Profilin 2 (PFN2) ELISA Kit for Human

Name: Human PFN2 ELISA Kit; Profilin 2; MyBioSource
Brand: MyBioSource
SKU: MBS2707676
Price: 300 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2707676

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Profilin 2 (PFN2). Standards or samples are then added to the appropriate microtiter plate wells… More details

$300 / 24-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Profilin 2 (PFN2), ELISA Kit

Full Product Name

Human Profilin 2 (PFN2) ELISA Kit

Product Gene Name

PFN2 elisa kit

Product Synonym Names

PFL

Species Reactivity

Human

Assay Type

Double-antibody Sandwich

Samples

Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids

Detection Range

0.312-20ng/mL

Sensitivity

The minimum detectable dose of this kit is typically less than 0.137ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of Profilin 2 (PFN2).
No significant cross-reactivity or interference between Profilin 2 (PFN2) and analogues was observed.

Preparation and Storage

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

OMIM

176590

3D Structure

ModBase 3D Structure for P35080

Supply Chain Verification

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Profilin 2 (PFN2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Profilin 2 (PFN2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

ISO Certification

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Other Notes

Small volumes of PFN2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PFN2 purchase

MBS2707676 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Profilin 2 (PFN2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PFN2. The ELISA analytical biochemical technique of the MBS2707676 kit is based on PFN2 antibody-PFN2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PFN2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PFN2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PFN2. It may not necessarily be applicable to this product.

NCBI GI #

20178322

NCBI GeneID

5217

NCBI Accession #

P35080.3

UniProt Primary Accession #

P35080

UniProt Related Accession #

P35080

NCBI Official Full Name

Profilin-2

NCBI Official Synonym Full Names

profilin 2

NCBI Official Symbol

PFN2

NCBI Official Synonym Symbols

PFL; D3S1319E

UniProt Protein Name

Profilin-2

UniProt Synonym Protein Names

Profilin II

UniProt Gene Name

PFN2

UniProt Entry Name

PROF2_HUMAN

NCBI Protein Information

profilin-2

NCBI Summary for PFN2

The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. There are two alternatively spliced transcript variants encoding different isoforms described for this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for PFN2

profilin 2: Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Belongs to the profilin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 3q25.1

Cellular Component: cytoskeleton; cytoplasm; terminal button

Molecular Function: actin monomer binding; protein binding; phosphatidylinositol-4,5-bisphosphate binding; adenyl-nucleotide exchange factor activity

Biological Process: positive regulation of actin filament polymerization; positive regulation of ATPase activity; negative regulation of actin filament polymerization; positive regulation of stress fiber formation; positive regulation of actin filament bundle formation; actin cytoskeleton organization and biogenesis; positive regulation of peptidyl-serine phosphorylation

Research Articles on PFN2

1. Results suggest that PFN2 promotes the proliferation and metastasis of HNSC by activating the PI3K/Akt/beta-catenin signaling pathway.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PFN2 elisa kit

Reference Product	PubMed Publications
VASP elisa kit	>5 publications with PFN2 and VASP
ACTG1 elisa kit	>1 publications with PFN2 and ACTG1
WASL elisa kit	>1 publications with PFN2 and WASL
FHOD1 elisa kit	>1 publications with PFN2 and FHOD1

Pathways associated with PFN2 elisa kit

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Salmonella Infection Pathway antibodies	Salmonella Infection Pathway Diagram
Shigellosis Pathway antibodies	Shigellosis Pathway Diagram
Signaling By Robo Receptor Pathway antibodies	Signaling By Robo Receptor Pathway Diagram

Diseases associated with PFN2 elisa kit

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>2 publications with PFN2 and Nervous System Diseases
Lung Neoplasms Antibodies	>1 publications with PFN2 and Lung Neoplasms
Atrophy Antibodies	>1 publications with PFN2 and Atrophy
Kidney Diseases Antibodies	>1 publications with PFN2 and Kidney Diseases

Organs/Tissues associated with PFN2 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>15 publications with PFN2 and Brain
Muscle Antibodies	>4 publications with PFN2 and Muscle
Kidney Antibodies	>3 publications with PFN2 and Kidney
Testis Antibodies	>3 publications with PFN2 and Testis
Blood Antibodies	>3 publications with PFN2 and Blood
Vascular Antibodies	>2 publications with PFN2 and Vascular
Lung Antibodies	>2 publications with PFN2 and Lung
Nerve Antibodies	>2 publications with PFN2 and Nerve
Spleen Antibodies	>1 publications with PFN2 and Spleen
Liver Antibodies	>1 publications with PFN2 and Liver