KLRC2 Cell Lysate – Tissue Lysate

Name: KLRC2 Cell Lysate; Tissue Lysate; MyBioSource
Brand: MyBioSource
SKU: MBS8119783
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8119783

Description: This Human KLRC2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of KLRC2 protein from the overexpression lysate was verified. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

KLRC2, Cell Lysate

Full Product Name

Human KLRC2 HEK293 Overexpression Lysate

Product Gene Name

KLRC2 cell lysate

Product Synonym Names

Human KLRC2 HEK293 Cell Lysate (WB positive control); Human CD159c Overexpression Lysate; Human NKG2-C Overexpression Lysate; Human NKG2C Overexpression Lysate

Host

HEK293 Cells

Form/Format

Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).

Preparation and Storage

Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.

Molecular Weight

The recombinant human KLRC2 consists of 375 amino acids and predicts a molecular mass of 42.4kDa. As a result of glycosylation, it migrates as an approximately 53.2kDa in SDS-PAGE under reducing conditions.

Species

Human

Sequence Information

A DNA sequence encoding the human KLRC2 (P26717-1)(Ile94-Leu231) was expressed with the Fc region of human IgG1 at the N-terminus.

Preparation Method

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Recommend Usage

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
3. Store the lyophilized cell lysate at 4 degree C. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80 degree C.

Applications Tested/Suitable for KLRC2 cell lysate

Western Blot (WB)

Other Notes

Small volumes of KLRC2 cell lysate vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KLRC2 purchase

MBS8119783 is a cell lysate targeting KLRC2. Also known as Human KLRC2 HEK293 Overexpression Lysate. Host: HEK293 Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for KLRC2. It may not necessarily be applicable to this product.

Protein Family

NKG2-C type II integral membrane protein

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.