B3GAT1 siRNA – Galactosylgalactosylxylosylprotein 3-Beta-Glucuronosyltransferase 1 (Human)

Name: B3GAT1 siRNA; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8223157
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8223157

MBS8223157 is an siRNA targeting B3GAT1. Also known as B3GAT1 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit B3GAT1 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

B3GAT1, siRNA

Full Product Name

B3GAT1 siRNA (Human)

Product Gene Name

B3GAT1 sirna

Product Synonym Names

GLCATP; Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; Beta-1.3-glucuronyltransferase 1; Glucuronosyltransferase P; GlcAT-P; UDP-GlcUA:glycoprotein beta-1.3-glucuronyltransferase; GlcUAT-P

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

B3GAT1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

38,256 Da

OMIM

151290

3D Structure

ModBase 3D Structure for Q9P2W7

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human B3GAT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for B3GAT1 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of B3GAT1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for B3GAT1. It may not necessarily be applicable to this product.

NCBI GI #

77695914

NCBI GeneID

27087

NCBI Accession #

NP_061114.2

UniProt Primary Accession #

Q9P2W7

UniProt Secondary Accession #

Q96FS7

UniProt Related Accession #

Q9P2W7

GenBank Nucleotide #

NM_018644.3

NCBI Official Full Name

galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1

NCBI Official Synonym Full Names

beta-1,3-glucuronyltransferase 1

NCBI Official Symbol

B3GAT1

NCBI Official Synonym Symbols

NK1; CD57; HNK1; LEU7; NK-1; GLCATP; GLCUATP

UniProt Protein Name

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1

UniProt Synonym Protein Names

Beta-1,3-glucuronyltransferase 1; Glucuronosyltransferase P; GlcAT-P; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase; GlcUAT-P

Protein Family

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase

UniProt Gene Name

B3GAT1

UniProt Synonym Gene Names

GLCATP; GlcAT-P; GlcUAT-P

UniProt Entry Name

B3GA1_HUMAN

NCBI Protein Information

galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1

NCBI Summary for B3GAT1

The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]

UniProt Comments for B3GAT1

B3GAT1: Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo- orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl- sphingomyelin was the most effective, followed by palmitoyl- sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group. Belongs to the glycosyltransferase 43 family.

Protein type: Transferase; EC 2.4.1.135; Membrane protein, integral; Glycan Metabolism - heparan sulfate biosynthesis; Glycan Metabolism - chondroitin sulfate biosynthesis

Chromosomal Location of Human Ortholog: 11q25

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: metal ion binding; UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity; galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity

Biological Process: chondroitin sulfate metabolic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; protein amino acid glycosylation; pathogenesis

Research Articles on B3GAT1

1. glioblastoma stem cells are killed by CD133-specific CAR T cells but induce the T cell aging marker CD57

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with B3GAT1 sirna

Reference Product	PubMed Publications
B4GALT7 sirna	>1 publications with B3GAT1 and B4GALT7
CHST10 sirna	>1 publications with B3GAT1 and CHST10

Pathways associated with B3GAT1 sirna

Products by Pathway	Pathway Diagram
A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway antibodies	A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway Diagram
Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway antibodies	Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway Diagram
Glycosaminoglycan Metabolism Pathway antibodies	Glycosaminoglycan Metabolism Pathway Diagram
Heparan Sulfate/heparin (HS-GAG) Metabolism Pathway antibodies	Heparan Sulfate/heparin (HS-GAG) Metabolism Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Carbohydrates Pathway antibodies	Metabolism Of Carbohydrates Pathway Diagram
Other Types Of O-glycan Biosynthesis Pathway antibodies	Other Types Of O-glycan Biosynthesis Pathway Diagram
Other Types Of O-glycan Biosynthesis Pathway antibodies	Other Types Of O-glycan Biosynthesis Pathway Diagram
Chondroitin Sulfate Biosynthesis Pathway antibodies	Chondroitin Sulfate Biosynthesis Pathway Diagram
Chondroitin Sulfate Biosynthesis Pathway antibodies	Chondroitin Sulfate Biosynthesis Pathway Diagram

Diseases associated with B3GAT1 sirna

Disease Name	Pubmed Publications
Atrophy Antibodies	>1 publications with B3GAT1 and Atrophy
Prenatal Injuries Antibodies	>1 publications with B3GAT1 and Prenatal Injuries
Necrosis Antibodies	>1 publications with B3GAT1 and Necrosis
Prenatal Exposure Delayed Effects Antibodies	>1 publications with B3GAT1 and Prenatal Exposure Delayed Effects

Organs/Tissues associated with B3GAT1 sirna

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>5 publications with B3GAT1 and Brain
Nerve Antibodies	>4 publications with B3GAT1 and Nerve
Blood Antibodies	>2 publications with B3GAT1 and Blood