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Osteopontin (OPN) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2153399

MBS2153399 is an active protein targeting OPN. Also known as Active Osteopontin (OPN). Host: 293F Cell. More details

$325 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Osteopontin (OPN), Active Protein
Full Product Name
Active Osteopontin (OPN)
Product Gene Name
OPN active protein
Product Synonym Names
Osteopontin; OPN active protein (eukaryotic, 293F Cell), Mouse; SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin
Host
293F Cell
Form/Format
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Freeze-dried powder
Purity/Purification
Greater than 95% by SDS-PAGE
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Stable for 12 months if correctly stored.
Molecular Weight
32,459 Da
Sequence Positions
Leu17~Asn294
3D Structure
ModBase 3D Structure for P10923
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Expression System
Eukaryotic expression
Species
Mouse
Tag
N-terminal His
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
4.3
Research Area
Tumor immunity

Product Categories/Family for OPN active protein

Active proteins
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for OPN active protein
Cell culture, Activity Assay

SDS-PAGE of OPN active protein

Figure. SDS-PAGE
OPN Active Protein – Cat# MBS2153399

Activity of OPN active protein

Osteopontin (OPN), a multifunctional phosphorylated glycoprotein, plays an important role in neutrophil recruitment and was found to induce the expression of proinflammatory chemokines including MCP-1 and MIP-1beta which promote migration and recruitment
OPN Active Protein, Activity – Cat# MBS2153399

Other Notes

Small volumes of OPN active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for OPN. It may not necessarily be applicable to this product.

NCBI GI #
129261
NCBI GeneID
20750
NCBI Accession #
P10923.1
UniProt Primary Accession #
P10923
UniProt Secondary Accession #
P19008; Q91VH4
UniProt Related Accession #
P10923
NCBI Official Full Name
Osteopontin
NCBI Official Synonym Full Names
secreted phosphoprotein 1
NCBI Official Symbol
Spp1
NCBI Official Synonym Symbols
OP; 2AR; Bsp; Eta; Opn; Ric; BNSP; BSPI; Opnl; Apl-1; ETA-1; Spp-1
UniProt Protein Name
Osteopontin
UniProt Synonym Protein Names
2AR; Bone sialoprotein 1; Calcium oxalate crystal growth inhibitor protein; Early T-lymphocyte activation 1 protein; Minopontin; Secreted phosphoprotein 1; SPP-1
UniProt Gene Name
Spp1
UniProt Synonym Gene Names
Eta-1; Op; Spp-1; SPP-1
UniProt Entry Name
OSTP_MOUSE
NCBI Protein Information
osteopontin; minopontin; bone sialoprotein 1; osteopontin-like protein; 44 kDa bone phosphoprotein; early T-lymphocyte activation 1 protein; calcium oxalate crystal growth inhibitor protein

UniProt Comments for OPN

osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; cell projection; perinuclear region of cytoplasm; apical part of cell; cytoplasm; extracellular region

Molecular Function: extracellular matrix binding; cytokine activity

Biological Process: response to organic substance; osteoblast differentiation; neutrophil chemotaxis; ossification; response to vitamin D; response to steroid hormone stimulus; biomineral formation; positive regulation of bone resorption; cell adhesion; negative regulation of apoptosis
Show more

Research Articles on OPN

1. Hepatic ischemia reperfusion injury is aggravated in osteopontin knockout mice.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with OPN active protein

 Products by Pathway  Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Integrin Cell Surface Interactions Pathway antibodies  Integrin Cell Surface Interactions Pathway Diagram
 Osteoclast Pathway antibodies  Osteoclast Pathway Diagram

Diseases associated with OPN active protein

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >259 publications with OPN and Cardiovascular Diseases
 Inflammation Antibodies  >233 publications with OPN and Inflammation
 Kidney Diseases Antibodies  >225 publications with OPN and Kidney Diseases
 Fibrosis Antibodies  >148 publications with OPN and Fibrosis
 Neoplasm Metastasis Antibodies  >133 publications with OPN and Neoplasm Metastasis
 Necrosis Antibodies  >123 publications with OPN and Necrosis
 Calcinosis Antibodies  >109 publications with OPN and Calcinosis
 Adenocarcinoma Antibodies  >102 publications with OPN and Adenocarcinoma
 Heart Diseases Antibodies  >100 publications with OPN and Heart Diseases
 Liver Diseases Antibodies  >84 publications with OPN and Liver Diseases

Organs/Tissues associated with OPN active protein

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >1056 publications with OPN and Bone
 Blood Antibodies  >522 publications with OPN and Blood
 Kidney Antibodies  >361 publications with OPN and Kidney
 Connective Tissue Antibodies  >326 publications with OPN and Connective Tissue
 Muscle Antibodies  >276 publications with OPN and Muscle
 Bone Marrow Antibodies  >186 publications with OPN and Bone Marrow
 Heart Antibodies  >128 publications with OPN and Heart
 Liver Antibodies  >122 publications with OPN and Liver
 Lung Antibodies  >101 publications with OPN and Lung
 Embryonic Tissue Antibodies  >98 publications with OPN and Embryonic Tissue
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