Thrombin Active Protein (Bovine Plasma)

This active thrombin protein, derived from bovine plasma, functions as a serine protease (EC 3.4.21.5) that selectively cleaves Arg–Gly bonds in fibrinogen to generate fibrin, and is widely used in coagulation cascade studies, recombinant protein tag removal, and fibrin clot formation assays.

MyBioSource producer and supplier of bovine Thrombin, medical research of proteolytic enzymes and manufacturing of defibrinating human plasma and serum free media component.

Custom preparations, technical support, bulk quantities and aliquoting available.

Bovine Thrombin EC 3.4.21.5 (activated Factor II) is a coagulation protein that has many effects in the coagulation cascade. Bovine Thrombin is a serine protease that converts fibrinogen into fibrin.

Bovine Thrombin used as a reagent, Thrombin has proven useful in the laboratory evaluation of many fibrinogen(s)

Our customers use Bovine Thrombin to defibrinate human plasma, producing serum matrixes to be used as controls and standards. Bovine Thrombin High specific material available (> 2000 U/mg protein) is used to cleave r-fusion proteins containing a thrombin site for removal of affinity tags as well as certain pharmaceutical applications.

Thrombin promotes chemotaxis in monocytes, neurite retraction in neuron Thrombin serves as a potent mitogenic agent in fibroblast growth and functions as a serum-free media component for several cell lines

Bovine Thrombin is a multifunctional proteolytic enzyme which acts both specifically and non-specifically with several proteins, cells, surfaces, and other molecules. Bovine Thrombin(s) essential role in the clotting process has been well characterized where it converts fibrinogen, a soluble plasma glycoprotein, to an insoluble protein gel called fibrin, the essential component of the blood clot.

Small volumes of thrombin active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

By acting as a serine protease enzyme, thrombin converts the soluble protein fibrinogen into a matrix forming insoluble complexed clotting and coagulation factor, fibrin. Thrombin is derived from protease cleavage of prothrombin, through vitamin K and calcium binding dependent factors. Inhibition of these amino acids removal in blood by thrombin inhibitors of the thrombin active site results in loss of ability to clot or to activate platelet receptor proteins required for aggregation. Cell research into active site thrombin complex structure, discovery of potential new thrombin inhibitor complex or understanding its crystal structure and crystallographic structure thrombin complex, further require a purified source of thrombin. A purified bovine thrombin, with activities ranging from 50,000 - 150,000 US U/gram. is available as an economical Standard grade lyophilized protein for multiple research laboratory protocols, such as defibrinate human alpha fibrinogen from plasma or removal of affinity tags.