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CDC20 siRNA – Cell Division Cycle Protein 20 Homolog (Human)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8240783

MBS8240783 is an siRNA targeting CDC20. Also known as CDC20 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit CDC20 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CDC20, siRNA
Full Product Name
CDC20 siRNA (Human)
Product Gene Name
CDC20 sirna
Product Synonym Names
Cell division cycle protein 20 homolog; p55CDC
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
CDC20 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
54,723 Da
OMIM
603618
3D Structure
ModBase 3D Structure for Q12834
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CDC20 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for CDC20 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of CDC20 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CDC20. It may not necessarily be applicable to this product.

NCBI GI #
118402582
NCBI GeneID
991
NCBI Accession #
NP_001246.2
UniProt Primary Accession #
Q12834
UniProt Secondary Accession #
Q5JUY4; Q9BW56; Q9UQI9; B2R6Z6; D3DPJ1
UniProt Related Accession #
Q12834
GenBank Nucleotide #
NM_001255.2
NCBI Official Full Name
cell division cycle protein 20 homolog
NCBI Official Synonym Full Names
cell division cycle 20
NCBI Official Symbol
CDC20
NCBI Official Synonym Symbols
CDC20A; p55CDC; bA276H19.3
UniProt Protein Name
Cell division cycle protein 20 homolog
UniProt Synonym Protein Names
p55CDC
Protein Family
Anaphase-promoting complex
UniProt Gene Name
CDC20
UniProt Entry Name
CDC20_HUMAN
NCBI Protein Information
cell division cycle protein 20 homolog

NCBI Summary for CDC20

CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. [provided by RefSeq, Jul 2008]

UniProt Comments for CDC20

CDC20: Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. Found in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with SPATC1. Interacts with NEUROD2. Interacts with MAD2L1 and BUB1B. The phosphorylated form interacts with APC/C. Interacts with NINL. May interact with MAD2L2. Interacts with CDK5RAP2. Interacts with isoform 1 of NEK2. Belongs to the WD repeat CDC20/Fizzy family.

Protein type: Cell cycle regulation; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: nucleoplasm; spindle pole; centrosome; anaphase-promoting complex; perinuclear region of cytoplasm; cytoplasm; spindle; nucleus; cytosol

Molecular Function: protein C-terminus binding; protein binding; enzyme binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of meiosis; regulation of dendrite development; positive regulation of synaptic plasticity; protein ubiquitination; cell cycle; anaphase-promoting complex activation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; positive regulation of cell proliferation; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle
Show more

Research Articles on CDC20

1. a Cdc20 binding site in BubR1 facilitates both spindle assembly checkpoint signalling and silencing

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CDC20 sirna

 Reference Product  PubMed Publications
 CCNB1 sirna  >112 publications with CDC20 and CCNB1
 MAD2L1 sirna  >107 publications with CDC20 and MAD2L1
 BUB3 sirna  >56 publications with CDC20 and BUB3
 CDC27 sirna  >31 publications with CDC20 and CDC27
 CDC23 sirna  >18 publications with CDC20 and CDC23
 BUB1B sirna  >18 publications with CDC20 and BUB1B
 CDC16 sirna  >13 publications with CDC20 and CDC16
 ANAPC2 sirna  >8 publications with CDC20 and ANAPC2
 ANAPC4 sirna  >5 publications with CDC20 and ANAPC4
 PTTG1 sirna  >4 publications with CDC20 and PTTG1

Pathways associated with CDC20 sirna

 Products by Pathway  Pathway Diagram
 APC-Cdc20 Mediated Degradation Of Nek2A Pathway antibodies  APC-Cdc20 Mediated Degradation Of Nek2A Pathway Diagram
 APC/C Complex Pathway antibodies  APC/C Complex Pathway Diagram
 APC/C Complex Pathway antibodies  APC/C Complex Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Securin Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Securin Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway antibodies  APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway Diagram
 APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway antibodies  APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway Diagram
 Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram

Diseases associated with CDC20 sirna

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >47 publications with CDC20 and Carcinoma
 Breast Neoplasms Antibodies  >20 publications with CDC20 and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >18 publications with CDC20 and Cell Transformation, Neoplastic
 Adenocarcinoma Antibodies  >16 publications with CDC20 and Adenocarcinoma
 Lung Neoplasms Antibodies  >14 publications with CDC20 and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >12 publications with CDC20 and Neoplasms, Experimental
 Lung Diseases Antibodies  >11 publications with CDC20 and Lung Diseases
 Carcinoma, Squamous Cell Antibodies  >9 publications with CDC20 and Carcinoma, Squamous Cell
 Disease Models, Animal Antibodies  >9 publications with CDC20 and Disease Models, Animal
 Prostatic Neoplasms Antibodies  >8 publications with CDC20 and Prostatic Neoplasms

Organs/Tissues associated with CDC20 sirna

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >30 publications with CDC20 and Brain
 Lung Antibodies  >24 publications with CDC20 and Lung
 Liver Antibodies  >23 publications with CDC20 and Liver
 Blood Antibodies  >19 publications with CDC20 and Blood
 Prostate Antibodies  >12 publications with CDC20 and Prostate
 Bone Antibodies  >11 publications with CDC20 and Bone
 Bone Marrow Antibodies  >7 publications with CDC20 and Bone Marrow
 Nerve Antibodies  >6 publications with CDC20 and Nerve
 Muscle Antibodies  >6 publications with CDC20 and Muscle
 Embryonic Tissue Antibodies  >5 publications with CDC20 and Embryonic Tissue
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