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CETN2 Cell Lysate – Tissue Lysate

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8115439

This Human Centrin 2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Centrin 2 protein from the overexpression lysate was verified. More details

$315 / 0.3 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Centrin 2 (CETN2), Cell Lysate
Full Product Name
Human Centrin 2 HEK293 Overexpression Lysate
Product Gene Name
CETN2 cell lysate
Product Synonym Names
Human CETN2/Centrin 2 HEK293 Cell Lysate (WB positive control); centrin, EF-hand protein, 2; Human CALT Overexpression Lysate; Human CEN2 Overexpression Lysate
Sequence
Met1-Tyr17 2
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Molecular Weight
19,738 Da
Sequence Positions
183aa
OMIM
300006
3D Structure
ModBase 3D Structure for P41208
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human CETN2 (NP_004335.1) (Met1-Tyr172) was expressed with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Applications Tested/Suitable for CETN2 cell lysate
Western Blot (WB)
Application Notes for CETN2 cell lysate
WB: Use at an assay dependent dilution.

Other Notes

Small volumes of CETN2 cell lysate vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CETN2 purchase

MBS8115439 is a cell lysate targeting CETN2. Also known as Human Centrin 2 HEK293 Overexpression Lysate. Host: HEK293 Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for CETN2. It may not necessarily be applicable to this product.

NCBI GI #
4757902
NCBI GeneID
1069
NCBI Accession #
NP_004335.1
UniProt Primary Accession #
P41208
UniProt Secondary Accession #
Q53XW1; B2R4T4
UniProt Related Accession #
P41208
GenBank Nucleotide #
NM_004344.1
NCBI Official Full Name
centrin-2
NCBI Official Synonym Full Names
centrin, EF-hand protein, 2
NCBI Official Symbol
CETN2
NCBI Official Synonym Symbols
CALT; CEN2
UniProt Protein Name
Centrin-2
UniProt Synonym Protein Names
Caltractin isoform 1
Protein Family
Centrin
UniProt Gene Name
CETN2
UniProt Synonym Gene Names
CALT; CEN2
UniProt Entry Name
CETN2_HUMAN
NCBI Protein Information
centrin-2; caltractin (20kD calcium-binding protein)

NCBI Summary for CETN2

Caltractin belongs to a family of calcium-binding proteins and is a structural component of the centrosome. The high level of conservation from algae to humans and its association with the centrosome suggested that caltractin plays a fundamental role in the structure and function of the microtubule-organizing center, possibly required for the proper duplication and segregation of the centrosome. [provided by RefSeq, Jul 2008]
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UniProt Comments for CETN2

CETN2: a calcium-binding proteins and is a structural component of the centrosome. Contains 4 EF-hand calcium-binding domains. Highly conserved from algae to humans. Its association with the centrosome suggests that it plays a fundamental role in the structure and function of the microtubule-organizing center, possibly required for the proper duplication and segregation of the centrosome.

Protein type: Calcium-binding; Cell cycle regulation; DNA repair, damage

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: centriole; centrosome; intracellular; cytosol; photoreceptor connecting cilium

Molecular Function: protein binding; microtubule binding; G-protein beta/gamma-subunit binding; calcium ion binding; heterotrimeric G-protein binding

Biological Process: mitosis; cell division; nucleotide-excision repair; organelle organization and biogenesis; centriole replication; spermatogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; regulation of cytokinesis
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Research Articles on CETN2

1. co-depletion of centrin 2 and PCID2 leads to blocking rather than delaying nuclear protein export, indicating the dominance of the centrin 2 phenotype.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CETN2 cell lysate

 Reference Product  PubMed Publications
 XPC cell lysate  >22 publications with CETN2 and XPC
 RAD23B cell lysate  >5 publications with CETN2 and RAD23B
 PLK4 cell lysate  >2 publications with CETN2 and PLK4
 SFI1 cell lysate  >2 publications with CETN2 and SFI1
 USP44 cell lysate  >1 publications with CETN2 and USP44
 PCNT cell lysate  >1 publications with CETN2 and PCNT

Pathways associated with CETN2 cell lysate

 Products by Pathway  Pathway Diagram
 Anchoring Of The Basal Body To The Plasma Membrane Pathway antibodies  Anchoring Of The Basal Body To The Plasma Membrane Pathway Diagram
 Assembly Of The Primary Cilium Pathway antibodies  Assembly Of The Primary Cilium Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Centrosome Maturation Pathway antibodies  Centrosome Maturation Pathway Diagram
 G2/M Transition Pathway antibodies  G2/M Transition Pathway Diagram
 Loss Of Nlp From Mitotic Centrosomes Pathway antibodies  Loss Of Nlp From Mitotic Centrosomes Pathway Diagram
 Loss Of Proteins Required For Interphase Microtubule Organizationfrom The Centrosome Pathway antibodies  Loss Of Proteins Required For Interphase Microtubule Organizationfrom The Centrosome Pathway Diagram
 Mitotic G2-G2/M Phases Pathway antibodies  Mitotic G2-G2/M Phases Pathway Diagram
 Nucleotide Excision Repair Pathway antibodies  Nucleotide Excision Repair Pathway Diagram

Diseases associated with CETN2 cell lysate

 Disease Name  Pubmed Publications
 Congenital Abnormalities Antibodies  >6 publications with CETN2 and Congenital Abnormalities
 Precancerous Conditions Antibodies  >5 publications with CETN2 and Precancerous Conditions
 Lung Diseases Antibodies  >1 publications with CETN2 and Lung Diseases
 Nervous System Diseases Antibodies  >1 publications with CETN2 and Nervous System Diseases
 Disease Models, Animal Antibodies  >1 publications with CETN2 and Disease Models, Animal
 Death Antibodies  >1 publications with CETN2 and Death
 Immune System Diseases Antibodies  >1 publications with CETN2 and Immune System Diseases
 Adenocarcinoma Antibodies  >1 publications with CETN2 and Adenocarcinoma
 Breast Neoplasms Antibodies  >1 publications with CETN2 and Breast Neoplasms
 Brain Diseases Antibodies  >1 publications with CETN2 and Brain Diseases

Organs/Tissues associated with CETN2 cell lysate

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >3 publications with CETN2 and Eye
 Testis Antibodies  >2 publications with CETN2 and Testis
 Brain Antibodies  >2 publications with CETN2 and Brain
 Ovary Antibodies  >1 publications with CETN2 and Ovary
 Prostate Antibodies  >1 publications with CETN2 and Prostate
 Pancreas Antibodies  >1 publications with CETN2 and Pancreas
 Kidney Antibodies  >1 publications with CETN2 and Kidney
 Lung Antibodies  >1 publications with CETN2 and Lung
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