RHOB ELISA Kit for Human

Name: Human RHOB ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS2504333
Price: 320 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2504333

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RHOB. Standards or samples are added to the micro ELISA plate wells and combined with… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Product Name

RHOB, ELISA Kit

Full Product Name

Human RHOB (Ras Homolog Gene Family, Memmber B) ELISA Kit

Product Gene Name

RHOB elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma and other biological fluids

Detection Range

62.5-4000pg/mL

Sensitivity

37.5pg/mL

Specificity

This kit recognizes Human RHOB in samples. No significant cross-reactivity or interference between Human RHOB and analogues was observed.

Preparation and Storage

Store at 4 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human RHOB were tested on 3 different plates, 20 replicates in each plate.

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human RHOB were tested 20 times on one plate, respectively.

Molecular Weight

22,123 Da

OMIM

165370

3D Structure

ModBase 3D Structure for P62745

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of RHOB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RHOB purchase

MBS2504333 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the RHOB, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RHOB. The ELISA analytical biochemical technique of the MBS2504333 kit is based on RHOB antibody-RHOB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RHOB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RHOB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for RHOB. It may not necessarily be applicable to this product.

NCBI GI #

4757764

NCBI GeneID

388

NCBI Accession #

NP_004031.1

UniProt Primary Accession #

P62745

UniProt Secondary Accession #

P01121; Q5U0H6; Q7RTN5; Q7RTR9; Q9CUV7; B2R692

UniProt Related Accession #

P62745

GenBank Nucleotide #

NM_004040.3

NCBI Official Full Name

rho-related GTP-binding protein RhoB

NCBI Official Synonym Full Names

ras homolog family member B

NCBI Official Symbol

RHOB

NCBI Official Synonym Symbols

ARH6; ARHB; RHOH6; MST081; MSTP081

UniProt Protein Name

Rho-related GTP-binding protein RhoB

UniProt Synonym Protein Names

Rho cDNA clone 6; h6

Protein Family

Rho-related GTP-binding protein

UniProt Gene Name

RHOB

UniProt Synonym Gene Names

ARH6; ARHB; h6

UniProt Entry Name

RHOB_HUMAN

NCBI Protein Information

rho-related GTP-binding protein RhoB; Aplysia RAS-related homolog 6; h6; oncogene RHO H6; ras homolog gene family, member B; rho cDNA clone 6

UniProt Comments for RHOB

RHOB: a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Controls the reorganization of actins into podosomes. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders.

Protein type: G protein, monomeric; Motility/polarity/chemotaxis; Tumor suppressor; G protein, monomeric, Rho; G protein

Chromosomal Location of Human Ortholog: 2p24

Cellular Component: focal adhesion; late endosome membrane; early endosome; plasma membrane; endosome membrane; cytosol; nucleus; cleavage furrow

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding

Biological Process: axon guidance; platelet activation; transformed cell apoptosis; positive regulation of apoptosis; metabolic process; apoptosis; cytokinesis; Rho protein signal transduction; negative regulation of cell cycle; intracellular protein transport; positive regulation of angiogenesis; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; endosome to lysosome transport; angiogenesis; cell adhesion; blood coagulation

Research Articles on RHOB

1. RHOB belongs to a novel class of "genes of recurrence" that have a dual role in metastasis and treatment resistance.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with RHOB elisa kit

Reference Product	PubMed Publications
ARHGEF1 elisa kit	>32 publications with RHOB and ARHGEF1
LIMK1 elisa kit	>11 publications with RHOB and LIMK1
ERBB2 elisa kit	>8 publications with RHOB and ERBB2
ARHGDIA elisa kit	>5 publications with RHOB and ARHGDIA
ARHGEF2 elisa kit	>3 publications with RHOB and ARHGEF2
GRLF1 elisa kit	>1 publications with RHOB and GRLF1
ECT2 elisa kit	>1 publications with RHOB and ECT2
ARHGDIG elisa kit	>1 publications with RHOB and ARHGDIG

Pathways associated with RHOB elisa kit

Products by Pathway	Pathway Diagram
Androgen Receptor Signaling Pathway antibodies	Androgen Receptor Signaling Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
G Alpha (12/13) Signalling Events Pathway antibodies	G Alpha (12/13) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPVI-mediated Activation Cascade Pathway antibodies	GPVI-mediated Activation Cascade Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Neural Crest Differentiation Pathway antibodies	Neural Crest Differentiation Pathway Diagram
Platelet Activation, Signaling And Aggregation Pathway antibodies	Platelet Activation, Signaling And Aggregation Pathway Diagram

Diseases associated with RHOB elisa kit

Disease Name	Pubmed Publications
Cell Transformation, Neoplastic Antibodies	>37 publications with RHOB and Cell Transformation, Neoplastic
Lung Neoplasms Antibodies	>22 publications with RHOB and Lung Neoplasms
Neoplasm Metastasis Antibodies	>22 publications with RHOB and Neoplasm Metastasis
Nervous System Diseases Antibodies	>19 publications with RHOB and Nervous System Diseases
Neoplasms, Experimental Antibodies	>14 publications with RHOB and Neoplasms, Experimental
Disease Models, Animal Antibodies	>14 publications with RHOB and Disease Models, Animal
Brain Diseases Antibodies	>14 publications with RHOB and Brain Diseases
Inflammation Antibodies	>10 publications with RHOB and Inflammation
Kidney Diseases Antibodies	>9 publications with RHOB and Kidney Diseases
Necrosis Antibodies	>9 publications with RHOB and Necrosis

Organs/Tissues associated with RHOB elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>68 publications with RHOB and Brain
Blood Antibodies	>59 publications with RHOB and Blood
Vascular Antibodies	>46 publications with RHOB and Vascular
Lung Antibodies	>42 publications with RHOB and Lung
Kidney Antibodies	>31 publications with RHOB and Kidney
Liver Antibodies	>25 publications with RHOB and Liver
Bone Antibodies	>24 publications with RHOB and Bone
Nerve Antibodies	>21 publications with RHOB and Nerve
Heart Antibodies	>17 publications with RHOB and Heart
Prostate Antibodies	>13 publications with RHOB and Prostate