AMOTL2 siRNA – Angiomotin-Like Protein 2 Isoform 1 (Human)

Name: AMOTL2 siRNA; Angiomotin-Like Protein 2 Isoform 1; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8221692
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8221692

MBS8221692 is an siRNA targeting AMOTL2. Also known as AMOTL2 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit AMOTL2 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

AMOTL2, siRNA

Full Product Name

AMOTL2 siRNA (Human)

Product Gene Name

AMOTL2 sirna

Product Synonym Names

KIAA0989; Angiomotin-like protein 2; Leman coiled-coil protein; LCCP

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

AMOTL2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

91,817 Da

OMIM

614658

3D Structure

ModBase 3D Structure for Q9Y2J4

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AMOTL2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for AMOTL2 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of AMOTL2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AMOTL2. It may not necessarily be applicable to this product.

NCBI GI #

522838240

NCBI GeneID

51421

NCBI Accession #

NP_001265612.1

UniProt Primary Accession #

Q9Y2J4

UniProt Secondary Accession #

Q53EP1; Q96F99; Q9UKB4; A8K6F1; B7Z5Q1; E9PHW3

UniProt Related Accession #

Q9Y2J4

GenBank Nucleotide #

NM_001278683.1

NCBI Official Full Name

angiomotin-like protein 2 isoform 1

NCBI Official Synonym Full Names

angiomotin like 2

NCBI Official Symbol

AMOTL2

NCBI Official Synonym Symbols

LCCP

UniProt Protein Name

Angiomotin-like protein 2

UniProt Synonym Protein Names

Leman coiled-coil protein; LCCP

Protein Family

Angiomotin-like protein

UniProt Gene Name

AMOTL2

UniProt Synonym Gene Names

KIAA0989; LCCP

UniProt Entry Name

AMOL2_HUMAN

NCBI Protein Information

angiomotin-like protein 2

NCBI Summary for AMOTL2

Angiomotin is a protein that binds angiostatin, a circulating inhibitor of the formation of new blood vessels (angiogenesis). Angiomotin mediates angiostatin inhibition of endothelial cell migration and tube formation in vitro. The protein encoded by this gene is related to angiomotin and is a member of the motin protein family. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

UniProt Comments for AMOTL2

AMOTL2: Regulates the translocation of phosphorylated SRC to peripheral cell-matrix adhesion sites. Required for proper architecture of actin filaments. Inhibits the Wnt/beta-catenin signaling pathway, probably by recruiting CTNNB1 to recycling endosomes and hence preventing its translocation to the nucleus. Participates in angiogenesis. May play a role in the polarity, proliferation and migration of endothelial cells. Selectively promotes FGF-induced MAPK activation through SRC. Belongs to the angiomotin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion

Chromosomal Location of Human Ortholog: 3q21-q22

Cellular Component: recycling endosome; tight junction; apical plasma membrane; cytoplasmic vesicle; cytosol

Molecular Function: identical protein binding; protein binding

Biological Process: Wnt receptor signaling pathway

Research Articles on AMOTL2

1. Scaffold proteins angiomotin (Amot) and angiomotin-related AmotL1 and AmotL2 were recently identified as negative regulators of YAP and TAZ by preventing their nuclear translocation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with AMOTL2 sirna

Reference Product	PubMed Publications
AMOTL1 sirna	>11 publications with AMOTL2 and AMOTL1
LATS2 sirna	>4 publications with AMOTL2 and LATS2
YAP1 sirna	>4 publications with AMOTL2 and YAP1
LATS1 sirna	>2 publications with AMOTL2 and LATS1

Pathways associated with AMOTL2 sirna

Products by Pathway	Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By Hippo Pathway antibodies	Signaling By Hippo Pathway Diagram

Diseases associated with AMOTL2 sirna

Disease Name	Pubmed Publications
Liver Neoplasms Antibodies	>1 publications with AMOTL2 and Liver Neoplasms
Breast Neoplasms Antibodies	>1 publications with AMOTL2 and Breast Neoplasms

Organs/Tissues associated with AMOTL2 sirna

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>3 publications with AMOTL2 and Kidney
Muscle Antibodies	>2 publications with AMOTL2 and Muscle
Thyroid Antibodies	>1 publications with AMOTL2 and Thyroid
Ovary Antibodies	>1 publications with AMOTL2 and Ovary
Eye Antibodies	>1 publications with AMOTL2 and Eye
Liver Antibodies	>1 publications with AMOTL2 and Liver
Heart Antibodies	>1 publications with AMOTL2 and Heart
Lung Antibodies	>1 publications with AMOTL2 and Lung
Uterus Antibodies	>1 publications with AMOTL2 and Uterus
Bone Antibodies	>1 publications with AMOTL2 and Bone