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CD2AP siRNA – CD2-Associated Protein (Human)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8229274

MBS8229274 is an siRNA targeting CD2AP. Also known as CD2AP siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit CD2AP expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD2AP, siRNA
Full Product Name
CD2AP siRNA (Human)
Product Gene Name
CD2AP sirna
Product Synonym Names
CD2-associated protein; Adapter protein CMS; Cas ligand with multiple SH3 domains
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
CD2AP siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
71,451 Da
OMIM
604241
3D Structure
ModBase 3D Structure for Q9Y5K6
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CD2AP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for CD2AP sirna
RNA Interference (RNAi)

Other Notes

Small volumes of CD2AP sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD2AP. It may not necessarily be applicable to this product.

NCBI GI #
11321634
NCBI GeneID
23607
NCBI Accession #
NP_036252.1
UniProt Primary Accession #
Q9Y5K6
UniProt Secondary Accession #
Q5VYA3; Q9UG97; A6NL34
UniProt Related Accession #
Q9Y5K6
GenBank Nucleotide #
NM_012120.2
NCBI Official Full Name
CD2-associated protein
NCBI Official Synonym Full Names
CD2-associated protein
NCBI Official Symbol
CD2AP
NCBI Official Synonym Symbols
CMS
UniProt Protein Name
CD2-associated protein
UniProt Synonym Protein Names
Adapter protein CMS; Cas ligand with multiple SH3 domains
Protein Family
CD2-associated protein
UniProt Gene Name
CD2AP
UniProt Entry Name
CD2AP_HUMAN
NCBI Protein Information
CD2-associated protein

NCBI Summary for CD2AP

This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease. [provided by RefSeq, Jul 2008]
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UniProt Comments for CD2AP

CD2AP: an adapter protein between membrane proteins and the actin cytoskeleton. May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Required for cytokinesis. Links CD2 engagement to the WASp-evoked actin polymerization required for synapse formation and T cell activation. Attaches to membrane proteins via its three SH3 domains and attaches to and rearranges the cytoskeleton via its C-terminus. CD2AP?s interactions with the cytoskeleton result in receptor clustering and endocysosis as well as targeting proteins to degradative vesicles in the kidney. Mice carrying a heterozygous knockout of CD2AP show defects in kidney filtration, and mutations in the human gene are associated with focal segmental glomerulosclerosis (FSGS3).

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: ruffle; filamentous actin; cytoplasm; nucleolus; plasma membrane; intercellular junction; actin cytoskeleton

Molecular Function: protein binding; structural constituent of cytoskeleton; SH3 domain binding

Biological Process: mitosis; cell division; protein complex assembly; signal transduction; substrate-bound cell migration, cell extension; negative regulation of transforming growth factor-beta1 production

Disease: Focal Segmental Glomerulosclerosis 3, Susceptibility To
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Research Articles on CD2AP

1. CD2AP gene variants may contribute to susceptibility to end-stage renal disease in patients with type 1 diabetes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CD2AP sirna

 Reference Product  PubMed Publications
 CD2 sirna  >167 publications with CD2AP and CD2
 NPHS2 sirna  >63 publications with CD2AP and NPHS2
 NPHS1 sirna  >25 publications with CD2AP and NPHS1
 CBL sirna  >19 publications with CD2AP and CBL
 FAM125A sirna  >4 publications with CD2AP and FAM125A
 KIRREL sirna  >3 publications with CD2AP and KIRREL
 FYN sirna  >2 publications with CD2AP and FYN
 BCAR1 sirna  >1 publications with CD2AP and BCAR1

Pathways associated with CD2AP sirna

 Products by Pathway  Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 Nephrin Interactions Pathway antibodies  Nephrin Interactions Pathway Diagram
 Nephrin/Neph1 Signaling In The Kidney Podocyte Pathway antibodies  Nephrin/Neph1 Signaling In The Kidney Podocyte Pathway Diagram
 VEGFR1 Specific Signals Pathway antibodies  VEGFR1 Specific Signals Pathway Diagram

Diseases associated with CD2AP sirna

 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >113 publications with CD2AP and Kidney Diseases
 Proteinuria Antibodies  >92 publications with CD2AP and Proteinuria
 Glomerulosclerosis, Focal Segmental Antibodies  >47 publications with CD2AP and Glomerulosclerosis, Focal Segmental
 Alzheimer Disease Antibodies  >23 publications with CD2AP and Alzheimer Disease
 Renal Insufficiency Antibodies  >16 publications with CD2AP and Renal Insufficiency
 Inflammation Antibodies  >9 publications with CD2AP and Inflammation
 Fibrosis Antibodies  >7 publications with CD2AP and Fibrosis
 Necrosis Antibodies  >5 publications with CD2AP and Necrosis
 Hypertension Antibodies  >4 publications with CD2AP and Hypertension
 Acute Kidney Injury Antibodies  >3 publications with CD2AP and Acute Kidney Injury

Organs/Tissues associated with CD2AP sirna

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >176 publications with CD2AP and Kidney
 Blood Antibodies  >40 publications with CD2AP and Blood
 Brain Antibodies  >11 publications with CD2AP and Brain
 Bone Antibodies  >11 publications with CD2AP and Bone
 Skin Antibodies  >7 publications with CD2AP and Skin
 Embryonic Tissue Antibodies  >6 publications with CD2AP and Embryonic Tissue
 Bone Marrow Antibodies  >6 publications with CD2AP and Bone Marrow
 Muscle Antibodies  >5 publications with CD2AP and Muscle
 Lymph Node Antibodies  >4 publications with CD2AP and Lymph Node
 Eye Antibodies  >4 publications with CD2AP and Eye
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