ADRBK2 siRNA – Beta-Adrenergic Receptor Kinase 2 (Human)

Name: ADRBK2 siRNA; Beta-Adrenergic Receptor Kinase 2; Human; MyBioSource
Brand: MyBioSource
SKU: MBS829542
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS829542

MBS829542 is an siRNA targeting ADRBK2. Also known as ADRBK2 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit ADRBK2 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ADRBK2, siRNA

Full Product Name

ADRBK2 siRNA (Human)

Product Gene Name

ADRBK2 sirna

Product Synonym Names

BARK2; GRK3; Beta-adrenergic receptor kinase 2; Beta-ARK-2; G-protein-coupled receptor kinase 3

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

ADRBK2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

79,710 Da

OMIM

109636

3D Structure

ModBase 3D Structure for P35626

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ADRBK2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for ADRBK2 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of ADRBK2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ADRBK2. It may not necessarily be applicable to this product.

NCBI GI #

148539879

NCBI GeneID

157

NCBI Accession #

NP_005151.2

UniProt Primary Accession #

P35626

UniProt Secondary Accession #

Q9UGW9

UniProt Related Accession #

P35626

GenBank Nucleotide #

NM_005160.3

NCBI Official Full Name

beta-adrenergic receptor kinase 2

NCBI Official Synonym Full Names

adrenergic, beta, receptor kinase 2

NCBI Official Symbol

ADRBK2

NCBI Official Synonym Symbols

GRK3; BARK2

UniProt Protein Name

Beta-adrenergic receptor kinase 2

UniProt Synonym Protein Names

G-protein-coupled receptor kinase 3

Protein Family

Beta-adrenergic receptor kinase

UniProt Gene Name

ADRBK2

UniProt Synonym Gene Names

BARK2; GRK3; Beta-ARK-2

UniProt Entry Name

ARBK2_HUMAN

NCBI Protein Information

beta-adrenergic receptor kinase 2

NCBI Summary for ADRBK2

The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function. [provided by RefSeq, Jul 2008]

UniProt Comments for ADRBK2

GRK3: Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Protein type: Kinase, protein; EC 2.7.11.15; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; GRK family; BARK subfamily

Chromosomal Location of Human Ortholog: 22q12.1

Molecular Function: G-protein coupled receptor kinase activity; beta-adrenergic receptor kinase activity; ATP binding; protein kinase activity

Biological Process: receptor internalization; signal transduction; protein amino acid phosphorylation

Research Articles on ADRBK2

1. data are consistent with the possibility that oncogenes can induce cellular stiffness via an HDAC6-induced reorganization of the vimentin intermediate filament network

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ADRBK2 sirna

Reference Product	PubMed Publications
CXCR4 sirna	>8 publications with ADRBK2 and CXCR4
TBXA2R sirna	>2 publications with ADRBK2 and TBXA2R
CCR5 sirna	>1 publications with ADRBK2 and CCR5
CCR4 sirna	>1 publications with ADRBK2 and CCR4
ARRB2 sirna	>1 publications with ADRBK2 and ARRB2

Pathways associated with ADRBK2 sirna

Products by Pathway	Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Corticotropin-releasing Hormone Pathway antibodies	Corticotropin-releasing Hormone Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram
Glutamatergic Synapse Pathway antibodies	Glutamatergic Synapse Pathway Diagram
Glutamatergic Synapse Pathway antibodies	Glutamatergic Synapse Pathway Diagram
IL-4 Signaling Pathway antibodies	IL-4 Signaling Pathway Diagram
Morphine Addiction Pathway antibodies	Morphine Addiction Pathway Diagram
Morphine Addiction Pathway antibodies	Morphine Addiction Pathway Diagram

Diseases associated with ADRBK2 sirna

Disease Name	Pubmed Publications
Bipolar Disorder Antibodies	>14 publications with ADRBK2 and Bipolar Disorder
Heart Diseases Antibodies	>8 publications with ADRBK2 and Heart Diseases
Hypertension Antibodies	>7 publications with ADRBK2 and Hypertension
Brain Diseases Antibodies	>6 publications with ADRBK2 and Brain Diseases
Hypertrophy Antibodies	>4 publications with ADRBK2 and Hypertrophy
Inflammation Antibodies	>4 publications with ADRBK2 and Inflammation
Death Antibodies	>3 publications with ADRBK2 and Death
Asthma Antibodies	>3 publications with ADRBK2 and Asthma
Respiratory Tract Diseases Antibodies	>2 publications with ADRBK2 and Respiratory Tract Diseases
Movement Disorders Antibodies	>2 publications with ADRBK2 and Movement Disorders

Organs/Tissues associated with ADRBK2 sirna

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>40 publications with ADRBK2 and Brain
Muscle Antibodies	>25 publications with ADRBK2 and Muscle
Blood Antibodies	>22 publications with ADRBK2 and Blood
Heart Antibodies	>20 publications with ADRBK2 and Heart
Kidney Antibodies	>10 publications with ADRBK2 and Kidney
Eye Antibodies	>9 publications with ADRBK2 and Eye
Vascular Antibodies	>9 publications with ADRBK2 and Vascular
Nerve Antibodies	>8 publications with ADRBK2 and Nerve
Embryonic Tissue Antibodies	>4 publications with ADRBK2 and Embryonic Tissue
Ovary Antibodies	>3 publications with ADRBK2 and Ovary