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ADD1 Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS857251

MBS857251 is an antibody targeting ADD1. Also known as ADD1 (Phospho-Ser726) Antibody. Reactive in Human, Mouse, Rat. More details

$295 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ADD1, Antibody
Full Product Name
ADD1 (Phospho-Ser726) Antibody
Product Gene Name
Species Reactivity
Human, Mouse, Rat
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificity
ADD1 (Phospho-Ser726) antibody detects endogenous levels of ADD1 only when phosphorylated at serine 726.
Preparation and Storage
Store at-20 degree C for 1 year
Molecular Weight
73,333 Da
OMIM
102680
3D Structure
ModBase 3D Structure for P35611
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human ADD1 around the phosphorylation site of serine 726 (T-P-S P-F-L).
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-ADD1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-ADD1 antibody
WB: 1:500~1:1000
IHC: 1:50~1:100

Immunohistochemistry (IHC) of anti-ADD1 antibody

P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ADD1 (Phospho-Ser726) antibody.
Anti-ADD1 Antibody – Cat# MBS857251

Western Blot (WB) of anti-ADD1 antibody

Western blot analysis of extract from HT-29 cells untreated or treated with Doxorubicin (1mM, 30min), using ADD1 (Ab-726) antibody and ADD1 (Phospho-Ser726) antibody.
Anti-ADD1 Antibody, Western Blot (WB) – Cat# MBS857251

Product References and Citations for anti-ADD1 antibody

Pariser H, et al. Proc Natl Acad Sci USA 2005 Aug 30; 102(35): 12407-12 Tamaru S, et al. Biochem Biophys Res Commun 2005 Jul 01; 332(2): 347-51 Barkalow KL, et al. J Cell Biol 2003 May 12; 161(3): 557-70.

Other Notes

Small volumes of anti-ADD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ADD1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
alpha-adducin isoform a
NCBI Official Synonym Full Names
adducin 1
NCBI Official Symbol
ADD1
NCBI Official Synonym Symbols
ADDA
UniProt Protein Name
Alpha-adducin
UniProt Synonym Protein Names
Erythrocyte adducin subunit alpha
Protein Family
UniProt Gene Name
ADD1
UniProt Synonym Gene Names
ADDA
NCBI Protein Information
alpha-adducin

NCBI Summary for ADD1

Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, and gamma). Adducin acts as a heterodimer of the related alpha, beta, or gamma subunits. The protein encoded by this gene represents the alpha subunit. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. [provided by RefSeq, Aug 2017]
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UniProt Comments for ADD1

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ADD1 antibody

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