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PRDX5 Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS201064

Peroxiredoxin 5, also known as PRDX5, is a member of the peroxiredoxin family of antioxidant enzymes, which reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. This protein may play an… More details

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Product Overview

Online Datasheet in PDF
Product Name
PRDX5, Active Protein
Full Product Name
Recombinant PRDX5
Product Gene Name
Product Synonym Names
Peroxiredoxin 5; mitochondrial isoform L; ACR1; AOEB166; B166; HEL-S-55; PLP; PMP20; PRDX6; prx-V; PRXV; SBBI10
Sequence
MAPIKVGDAI PAVEVFEGEP GNKVNLAELF KGKKGVLFGV PGAFTPGCSK THLPGFVEQA EALKAKGVQV VACLSVNDAF VTGEWGRAHK AEGKVRLLAD PTGAFGKETD LLLDDSLVSI FGNRRLKRFS MVVQDGIVKA LNVEPDGTGL TCSLAPNIIS QL
Host
E Coli
Form/Format
Liquid; In 20mM HEPES (pH7.4).
Purity/Purification
>95% by SDS-PAGE
Concentration
1mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
Molecular Weight
17kDa (162aa) confirmed by MALDI-TOF
Sequence Positions
53-214aa
OMIM
606583
3D Structure
ModBase 3D Structure for P30044
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Species
Human
Endotoxin
<1.0 EU per 1ug of protein (determined by LAL method)
Tag
Non-Tagged
Biological Activity
Specific activity is >9,000 pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for 1minute.
Applications Tested/Suitable for PRDX5 active protein
Enzyme Activity, SDS-PAGE

SDS-Page of PRDX5 active protein

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
PRDX5 Active Protein – Cat# MBS201064

Product References and Citations for PRDX5 active protein

Yamashita H., et al. (1999) J Biol Chem. 274(42):29897-904Declercg JP., et al. (2001) J Mol Biol. 311(4):751-9

Other Notes

Small volumes of PRDX5 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRDX5 purchase

MBS201064 is an active protein targeting PRDX5. Also known as Recombinant PRDX5. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for PRDX5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
peroxiredoxin-5, mitochondrial isoform L
NCBI Official Synonym Full Names
peroxiredoxin 5
NCBI Official Symbol
PRDX5
NCBI Official Synonym Symbols
PLP; ACR1; B166; PRXV; PMP20; PRDX6; prx-V; SBBI10; AOEB166; HEL-S-55
UniProt Protein Name
Peroxiredoxin-5, mitochondrial
UniProt Synonym Protein Names
Alu corepressor 1; Antioxidant enzyme B166; AOEB166; Liver tissue 2D-page spot 71B; PLP; Peroxiredoxin V; Prx-V; Peroxisomal antioxidant enzyme; TPx type VI; Thioredoxin peroxidase PMP20; Thioredoxin reductase
Protein Family
UniProt Gene Name
PRDX5
UniProt Synonym Gene Names
ACR1; AOEB166; Prx-V
UniProt Entry Name
PRDX5_HUMAN
NCBI Protein Information
peroxiredoxin-5, mitochondrial

NCBI Summary for PRDX5

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein interacts with peroxisome receptor 1 and plays an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. The use of alternate transcription start sites is thought to result in transcript variants that use different in-frame translational start codons to generate isoforms that are targeted to the mitochondrion (isoform L) or peroxisome/cytoplasm (isoform S). Multiple related pseudogenes have been defined for this gene. [provided by RefSeq, Nov 2017]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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