Rabbit Anti-MED30 Polyclonal Antibody – Cat# MBS821102

Western blot analysis of MED30 expression in HCT116 (A), HEK293T (B), Raw264.7 (C), H9C2 (D) whole cell lysates.

MED30 Antibody – Rabbit Polyclonal

Name: MED30 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS821102
Price: 225 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS821102

MBS821102 is an antibody targeting MED30. Reactive in Human, Mouse, Rat, Monkey. Host: Rabbit. Rabbit polyclonal antibody to MED30 More details

$225 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MED30, Polyclonal Antibody

Full Product Name

Anti-MED30 Antibody

Product Gene Name

anti-MED30 antibody

Product Synonym Names

THRAP6; TRAP25; Mediator of RNA polymerase II transcription subunit 30; Mediator complex subunit 30; TRAP/Mediator complex component TRAP25; Thyroid hormone receptor-associated protein 6; Thyroid hormone receptor-associated protein complex 25 kDa component; Trap25

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Monkey

Form/Format

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purity/Purification

The antibody was purified by immunogen affinity chromatography.

Specificity

Recognizes endogenous levels of MED30 protein.

Preparation and Storage

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Antibody/Peptide Pairs

MED30 peptide (MBS823803) is used for blocking the activity of MED30 antibody (MBS821102)

Molecular Weight

16,279 Da

OMIM

610237

3D Structure

ModBase 3D Structure for Q96HR3

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MED30. The exact sequence is proprietary.

Applications Tested/Suitable for anti-MED30 antibody

Western Blot (WB)

Application Notes for anti-MED30 antibody

WB (1/500 - 1/1000)

Other Notes

Small volumes of anti-MED30 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MED30. It may not necessarily be applicable to this product.

NCBI GI #

545479145

NCBI GeneID

90390

NCBI Accession #

NP_001269915.1

UniProt Primary Accession #

Q96HR3

UniProt Secondary Accession #

C6GKU9

UniProt Related Accession #

Q96HR3

GenBank Nucleotide #

NM_001282986.1

NCBI Official Full Name

mediator of RNA polymerase II transcription subunit 30 isoform 2

NCBI Official Synonym Full Names

mediator complex subunit 30

NCBI Official Symbol

MED30

NCBI Official Synonym Symbols

MED30S; THRAP6; TRAP25

UniProt Protein Name

Mediator of RNA polymerase II transcription subunit 30

UniProt Synonym Protein Names

Mediator complex subunit 30; TRAP/Mediator complex component TRAP25; Thyroid hormone receptor-associated protein 6; Thyroid hormone receptor-associated protein complex 25 kDa component; Trap25

Protein Family

Mediator of RNA polymerase II transcription

UniProt Gene Name

MED30

UniProt Synonym Gene Names

THRAP6; TRAP25; Trap25

UniProt Entry Name

MED30_HUMAN

NCBI Protein Information

mediator of RNA polymerase II transcription subunit 30; TRAP/Mediator complex component TRAP25; thyroid hormone receptor associated protein 6; thyroid hormone receptor-associated protein 6; putative mediator of RNA polymerase II transcription subunit 30; thyroid hormone receptor-associated protein complex 25 kDa component

NCBI Summary for MED30

The multiprotein TRAP/Mediator complex facilitates gene expression through a wide variety of transcriptional activators. MED30 is a component of this complex that appears to be metazoan specific (Baek et al., 2002 [PubMed 11909976]).[supplied by OMIM, Nov 2010]

UniProt Comments for MED30

MED30: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 30 family.

Protein type: Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 8q24.11

Cellular Component: nucleoplasm; Srb-mediator complex; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor transcription coactivator activity; vitamin D receptor binding; transcription cofactor activity; thyroid hormone receptor binding; receptor activity

Biological Process: steroid hormone receptor signaling pathway; regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; stem cell maintenance; gene expression

Research Articles on MED30

1. analysis of a novel transcript of MED30, termed MED30 short (MED30s) generated by alternative splicing

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MED30 antibody

Reference Product	PubMed Publications
MED12 antibody	>3 publications with MED30 and MED12
MED23 antibody	>1 publications with MED30 and MED23
MED21 antibody	>1 publications with MED30 and MED21
MED27 antibody	>1 publications with MED30 and MED27
MED11 antibody	>1 publications with MED30 and MED11
MED6 antibody	>1 publications with MED30 and MED6

Pathways associated with anti-MED30 antibody

Products by Pathway	Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies	Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Generic Transcription Pathway antibodies	Generic Transcription Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Lipids And Lipoproteins Pathway antibodies	Metabolism Of Lipids And Lipoproteins Pathway Diagram
PPARA Activates Gene Expression Pathway antibodies	PPARA Activates Gene Expression Pathway Diagram
Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway antibodies	Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway Diagram
Thyroid Hormone Signaling Pathway antibodies	Thyroid Hormone Signaling Pathway Diagram
Transcriptional Regulation Of White Adipocyte Differentiation Pathway antibodies	Transcriptional Regulation Of White Adipocyte Differentiation Pathway Diagram

Diseases associated with anti-MED30 antibody

Disease Name	Pubmed Publications
Breast Neoplasms Antibodies	>1 publications with MED30 and Breast Neoplasms
Nervous System Diseases Antibodies	>1 publications with MED30 and Nervous System Diseases

Organs/Tissues associated with anti-MED30 antibody

Organ/Tissue Name	Pubmed Publications
Heart Antibodies	>2 publications with MED30 and Heart
Mammary Gland Antibodies	>1 publications with MED30 and Mammary Gland