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AMD1 siRNA – S-Adenosylmethionine Decarboxylase Proenzyme Isoform 1 (Human)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8221092

MBS8221092 is an siRNA targeting AMD1. Also known as AMD1 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit AMD1 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
AMD1, siRNA
Full Product Name
AMD1 siRNA (Human)
Product Gene Name
AMD1 sirna
Product Synonym Names
AMD; S-adenosylmethionine decarboxylase proenzyme; AdoMetDC; SAMDC
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
AMD1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
21,301 Da
OMIM
180980
3D Structure
ModBase 3D Structure for P17707
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AMD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for AMD1 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of AMD1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AMD1. It may not necessarily be applicable to this product.

NCBI GI #
74275346
NCBI GeneID
262
NCBI Accession #
NP_001625.2
UniProt Primary Accession #
P17707
UniProt Secondary Accession #
Q5VXN4; Q5VXN6; Q9BWK4; E1P5F7
UniProt Related Accession #
P17707
GenBank Nucleotide #
NM_001634.5
NCBI Official Full Name
S-adenosylmethionine decarboxylase proenzyme isoform 1
NCBI Official Synonym Full Names
adenosylmethionine decarboxylase 1
NCBI Official Symbol
AMD1
NCBI Official Synonym Symbols
AMD; SAMDC; ADOMETDC
UniProt Protein Name
S-adenosylmethionine decarboxylase proenzyme
Protein Family
S-adenosylmethionine decarboxylase proenzyme
UniProt Gene Name
AMD1
UniProt Synonym Gene Names
AMD; AdoMetDC; SAMDC
UniProt Entry Name
DCAM_HUMAN
NCBI Protein Information
S-adenosylmethionine decarboxylase proenzyme

NCBI Summary for AMD1

This gene encodes an important intermediate enzyme in polyamine biosynthesis. The polyamines spermine, spermidine, and putrescine are low-molecular-weight aliphatic amines essential for cellular proliferation and tumor promotion. Multiple alternatively spliced transcript variants have been identified. Pseudogenes of this gene are found on chromosomes 5, 6, 10, X and Y. [provided by RefSeq, Dec 2013]
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UniProt Comments for AMD1

AMD1: an important intermediate enzyme in polyamine biosynthesis. The polyamines spermine, spermidine, and putrescine are low-molecular-weight aliphatic amines essential for cellular proliferation and tumor promotion. Two alternatively spliced transcript variants that encode different proteins have been identified. Pseudogenes of this gene are found on chromosomes 5, 6, 10, X and Y.[provided by RefSeq, Jul 2010]

Protein type: Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - arginine and proline; Lyase; EC 4.1.1.50

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cytosol

Molecular Function: adenosylmethionine decarboxylase activity

Biological Process: S-adenosylmethioninamine biosynthetic process; spermidine biosynthetic process; spermine biosynthetic process; polyamine metabolic process
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Research Articles on AMD1

1. Gene expression studies have identified altered expression of AMD1 in suicide completers with a history of mood disorders.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with AMD1 sirna

 Reference Product  PubMed Publications
 ODC1 sirna  >7 publications with AMD1 and ODC1
 SMS sirna  >1 publications with AMD1 and SMS
 SRM sirna  >1 publications with AMD1 and SRM

Pathways associated with AMD1 sirna

 Products by Pathway  Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 Metabolism Of Polyamines Pathway antibodies  Metabolism Of Polyamines Pathway Diagram
 Methionine Salvage Pathway antibodies  Methionine Salvage Pathway Diagram
 Methionine Salvage Pathway antibodies  Methionine Salvage Pathway Diagram

Diseases associated with AMD1 sirna

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >2 publications with AMD1 and Disease Models, Animal
 Oligospermia Antibodies  >1 publications with AMD1 and Oligospermia
 Hypertrophy Antibodies  >1 publications with AMD1 and Hypertrophy
 Cardiovascular Diseases Antibodies  >1 publications with AMD1 and Cardiovascular Diseases

Organs/Tissues associated with AMD1 sirna

 Organ/Tissue Name  Pubmed Publications
 Ovary Antibodies  >2 publications with AMD1 and Ovary
 Muscle Antibodies  >2 publications with AMD1 and Muscle
 Blood Antibodies  >2 publications with AMD1 and Blood
 Uterus Antibodies  >2 publications with AMD1 and Uterus
 Brain Antibodies  >2 publications with AMD1 and Brain
 Eye Antibodies  >1 publications with AMD1 and Eye
 Embryonic Tissue Antibodies  >1 publications with AMD1 and Embryonic Tissue
 Prostate Antibodies  >1 publications with AMD1 and Prostate
 Testis Antibodies  >1 publications with AMD1 and Testis
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