AKAP14 siRNA – A-Kinase Anchor Protein 14 Isoform B (Human)

Name: AKAP14 siRNA; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8229246
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8229246

MBS8229246 is an siRNA targeting AKAP14. Also known as AKAP14 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit AKAP14 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

AKAP14, siRNA

Full Product Name

AKAP14 siRNA (Human)

Product Gene Name

AKAP14 sirna

Product Synonym Names

AKAP28; A-kinase anchor protein 14; AKAP-14; A-kinase anchor protein 28 kDa; AKAP 28; Protein kinase A-anchoring protein 14; PRKA14

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

AKAP14 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

10,187 Da

OMIM

300462

3D Structure

ModBase 3D Structure for Q86UN6

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AKAP14 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for AKAP14 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of AKAP14 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AKAP14. It may not necessarily be applicable to this product.

NCBI GI #

56682938

NCBI GeneID

158798

NCBI Accession #

NP_001008534.1

UniProt Primary Accession #

Q86UN6

UniProt Secondary Accession #

Q86UN4; Q86UN5; A6NNZ0

UniProt Related Accession #

Q86UN6

GenBank Nucleotide #

NM_001008534.1

NCBI Official Full Name

A-kinase anchor protein 14 isoform b

NCBI Official Synonym Full Names

A kinase (PRKA) anchor protein 14

NCBI Official Symbol

AKAP14

NCBI Official Synonym Symbols

AKAP28; PRKA14

UniProt Protein Name

A-kinase anchor protein 14

UniProt Synonym Protein Names

A-kinase anchor protein 28 kDa; AKAP 28; Protein kinase A-anchoring protein 14; PRKA14

Protein Family

A-kinase anchor protein

UniProt Gene Name

AKAP14

UniProt Synonym Gene Names

AKAP28; AKAP-14; AKAP 28; PRKA14

UniProt Entry Name

AKA28_HUMAN

NCBI Protein Information

A-kinase anchor protein 14

NCBI Summary for AKAP14

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The protein anchors PKA in ciliary axonemes and, in this way, may play a role in regulating ciliary beat frequency. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

UniProt Comments for AKAP14

AKAP14: Binds to type II regulatory subunits of protein kinase A and anchors/targets them. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: Xq24

Cellular Component: cytoplasm

Molecular Function: protein kinase A binding

Biological Process: spermatogenesis

Research Articles on AKAP14

1. AKAP28 likely plays a role in the signaling necessary for efficient modulation of ciliary beat frequency

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with AKAP14 sirna

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>1 publications with AKAP14 and Lung
Testis Antibodies	>1 publications with AKAP14 and Testis