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Minichromosome Maintenance Complex Component 5 (MCM5) ELISA Kit for Bovine

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9339540

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! This kit is intended to be used for determination the level of MCM5 (hereafter termed this analyte) in undiluted original Bovine serum, plasma and tissue homogenate… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
minichromosome maintenance complex component 5 (MCM5), ELISA Kit
Full Product Name
Bovine DNA repliCation licensing factor MCM5, MCM5 ELISA Kit
Product Gene Name
Product Synonym Names
Bovine DNA replication licensing factor MCM5 (MCM5) ELISA kit; CDC46; MGC5315; P1-CDC46; DNA replication licensing factor; MCM5 minichromosome maintenance deficient 5; cell division cycle 46; minichromosome maintenance deficient 5 (cell division cycle 46) ; minichromosome maintenance complex component 5
Species Reactivity
Bovine
Assay Type
Sandwich
Samples
Serum, Plasma and Tissue Homogenate
Detection Range
0.25ng/ml-8ng/ml.
Sensitivity
0.1ng/ml.
Specificity
No significant cross-reactivity or interference between this analyte and analogues is observed.
Preparation and Storage
Store all reagents at 2-8 degree C
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Molecular Weight
82,146 Da
3D Structure
ModBase 3D Structure for Q0V8B7

Other Notes

Small volumes of MCM5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MCM5 purchase

MBS9339540 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the minichromosome maintenance complex component 5 (MCM5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MCM5. The ELISA analytical biochemical technique of the MBS9339540 kit is based on MCM5 antibody-MCM5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MCM5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MCM5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MCM5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA replication licensing factor MCM5
NCBI Official Synonym Full Names
minichromosome maintenance complex component 5<
NCBI Official Symbol
MCM5
UniProt Protein Name
DNA replication licensing factor MCM5
UniProt Gene Name
MCM5
UniProt Entry Name
MCM5_BOVIN
NCBI Protein Information
DNA replication licensing factor MCM5; minichromosome maintenance deficient protein 5

UniProt Comments for MCM5

Function: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity

Catalytic activity: ATP + H2O = ADP + phosphate.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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