BRSK2 siRNA – Serine/Threonine-Protein Kinase BRSK2 Isoform 1 (Human)

Name: BRSK2 siRNA; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8224328
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8224328

MBS8224328 is an siRNA targeting BRSK2. Also known as BRSK2 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit BRSK2 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

BRSK2, siRNA

Full Product Name

BRSK2 siRNA (Human)

Product Gene Name

BRSK2 sirna

Product Synonym Names

C11orf7; PEN11B; SADA; STK29; Serine/threonine-protein kinase BRSK2; Brain-selective kinase 2; Brain-specific serine/threonine-protein kinase 2; BR serine/threonine-protein kinase 2; Serine/threonine-protein kinase 29; Serine/threonine-protein kinase SAD-A

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

BRSK2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

68,740 Da

OMIM

609236

3D Structure

ModBase 3D Structure for Q8IWQ3

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human BRSK2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for BRSK2 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of BRSK2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for BRSK2. It may not necessarily be applicable to this product.

NCBI GI #

375298744

NCBI GeneID

9024

NCBI Accession #

NP_001243556.1

UniProt Primary Accession #

Q8IWQ3

UniProt Secondary Accession #

O60843; O95099; Q5J5B4; Q6ZMQ4; Q8TB60; B3KVE9; E9PLM7

UniProt Related Accession #

Q8IWQ3

GenBank Nucleotide #

NM_001256627.1

NCBI Official Full Name

serine/threonine-protein kinase BRSK2 isoform 1

NCBI Official Synonym Full Names

BR serine/threonine kinase 2

NCBI Official Symbol

BRSK2

NCBI Official Synonym Symbols

SAD1; SADA; STK29; PEN11B; C11orf7

UniProt Protein Name

Serine/threonine-protein kinase BRSK2

UniProt Synonym Protein Names

Brain-selective kinase 2 (EC:2.7.11.26); Brain-specific serine/threonine-protein kinase 2; BR serine/threonine-protein kinase 2; Serine/threonine-protein kinase 29; Serine/threonine-protein kinase SAD-A

Protein Family

Serine/threonine-protein kinase

UniProt Gene Name

BRSK2

UniProt Synonym Gene Names

C11orf7; PEN11B; SADA; STK29; BR serine/threonine-protein kinase 2

UniProt Entry Name

BRSK2_HUMAN

NCBI Protein Information

serine/threonine-protein kinase BRSK2

UniProt Comments for BRSK2

BRSK2: a serine/threonine protein kinase of the CAMK group. Closely related to AMPK. Activated by the kinase LKB1. BRSK1 and BRSK2 are required for nueronal polarization. Expressed in the brain and at low levels in the testis. Four alternatively spliced isoforms have been described.

Protein type: EC 2.7.11.1; Protein kinase, CAMK; EC 2.7.11.26; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CAMK group; CAMKL family; BRSK subfamily

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: centrosome; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm

Molecular Function: protein serine/threonine kinase activity; tau-protein kinase activity; magnesium ion binding; protein kinase binding; ATP binding

Biological Process: neuron differentiation; mitosis; peptidyl-serine phosphorylation; exocytosis; axonogenesis; establishment of cell polarity; cell division; actin cytoskeleton reorganization; G2/M transition of mitotic cell cycle; protein amino acid phosphorylation

Research Articles on BRSK2

1. SAD-A and AMPK kinases are regulators of mTORC1 signaling in the pancreatic beta-cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with BRSK2 sirna

Reference Product	PubMed Publications
STK11 sirna	>7 publications with BRSK2 and STK11
WEE1 sirna	>1 publications with BRSK2 and WEE1

Pathways associated with BRSK2 sirna

Products by Pathway	Pathway Diagram
LKB1 Signaling Events Pathway antibodies	LKB1 Signaling Events Pathway Diagram

Diseases associated with BRSK2 sirna

Disease Name	Pubmed Publications
Neoplasm Metastasis Antibodies	>1 publications with BRSK2 and Neoplasm Metastasis

Organs/Tissues associated with BRSK2 sirna

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>19 publications with BRSK2 and Brain
Liver Antibodies	>2 publications with BRSK2 and Liver
Lung Antibodies	>1 publications with BRSK2 and Lung
Testis Antibodies	>1 publications with BRSK2 and Testis