CCNB3 siRNA – G2/Mitotic-Specific Cyclin-B3 Isoform 3 (Human)

Name: CCNB3 siRNA; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8240243
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8240243

MBS8240243 is an siRNA targeting CCNB3. Also known as CCNB3 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit CCNB3 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CCNB3, siRNA

Full Product Name

CCNB3 siRNA (Human)

Product Gene Name

CCNB3 sirna

Product Synonym Names

CYCB3; G2/mitotic-specific cyclin-B3

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

CCNB3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

12,195 Da

OMIM

300456

3D Structure

ModBase 3D Structure for Q8WWL7

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CCNB3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for CCNB3 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of CCNB3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CCNB3. It may not necessarily be applicable to this product.

NCBI GI #

90669307

NCBI GeneID

85417

NCBI Accession #

NP_149020.2

UniProt Primary Accession #

Q8WWL7

UniProt Secondary Accession #

Q96SB5; Q96SB6; Q96SB7; Q9NT38; B1AQI5; B1AQI6

UniProt Related Accession #

Q8WWL7

GenBank Nucleotide #

NM_033031.2

NCBI Official Full Name

G2/mitotic-specific cyclin-B3 isoform 3

NCBI Official Synonym Full Names

cyclin B3

NCBI Official Symbol

CCNB3

UniProt Protein Name

G2/mitotic-specific cyclin-B3

UniProt Gene Name

CCNB3

UniProt Synonym Gene Names

CYCB3

UniProt Entry Name

CCNB3_HUMAN

NCBI Protein Information

G2/mitotic-specific cyclin-B3

NCBI Summary for CCNB3

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as positive regulators of cyclin-dependent kinases (CDKs), and thereby play an essential role in the control of the cell cycle. Different cyclins exhibit distinct expression and degradation patterns, which contribute to the temporal coordination of each mitotic event. Studies of similar genes in chicken and drosophila suggest that this cyclin may associate with CDC2 and CDK2 kinases, and may be required for proper spindle reorganization and restoration of the interphase nucleus. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]

UniProt Comments for CCNB3

CCNB3: Cyclins are positive regulatory subunits of the cyclin- dependent kinases (CDKs), and thereby play an essential role in the control of the cell cycle, notably via their destruction during cell division. Its tissue specificity suggest that it may be required during early meiotic prophase I. Belongs to the cyclin family. Cyclin AB subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: Xp11

Cellular Component: nucleoplasm

Molecular Function: protein binding; protein kinase binding

Biological Process: meiotic cell cycle; cell division; regulation of G2/M transition of mitotic cell cycle; regulation of cyclin-dependent protein kinase activity

Research Articles on CCNB3

1. Study provides a detailed description of the histologic spectrum, immunohistochemical features, and clinical characteristic of BCOR-CCNB3 sarcoma justifying distinction from Ewing sarcoma with its typical EWS/FUS-ETS translocations.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CCNB3 sirna

Reference Product	PubMed Publications
CCNB1 sirna	>4 publications with CCNB3 and CCNB1
CCNB2 sirna	>3 publications with CCNB3 and CCNB2
CDK2 sirna	>3 publications with CCNB3 and CDK2

Pathways associated with CCNB3 sirna

Products by Pathway	Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle - G2/M Transition Pathway antibodies	Cell Cycle - G2/M Transition Pathway Diagram
Cell Cycle - G2/M Transition Pathway antibodies	Cell Cycle - G2/M Transition Pathway Diagram
DNA Damage Response Pathway antibodies	DNA Damage Response Pathway Diagram
FoxO Signaling Pathway antibodies	FoxO Signaling Pathway Diagram
Progesterone-mediated Oocyte Maturation Pathway antibodies	Progesterone-mediated Oocyte Maturation Pathway Diagram
Progesterone-mediated Oocyte Maturation Pathway antibodies	Progesterone-mediated Oocyte Maturation Pathway Diagram
P53 Signaling Pathway antibodies	P53 Signaling Pathway Diagram

Diseases associated with CCNB3 sirna

Disease Name	Pubmed Publications
Osteosarcoma Antibodies	>8 publications with CCNB3 and Osteosarcoma
Lung Diseases Antibodies	>2 publications with CCNB3 and Lung Diseases
Atrophy Antibodies	>1 publications with CCNB3 and Atrophy

Organs/Tissues associated with CCNB3 sirna

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>10 publications with CCNB3 and Bone
Testis Antibodies	>8 publications with CCNB3 and Testis
Brain Antibodies	>1 publications with CCNB3 and Brain