AHSG. The ELISA analytical biochemical technique of the MBS701409 kit is based on AHSG antibody-AHSG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AHSG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AHSG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Proteases breakdown proteins but must be carefully regulated by endogenous inhibitors like Fetuin A. In a feedback regulatory loop this ABSG fetus glycoprotein is regulated by other factors in bone development. To understand this complex dance, quantitation by Fetuin ELISA is now possible thanks to MyBiosource and LSBio. We provide an excellent Fetuin alpha 2 ELISA kit with optimized antibody pairs, selective secondary antibodies and backed by our promise of value. Whether your assay sample types are cell culture media or human serum plasma, this sandwich format custom glycoprotein ELISA will accurately measure protein. ELISA kits come complete with all reagents, 96 tests and a Fetuin ABSG standard for comparison down to ng ml ranges. When you place your order for catalog number MBS701409, don't forget to check out our mouse Fetuin sandwich ELISA and polyclonal antibody.