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CD38 Cell Lysate – Tissue Lysate

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8114159

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more… More details

$315 / 0.3 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
CD38, Cell Lysate
Full Product Name
Human CD38 HEK293 Overexpression Lysate
Product Gene Name
CD38 cell lysate
Product Synonym Names
Human CD38 HEK293 Cell Lysate (WB positive control); CD38 molecule; Human ADPRC 1 Overexpression Lysate; Human ADPRC1 Overexpression Lysate; Human T10 Overexpression Lysate
Sequence
Val43-Ile3 00
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Molecular Weight
13,788 Da
Sequence Positions
269aa
OMIM
107270
3D Structure
ModBase 3D Structure for P28907
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the extracellular domain of human CD38 (NP_001766.2) (Val 43-Ile 300) with a C-terminal polyhistidine tag was expressed.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Product Categories/Family for CD38 cell lysate

Cancer Drug Targets
Applications Tested/Suitable for CD38 cell lysate
Western Blot (WB)
Application Notes for CD38 cell lysate
WB: Use at an assay dependent dilution.

Product References and Citations for CD38 cell lysate

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.

Other Notes

Small volumes of CD38 cell lysate vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD38 purchase

MBS8114159 is a cell lysate targeting CD38. Also known as Human CD38 HEK293 Overexpression Lysate. Host: HEK293 Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for CD38. It may not necessarily be applicable to this product.

NCBI GI #
38454326
NCBI GeneID
952
NCBI Accession #
NP_001766.2
UniProt Primary Accession #
P28907
UniProt Secondary Accession #
O00121; O00122; Q96HY4
UniProt Related Accession #
P28907
GenBank Nucleotide #
NM_001775.3
NCBI Official Full Name
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
NCBI Official Synonym Full Names
CD38 molecule
NCBI Official Symbol
CD38
NCBI Official Synonym Symbols
ADPRC1; ADPRC 1
UniProt Protein Name
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
UniProt Synonym Protein Names
2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase (EC:2.4.99.20); 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADPRC 1; Cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; T10; CD_antigen: CD38
Protein Family
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase
UniProt Gene Name
CD38
UniProt Synonym Gene Names
ADPRC 1; cADPr hydrolase 1
UniProt Entry Name
CD38_HUMAN
NCBI Protein Information
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1

NCBI Summary for CD38

The protein encoded by this gene is a non-lineage-restricted, type II transmembrane glycoprotein that synthesizes and hydrolyzes cyclic adenosine 5'-diphosphate-ribose, an intracellular calcium ion mobilizing messenger. The release of soluble protein and the ability of membrane-bound protein to become internalized indicate both extracellular and intracellular functions for the protein. This protein has an N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites. Crystal structure analysis demonstrates that the functional molecule is a dimer, with the central portion containing the catalytic site. It is used as a prognostic marker for patients with chronic lymphocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
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UniProt Comments for CD38

CD38: Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. Belongs to the ADP-ribosyl cyclase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.2.2.6; EC 2.4.99.20; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Hydrolase; Cell surface; Apoptosis; Lyase; Membrane protein, integral; Cell cycle regulation

Chromosomal Location of Human Ortholog: 4p15

Cellular Component: cell surface; membrane; integral to membrane; plasma membrane; nucleus

Molecular Function: transferase activity; phosphorus-oxygen lyase activity; NAD+ nucleosidase activity; NAD(P)+ nucleosidase activity

Biological Process: response to drug; response to retinoic acid; metabolic process; positive regulation of transcription, DNA-dependent; positive regulation of insulin secretion; female pregnancy; signal transduction; positive regulation of cell growth; response to estradiol stimulus; B cell receptor signaling pathway; elevation of cytosolic calcium ion concentration; response to hydroperoxide; positive regulation of B cell proliferation; positive regulation of vasoconstriction; response to hypoxia; response to progesterone stimulus; negative regulation of transcription, DNA-dependent; negative regulation of bone resorption; negative regulation of apoptosis
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Research Articles on CD38

1. Results may imply that CD38 expression either reflects or participates in pathogenic mechanisms of HIV disease independently of cell cycling.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CD38 cell lysate

 Reference Product  PubMed Publications
 CD4 cell lysate  >1344 publications with CD38 and CD4
 ZAP70 cell lysate  >256 publications with CD38 and ZAP70
 LCK cell lysate  >8 publications with CD38 and LCK
 BST1 cell lysate  >3 publications with CD38 and BST1
 CD8A cell lysate  >2 publications with CD38 and CD8A
 NNMT cell lysate  >1 publications with CD38 and NNMT
 ENPP1 cell lysate  >1 publications with CD38 and ENPP1

Pathways associated with CD38 cell lysate

 Products by Pathway  Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
 Oxytocin Signaling Pathway antibodies  Oxytocin Signaling Pathway Diagram

Diseases associated with CD38 cell lysate

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >487 publications with CD38 and Cardiovascular Diseases
 Inflammation Antibodies  >317 publications with CD38 and Inflammation
 Necrosis Antibodies  >214 publications with CD38 and Necrosis
 Nervous System Diseases Antibodies  >115 publications with CD38 and Nervous System Diseases
 Liver Diseases Antibodies  >111 publications with CD38 and Liver Diseases
 Disease Models, Animal Antibodies  >82 publications with CD38 and Disease Models, Animal
 Lung Diseases Antibodies  >78 publications with CD38 and Lung Diseases
 Carcinoma Antibodies  >71 publications with CD38 and Carcinoma
 Breast Neoplasms Antibodies  >44 publications with CD38 and Breast Neoplasms
 Fever Antibodies  >37 publications with CD38 and Fever

Organs/Tissues associated with CD38 cell lysate

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3989 publications with CD38 and Blood
 Bone Marrow Antibodies  >1457 publications with CD38 and Bone Marrow
 Lymph Node Antibodies  >297 publications with CD38 and Lymph Node
 Liver Antibodies  >234 publications with CD38 and Liver
 Lymph Antibodies  >223 publications with CD38 and Lymph
 Spleen Antibodies  >175 publications with CD38 and Spleen
 Muscle Antibodies  >144 publications with CD38 and Muscle
 Brain Antibodies  >135 publications with CD38 and Brain
 Tonsil Antibodies  >117 publications with CD38 and Tonsil
 Pharynx Antibodies  >110 publications with CD38 and Pharynx
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