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Adenosine A3 Receptor (ADORA3) ELISA Kit for Rat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS764359

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat ADORA3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat ADORA3 antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Adenosine A3 Receptor (ADORA3), ELISA Kit
Full Product Name
Rat Adenosine A3 Receptor ELISA Kit
Product Gene Name
ADORA3 elisa kit
Product Synonym Names
ADORA3
Species Reactivity
Rat
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
38,976 Da
OMIM
600445
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of ADORA3 elisa kit

Rat ADORA3 ELISA Kit – Cat# MBS764359

Typical Standard Curve of ADORA3 elisa kit

Rat ADORA3 ELISA Kit, Typical Standard Curve – Cat# MBS764359

Other Notes

Small volumes of ADORA3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ADORA3 purchase

MBS764359 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Adenosine A3 Receptor (ADORA3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADORA3. The ELISA analytical biochemical technique of the MBS764359 kit is based on ADORA3 antibody-ADORA3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADORA3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADORA3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ADORA3. It may not necessarily be applicable to this product.

NCBI GI #
4262078
NCBI GeneID
140
NCBI Accession #
AAD14378.1
UniProt Secondary Accession #
P33765; Q6UWU0; Q9BYZ1; A2A3P4
UniProt Related Accession #
P0DMS8
NCBI Official Full Name
adenosine A3 receptor, partial
NCBI Official Synonym Full Names
adenosine A3 receptor
NCBI Official Symbol
ADORA3
NCBI Official Synonym Symbols
A3AR
UniProt Protein Name
Adenosine receptor A3
Protein Family
Adenosine receptor
UniProt Gene Name
ADORA3
UniProt Entry Name
AA3R_HUMAN
NCBI Protein Information
adenosine receptor A3

NCBI Summary for ADORA3

This gene encodes a protein that belongs to the family of adenosine receptors, which are G-protein-coupled receptors that are involved in a variety of intracellular signaling pathways and physiological functions. The receptor encoded by this gene mediates a sustained cardioprotective function during cardiac ischemia, it is involved in the inhibition of neutrophil degranulation in neutrophil-mediated tissue injury, it has been implicated in both neuroprotective and neurodegenerative effects, and it may also mediate both cell proliferation and cell death. Alternative splicing results in multiple transcript variants. This gene shares its 5' terminal exon with some transcripts from overlapping GeneID:57413, which encodes an immunoglobulin domain-containing protein. [provided by RefSeq, Nov 2014]
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UniProt Comments for ADORA3

ADORA3: Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. Possible role in reproduction. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: integral to membrane; plasma membrane

Molecular Function: adenosine receptor activity, G-protein coupled

Biological Process: adenosine receptor signaling pathway
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Research Articles on ADORA3

1. This study identifies human mast cell A3R as regulator of tissue remodeling gene expression in human mast cells and demonstrates a heretofore-unrecognized mode of feedback regulation that is exerted by this receptor.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with ADORA3 elisa kit

 Products by Pathway  Pathway Diagram
 Adenosine P1 Receptors Pathway antibodies  Adenosine P1 Receptors Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 GPCRs, Other Pathway antibodies  GPCRs, Other Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Nucleotide GPCRs Pathway antibodies  Nucleotide GPCRs Pathway Diagram

Diseases associated with ADORA3 elisa kit

 Disease Name  Pubmed Publications
 Ischemia Antibodies  >184 publications with ADORA3 and Ischemia
 Cardiovascular Diseases Antibodies  >162 publications with ADORA3 and Cardiovascular Diseases
 Inflammation Antibodies  >155 publications with ADORA3 and Inflammation
 Heart Diseases Antibodies  >108 publications with ADORA3 and Heart Diseases
 Kidney Diseases Antibodies  >103 publications with ADORA3 and Kidney Diseases
 Brain Diseases Antibodies  >91 publications with ADORA3 and Brain Diseases
 Necrosis Antibodies  >75 publications with ADORA3 and Necrosis
 Lung Diseases Antibodies  >49 publications with ADORA3 and Lung Diseases
 Myocardial Infarction Antibodies  >39 publications with ADORA3 and Myocardial Infarction
 Pain Antibodies  >31 publications with ADORA3 and Pain

Organs/Tissues associated with ADORA3 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >250 publications with ADORA3 and Blood
 Brain Antibodies  >226 publications with ADORA3 and Brain
 Heart Antibodies  >194 publications with ADORA3 and Heart
 Kidney Antibodies  >154 publications with ADORA3 and Kidney
 Lung Antibodies  >87 publications with ADORA3 and Lung
 Bone Antibodies  >41 publications with ADORA3 and Bone
 Eye Antibodies  >41 publications with ADORA3 and Eye
 Nerve Antibodies  >41 publications with ADORA3 and Nerve
 Intestine Antibodies  >27 publications with ADORA3 and Intestine
 Testis Antibodies  >22 publications with ADORA3 and Testis
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