Human PFKL cDNA Clone Expression Plasmid – Cat# MBS1267384

PFKL cDNA Clone – Phosphofructokinase, Liver (Human)

Name: PFKL cDNA Clone; Phosphofructokinase, Liver; Human; MyBioSource
Brand: MyBioSource
SKU: MBS1267384
Price: 520 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1267384

MBS1267384 is a cDNA clone targeting PFKL. More details

$520 / 0.01 mg Plasmid + 0.2 mL Glycerol-Stock +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PFKL, cDNA Clone

Full Product Name

PFKL cDNA Clone

Product Gene Name

PFKL cdna clone

Sequence

atgtgtaacc agggtagagg tcgagagtcc tctcgtgggg gtctccatgt tcaagggagc tgccgaggct tgagcaggag cccccagcag gaaactggct ttgccaaggc ccccgctggg acagactgtt tctttcactg cagtcctggg agccgagggc aaggggacag gaaagaggaa gtgacctcag agcctggtgg caccagcatc atgtccaggc tggggggcat gaacgctgct gtccgggctg tgacgcgcat gggcatttat gtgggtgcca aagtcttcct catctacgag ggctatgagg gcctcgtgga gggaggtgag aacatcaagc aggccaactg gctgagcgtc tccaacatca tccagctggg cggcactatc attggcagcg ctcgctgcaa ggcctttacc accagggagg ggcgccgggc agcggcctac aacctggtcc agcacggcat caccaacctg tgcgtcatcg gcggggatgg cagccttaca ggtgccaaca tcttccgcag cgagtggggc agcctgctgg aggagctggt ggcggaaggt aagatctcag agactacagc ccggacctac tcgcacctga acatcgcggg cctagtgggc tccatcgata acgacttctg cggcaccgac atgaccatcg gcacggactc ggccctccac cgcatcatgg aggtcatcga tgccatcacc accactgccc agagccacca gaggaccttc gtgctggaag tgatgggccg gcactgcggg tacctggcgc tggtatctgc actggcctca ggggccgact ggctgttcat ccccgaggct ccacccgagg acggctggga gaacttcatg tgtgagaggc tgggtgagac tcggagccgt gggtcccgac tgaacatcat catcatcgct gagggtgcca ttgaccgcaa cgggaagccc atctcgtcca gctacgtgaa ggacctggtg gttcagaggc tgggcttcga cacccgtgta actgtgctgg gccacgtgca gcggggaggg acgccctctg ccttcgaccg gatcctgagc agcaagatgg gcatggaggc ggtgatggcg ctgctggaag ccacgcctga cacgccggcc tgcgtggtca ccctctcggg gaaccagtca gtgcggctgc ccctcatgga gtgcgtgcag atgaccaagg aagtgcagaa agccatggat gacaagaggt ttgacgaggc cacccagctc cgtggtggga gcttcgagaa caactggaac atttacaagc tcctcgccca ccagaagccc cccaaggaga agtctaactt ctccctggcc atcctgaatg tgggggcccc ggcggctggc atgaatgcgg ccgtgcgctc ggcggtgcgg accggcatct cccatggaca cacagtatac gtggtgcacg atggcttcga aggcctagcc aagggtcagg tgcaagaagt aggctggcac gacgtggccg gctggttggg gcgtggtggc tccatgctgg ggaccaagag gaccctgccc aagggccagc tggagtccat tgtggagaac atccgcatct atggtattca cgccctgctg gtggtcggtg ggtttgaggc ctatgaaggg gtgctgcagc tggtggaggc tcgcgggcgc tacgaggagc tctgcatcgt catgtgtgtc atcccagcca ccatcagcaa caacgtccct ggcaccgact tcagcctggg ctccgacact gctgtaaatg ccgccatgga gagctgtgac cgcatcaaac agtctgcctc ggggaccaag cgccgtgtgt tcatcgtgga gaccatgggg ggttactgtg gctacctggc caccgtgact ggcattgctg tgggggccga cgccgcctac gtcttcgagg accctttcaa catccacgac ttaaaggtca acgtggagca catgacggag aagatgaaga cagacattca gaggggcctg gtgctgcgga acgagaagtg ccatgactac tacaccacgg agttcctgta caacctgtac tcatcagagg gcaagggcgt cttcgactgc aggaccaatg tcctgggcca cctgcagcag ggtggcgctc caaccccctt tgaccggaac tatgggacca agctgggggt gaaggccatg ctgtggttgt cggagaagct gcgcgaggtt taccgcaagg gacgggtgtt cgccaatgcc ccagactcgg cctgcgtgat cggcctgaag aagaaggcgg tggccttcag ccccgtcact gagctcaaga aagacactga tttcgagcac cgcatgccac gggagcagtg gtggctgagc ctgcggctca tgctgaagat gctggcacaa taccgcatca gtatggccgc ctacgtgtca ggggagctgg agcacgtgac ccgccgcacc ctgagcatgg acaagggctt ctga

Molecular Weight

90,203 Da

OMIM

171860

Vector

pENTR223.1 or pUC

Clone Sequence Report

Provided with product shipment

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PFKL cdna clone vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PFKL. It may not necessarily be applicable to this product.

