Minichromosome Maintenance Deficient 2 (MCM2) ELISA Kit for Human

Name: Human MCM2 ELISA Kit; Minichromosome Maintenance Deficient 2; MyBioSource
Brand: MyBioSource
SKU: MBS089795
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS089795

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used to determine the level of MCM2 (hereafter termed "analyte") in… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Minichromosome Maintenance Deficient 2 (MCM2), ELISA Kit

Full Product Name

Human Minichromosome Maintenance Deficient 2 ELISA Kit

Product Gene Name

MCM2 elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Detection Range

0.25ng/ml-8ng/ml

Sensitivity

0.1ng/ml

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

101,896 Da

OMIM

116945

3D Structure

ModBase 3D Structure for P49736

Other Notes

Small volumes of MCM2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MCM2 purchase

MBS089795 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Minichromosome Maintenance Deficient 2 (MCM2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MCM2. The ELISA analytical biochemical technique of the MBS089795 kit is based on MCM2 antibody-MCM2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MCM2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MCM2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MCM2. It may not necessarily be applicable to this product.

NCBI GI #

119599745

NCBI GeneID

4171

NCBI Accession #

EAW79339.1

UniProt Primary Accession #

P49736

UniProt Secondary Accession #

Q14577; Q15023; Q8N2V1; Q969W7; Q96AE1; Q9BRM7

UniProt Related Accession #

P49736

NCBI Official Full Name

MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)

NCBI Official Synonym Full Names

minichromosome maintenance complex component 2

NCBI Official Symbol

MCM2

NCBI Official Synonym Symbols

BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN

UniProt Protein Name

DNA replication licensing factor MCM2

UniProt Synonym Protein Names

Minichromosome maintenance protein 2 homolog; Nuclear protein BM28

Protein Family

DNA replication licensing factor

UniProt Gene Name

MCM2

UniProt Synonym Gene Names

BM28; CCNL1; CDCL1; KIAA0030

UniProt Entry Name

MCM2_HUMAN

NCBI Protein Information

DNA replication licensing factor MCM2; cyclin-like 1; nuclear protein BM28; cell devision cycle-like 1; minichromosome maintenance protein 2 homolog; minichromosome maintenance deficient 2 (mitotin); MCM2 minichromosome maintenance deficient 2, mitotin

NCBI Summary for MCM2

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]

UniProt Comments for MCM2

MCM2: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division.

Protein type: DNA-binding; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: microtubule cytoskeleton; nucleoplasm; MCM complex; cytoplasm; nuclear origin of replication recognition complex; chromatin; nucleus

Molecular Function: protein binding; DNA helicase activity; DNA binding; histone binding; metal ion binding; DNA replication origin binding; ATP binding

Biological Process: DNA unwinding during replication; nucleosome assembly; DNA replication initiation; DNA strand elongation during DNA replication; mitotic cell cycle; DNA replication; cell cycle; G1/S transition of mitotic cell cycle

Research Articles on MCM2

1. ORC/Cdc6/MCM2-7 complex is a new regulatory mechanism for the helicase.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MCM2 elisa kit

Reference Product	PubMed Publications
MCM7 elisa kit	>106 publications with MCM2 and MCM7
MCM4 elisa kit	>101 publications with MCM2 and MCM4
MCM3 elisa kit	>90 publications with MCM2 and MCM3
MCM5 elisa kit	>85 publications with MCM2 and MCM5
CDC45 elisa kit	>84 publications with MCM2 and CDC45
MCM6 elisa kit	>79 publications with MCM2 and MCM6
CDC7 elisa kit	>70 publications with MCM2 and CDC7
DBF4 elisa kit	>43 publications with MCM2 and DBF4

Pathways associated with MCM2 elisa kit

Products by Pathway	Pathway Diagram
Activation Of ATR In Response To Replication Stress Pathway antibodies	Activation Of ATR In Response To Replication Stress Pathway Diagram
Activation Of The Pre-replicative Complex Pathway antibodies	Activation Of The Pre-replicative Complex Pathway Diagram
Assembly Of The Pre-replicative Complex Pathway antibodies	Assembly Of The Pre-replicative Complex Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Checkpoints Pathway antibodies	Cell Cycle Checkpoints Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
DNA Replication Pathway antibodies	DNA Replication Pathway Diagram

Diseases associated with MCM2 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>231 publications with MCM2 and Neoplasms
Adenocarcinoma Antibodies	>53 publications with MCM2 and Adenocarcinoma
Nervous System Diseases Antibodies	>21 publications with MCM2 and Nervous System Diseases
Precancerous Conditions Antibodies	>20 publications with MCM2 and Precancerous Conditions
Cell Transformation, Neoplastic Antibodies	>18 publications with MCM2 and Cell Transformation, Neoplastic
Breast Neoplasms Antibodies	>17 publications with MCM2 and Breast Neoplasms
Brain Diseases Antibodies	>17 publications with MCM2 and Brain Diseases
Neoplasms, Experimental Antibodies	>14 publications with MCM2 and Neoplasms, Experimental
Liver Diseases Antibodies	>13 publications with MCM2 and Liver Diseases
Hyperplasia Antibodies	>11 publications with MCM2 and Hyperplasia