BHLHE23 siRNA – Class E Basic Helix-Loop-Helix Protein 23 (Human)

Name: BHLHE23 siRNA; Human; MyBioSource
Brand: MyBioSource
SKU: MBS829664
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS829664

MBS829664 is an siRNA targeting BHLHE23. Also known as BHLHE23 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit BHLHE23 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

BHLHE23, siRNA

Full Product Name

BHLHE23 siRNA (Human)

Product Gene Name

BHLHE23 sirna

Product Synonym Names

BHLHB4; Class E basic helix-loop-helix protein 23; bHLHe23; Class B basic helix-loop-helix protein 4; bHLHb4

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

BHLHE23 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

23,665 Da

OMIM

609331

3D Structure

ModBase 3D Structure for Q8NDY6

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human BHLHE23 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for BHLHE23 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of BHLHE23 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for BHLHE23. It may not necessarily be applicable to this product.

NCBI GI #

506326971

NCBI GeneID

128408

NCBI Accession #

NP_542173.2

UniProt Primary Accession #

Q8NDY6

UniProt Secondary Accession #

B2RP69

UniProt Related Accession #

Q8NDY6

GenBank Nucleotide #

NM_080606.3

NCBI Official Full Name

class E basic helix-loop-helix protein 23

NCBI Official Synonym Full Names

basic helix-loop-helix family, member e23

NCBI Official Symbol

BHLHE23

NCBI Official Synonym Symbols

BETA4; BHLHB4; bA305P22.3

UniProt Protein Name

Class E basic helix-loop-helix protein 23

UniProt Synonym Protein Names

Class B basic helix-loop-helix protein 4; bHLHb4

Protein Family

Class E basic helix-loop-helix protein

UniProt Gene Name

BHLHE23

UniProt Synonym Gene Names

BHLHB4; bHLHe23; bHLHb4

UniProt Entry Name

BHE23_HUMAN

NCBI Protein Information

class E basic helix-loop-helix protein 23

NCBI Summary for BHLHE23

This gene encodes a member of the basic helix-loop-helix transcription factor family. Members of this family contain two highly conserved and functionally distinct domains: the basic domain targets sequence-specific DNA binding, while the helix-loop-helix domain facilitates protein interaction. Studies of a related gene in mouse suggest that the encoded protein may function as a transcriptional repressor in the pancreas and brain, and that it is required for normal retinal function. [provided by RefSeq, May 2013]

UniProt Comments for BHLHE23

BHLHE23: May function as transcriptional repressor. May modulate the expression of genes required for the differentiation and/or maintenance of pancreatic and neuronal cell types. May be important for rod bipolar cell maturation.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: nucleus

Molecular Function: protein dimerization activity; DNA binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent

Research Articles on BHLHE23

1. Knockout mouse retinal dysfunction resembles human congenital stationary night blindness.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.