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EGFL7 (EGFL) Human Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553252

EGFL7 is an 30 kDa secreted protein that contain an Emilin-like (EMI) domain, and 2 epidermal growth factor (EGF) domains, one of which binds calcium. Based on these domains, it has been hypothesized that EGFL7 may self-assemble like extracellular matrix… More details

$335 / 0.005 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
EGFL7, Active Protein
Full Product Name
Human EGFL7 Recombinant
Product Gene Name
EGFL active protein
Product Synonym Gene Name
EGF
Product Synonym Names
ve-statin; EGFL7
Host
Insect Cells (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Recombinant Human EGFL7 is lyophilized 0.2 um filtered 100mM glycine, 10mM NaCl, 10% Glycerol solution pH 7.0 and 5% trehalose.
Purity/Purification
>90%, as determined by SDS-PAGE
Preparation and Storage
Lyophilized EGFL7, as supplied, should be stored at -20 degree C to -70 degree C.
Upon reconstitution, working aliquots may be stored at 2 - 8 degree C for one month, or six months at -20 degree C without detecable loss of activity. It is recommended to add 0.1% BSA for long term storage. Avoid freeze/thaw cycles.
Molecular Weight
29,618 Da
OMIM
608582
3D Structure
ModBase 3D Structure for Q9UHF1
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Domain
EGF,EMI
Host Note
DNA sequence encoding mature chain of Human EGFL7 (VE-Statin) to a C-terminal polyHis tag was expressed in Insect cells.
Biological Activity
The activity was determined by the ability to bind human NOTCH1 in functional ELISA.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1mg/ml. It is recommended to add 0.1% BSA for long term storage of reconstituted product.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Molecular Weight Note
Recombinant Human EGFL7 is a monomer consisting of 268 amino acid residue, and migrates as an approximately 31 kDa protein under reducing conditions in SDS-PAGE.

Product Categories/Family for EGFL active protein

Cytokines

Other Notes

Small volumes of EGFL active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EGFL purchase

MBS553252 is an active protein targeting EGFL. Also known as Human EGFL7 Recombinant. Host: Insect Cells (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders).

Additional Details

NCBI/Uniprot data below describe general gene information for EGFL. It may not necessarily be applicable to this product.

NCBI GI #
7705889
NCBI GeneID
51162
NCBI Accession #
NP_057299.1
UniProt Primary Accession #
Q9UHF1
UniProt Secondary Accession #
Q5M7Y5; Q5VUD5; Q96EG0; B3KRP0
GenBank Nucleotide #
NM_016215.4
NCBI Official Full Name
epidermal growth factor-like protein 7
UniProt Protein Name
Epidermal growth factor-like protein 7
UniProt Synonym Protein Names
Multiple epidermal growth factor-like domains protein 7; Multiple EGF-like domains protein 7; NOTCH4-like protein; Vascular endothelial statin; VE-statin; Zneu1
UniProt Gene Name
EGFL7
UniProt Synonym Gene Names
MEGF7; EGF-like protein 7; Multiple EGF-like domains protein 7; VE-statin
UniProt Entry Name
EGFL7_HUMAN

NCBI Summary for EGFL

This gene encodes a secreted endothelial cell protein that contains two epidermal growth factor-like domains. The encoded protein may play a role in regulating vasculogenesis. This protein may be involved in the growth and proliferation of tumor cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]
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UniProt Comments for EGFL

EGFL7: Regulates vascular tubulogenesis in vivo. Inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cells adhesion to the substrate in vitro.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: calcium ion binding; Notch binding

Biological Process: blood vessel development; negative regulation of Notch signaling pathway; positive regulation of endothelial cell proliferation; angiogenesis; vasculogenesis; cell adhesion; negative regulation of cell migration
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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