Inhibitor of DNA Binding 1, Dominant Negative Helix-Loop-Helix (ID1) ELISA Kit for Human

Name: Human ID1 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS911748
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS911748

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ID1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ID1 present is bound by the… More details

$295 / 24-Strip-Wells (LIMIT 1) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

inhibitor of DNA binding 1, dominant negative helix-loop-helix protein (ID1), ELISA Kit

Full Product Name

Human DNA-binding protein inhibitor ID-1, ID1 ELISA Kit

Product Gene Name

ID1 elisa kit

Product Synonym Names

Human DNA-binding protein inhibitor ID-1 (ID1) ELISA kit; ID; bHLHb24; DNA-binding protein inhibitor ID-1; dJ857M17.1.2 (inhibitor of DNA binding 1; dominant negative helix-loop-helix protein) ; inhibitor of DNA binding 1; inhibitor of differentiation 1; inhibitor of DNA binding 1; dominant negative helix-loop-helix protein

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates, cell lysates

Detection Range

31.25 pg/ml-2000 pg/ml

Sensitivity

< 7.81 pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of human ID1. No significant cross-reactivity or interference between human ID1 and analogues was observed.

Preparation and Storage

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Inter-assay Precision

Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.

Molecular Weight

16,133 Da

OMIM

600349

3D Structure

ModBase 3D Structure for P41134

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ID1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ID1 purchase

MBS911748 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the inhibitor of DNA binding 1, dominant negative helix-loop-helix protein (ID1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ID1. The ELISA analytical biochemical technique of the MBS911748 kit is based on ID1 antibody-ID1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ID1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ID1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ID1. It may not necessarily be applicable to this product.

NCBI GI #

31317299

NCBI GeneID

3397

NCBI Accession #

NP_002156.2

UniProt Primary Accession #

P41134

UniProt Secondary Accession #

O00651; O00652; Q16371; Q16377; Q5TE66; Q5TE67; Q969Z7; Q9H0Z5; Q9H109; A8K537; E1P5L4

UniProt Related Accession #

P41134

GenBank Nucleotide #

NM_002165.3

NCBI Official Full Name

DNA-binding protein inhibitor ID-1 isoform a

NCBI Official Synonym Full Names

inhibitor of DNA binding 1, dominant negative helix-loop-helix protein

NCBI Official Symbol

ID1

NCBI Official Synonym Symbols

ID; bHLHb24

UniProt Protein Name

DNA-binding protein inhibitor ID-1

UniProt Synonym Protein Names

Class B basic helix-loop-helix protein 24; bHLHb24; Inhibitor of DNA binding 1; Inhibitor of differentiation 1

Protein Family

DNA-binding protein inhibitor

UniProt Gene Name

ID1

UniProt Synonym Gene Names

BHLHB24; ID; bHLHb24

UniProt Entry Name

ID1_HUMAN

NCBI Protein Information

DNA-binding protein inhibitor ID-1; inhibitor of differentiation 1; class B basic helix-loop-helix protein 24; dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein)

NCBI Summary for ID1

The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for ID1

ID1: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 20q11

Cellular Component: nucleoplasm; Golgi apparatus; nucleus

Molecular Function: protein dimerization activity; protein binding; transcription factor activity

Biological Process: circadian rhythm; transcription, DNA-dependent; collagen metabolic process; protein destabilization; blood vessel endothelial cell migration; heart development; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; regulation of angiogenesis; negative regulation of DNA binding; response to antibiotic; BMP signaling pathway; transforming growth factor beta receptor signaling pathway; regulation of MAPKKK cascade; blood vessel morphogenesis; negative regulation of osteoblast differentiation; endothelial cell morphogenesis; angiogenesis; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis

Research Articles on ID1

1. Id1 induces mesenchymal-to-epithelial transition and pulmonary metastatic colonization by antagonizing Twist1 activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ID1 elisa kit

Reference Product	PubMed Publications
TCF3 elisa kit	>25 publications with ID1 and TCF3
HES1 elisa kit	>10 publications with ID1 and HES1
MYOG elisa kit	>9 publications with ID1 and MYOG
MYOD1 elisa kit	>8 publications with ID1 and MYOD1
TCF4 elisa kit	>8 publications with ID1 and TCF4
TCF12 elisa kit	>6 publications with ID1 and TCF12
MYF5 elisa kit	>4 publications with ID1 and MYF5
IFI16 elisa kit	>3 publications with ID1 and IFI16
CASK elisa kit	>3 publications with ID1 and CASK
PSMD4 elisa kit	>1 publications with ID1 and PSMD4

Pathways associated with ID1 elisa kit

Products by Pathway	Pathway Diagram
ALK1 Signaling Events Pathway antibodies	ALK1 Signaling Events Pathway Diagram
IL-3 Signaling Pathway antibodies	IL-3 Signaling Pathway Diagram
Id Signaling Pathway antibodies	Id Signaling Pathway Diagram
Notch-mediated HES/HEY Network Pathway antibodies	Notch-mediated HES/HEY Network Pathway Diagram
TGF-beta Signaling Pathway antibodies	TGF-beta Signaling Pathway Diagram
TGF-beta Signaling Pathway antibodies	TGF-beta Signaling Pathway Diagram

Diseases associated with ID1 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>279 publications with ID1 and Neoplasms
Carcinoma Antibodies	>91 publications with ID1 and Carcinoma
Skin Diseases Antibodies	>53 publications with ID1 and Skin Diseases
Breast Neoplasms Antibodies	>48 publications with ID1 and Breast Neoplasms
Neoplasm Metastasis Antibodies	>38 publications with ID1 and Neoplasm Metastasis
Prostatic Neoplasms Antibodies	>37 publications with ID1 and Prostatic Neoplasms
Adenocarcinoma Antibodies	>35 publications with ID1 and Adenocarcinoma
Cell Transformation, Neoplastic Antibodies	>34 publications with ID1 and Cell Transformation, Neoplastic
Neoplasms, Experimental Antibodies	>28 publications with ID1 and Neoplasms, Experimental
Lung Diseases Antibodies	>25 publications with ID1 and Lung Diseases

Organs/Tissues associated with ID1 elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>195 publications with ID1 and Bone
Blood Antibodies	>134 publications with ID1 and Blood
Vascular Antibodies	>91 publications with ID1 and Vascular
Muscle Antibodies	>87 publications with ID1 and Muscle
Brain Antibodies	>57 publications with ID1 and Brain
Embryonic Tissue Antibodies	>54 publications with ID1 and Embryonic Tissue
Prostate Antibodies	>51 publications with ID1 and Prostate
Liver Antibodies	>47 publications with ID1 and Liver
Lung Antibodies	>46 publications with ID1 and Lung
Nerve Antibodies	>31 publications with ID1 and Nerve