NCBI GI #

13623608

NCBI GeneID

5211

NCBI Accession #

BC006422

UniProt Secondary Accession #

Q96A64; Q96IH4; Q9BR91

UniProt Related Accession #

P17858

NCBI Official Full Name

Homo sapiens phosphofructokinase, liver, mRNA

NCBI Official Synonym Full Names

phosphofructokinase, liver type

NCBI Official Symbol

PFKL

NCBI Official Synonym Symbols

PFK-B; PFK-L; ATP-PFK

UniProt Protein Name

ATP-dependent 6-phosphofructokinase, liver type

UniProt Synonym Protein Names

6-phosphofructokinase type B; Phosphofructo-1-kinase isozyme B; PFK-B; Phosphohexokinase

Protein Family

ATP-dependent 6-phosphofructokinase

UniProt Gene Name

PFKL

UniProt Synonym Gene Names

ATP-PFK; PFK-L

UniProt Entry Name

PFKAL_HUMAN

NCBI Protein Information

ATP-dependent 6-phosphofructokinase, liver type

NCBI Summary for PFKL

This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

UniProt Comments for PFKL

PFKL: phosphofructokinase, liver type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts. Two alternatively spliced isoforms have been described.

Protein type: Carbohydrate Metabolism - galactose; Kinase, other; EC 2.7.1.11; Carbohydrate Metabolism - pentose phosphate pathway; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - fructose and mannose

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: 6-phosphofructokinase complex; cytosol; membrane

Molecular Function: 6-phosphofructokinase activity; ATP binding; identical protein binding; kinase binding; protein binding

Biological Process: fructose 1,6-bisphosphate metabolic process; fructose 6-phosphate metabolic process; glycolysis; protein oligomerization; response to glucose stimulus

Research Articles on PFKL

1. findings showed glycosylation inhibited PFK1 activity and redirected glucose flux through the pentose phosphate pathway, thereby conferring a selective growth advantage on cancer cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with PFKL cdna clone

Products by Pathway	Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram
Fructose And Mannose Metabolism Pathway antibodies	Fructose And Mannose Metabolism Pathway Diagram
Fructose And Mannose Metabolism Pathway antibodies	Fructose And Mannose Metabolism Pathway Diagram
Galactose Metabolism Pathway antibodies	Galactose Metabolism Pathway Diagram
Galactose Metabolism Pathway antibodies	Galactose Metabolism Pathway Diagram
Glucose Metabolism Pathway antibodies	Glucose Metabolism Pathway Diagram
Glycolysis Pathway antibodies	Glycolysis Pathway Diagram
Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies	Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram
Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies	Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram

Diseases associated with PFKL cdna clone

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>22 publications with PFKL and Nervous System Diseases
Congenital Abnormalities Antibodies	>13 publications with PFKL and Congenital Abnormalities
Brain Diseases Antibodies	>9 publications with PFKL and Brain Diseases
Immune System Diseases Antibodies	>5 publications with PFKL and Immune System Diseases
Cardiovascular Diseases Antibodies	>3 publications with PFKL and Cardiovascular Diseases
Heart Diseases Antibodies	>2 publications with PFKL and Heart Diseases
Insulin Resistance Antibodies	>1 publications with PFKL and Insulin Resistance
Death Antibodies	>1 publications with PFKL and Death
Hypertension Antibodies	>1 publications with PFKL and Hypertension
Liver Diseases Antibodies	>1 publications with PFKL and Liver Diseases

Organs/Tissues associated with PFKL cdna clone

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>25 publications with PFKL and Liver
Muscle Antibodies	>9 publications with PFKL and Muscle
Brain Antibodies	>6 publications with PFKL and Brain
Blood Antibodies	>4 publications with PFKL and Blood
Embryonic Tissue Antibodies	>2 publications with PFKL and Embryonic Tissue
Heart Antibodies	>2 publications with PFKL and Heart
Pancreas Antibodies	>1 publications with PFKL and Pancreas
Kidney Antibodies	>1 publications with PFKL and Kidney
Vascular Antibodies	>1 publications with PFKL and Vascular
Thymus Antibodies	>1 publications with PFKL and Thymus