CDC42 siRNA – Cell Division Control Protein 42 Homolog Isoform 1 (Human)

Name: CDC42 siRNA; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8237145
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8237145

MBS8237145 is an siRNA targeting CDC42. Also known as CDC42 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit CDC42 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CDC42, siRNA

Full Product Name

CDC42 siRNA (Human)

Product Gene Name

CDC42 sirna

Product Synonym Names

Cell division control protein 42 homolog; G25K GTP-binding protein

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

CDC42 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

21,311 Da

OMIM

116952

3D Structure

ModBase 3D Structure for P60953

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CDC42 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for CDC42 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of CDC42 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CDC42. It may not necessarily be applicable to this product.

NCBI GI #

89903012

NCBI GeneID

998

NCBI Accession #

NP_001034891.1

UniProt Primary Accession #

P60953

UniProt Secondary Accession #

P21181; P25763; Q7L8R5; Q9UDI2

UniProt Related Accession #

P60953

GenBank Nucleotide #

NM_001039802.1

NCBI Official Full Name

cell division control protein 42 homolog isoform 1

NCBI Official Synonym Full Names

cell division cycle 42

NCBI Official Symbol

CDC42

NCBI Official Synonym Symbols

G25K; CDC42Hs

UniProt Protein Name

Cell division control protein 42 homolog

UniProt Synonym Protein Names

G25K GTP-binding protein

Protein Family

Cdc42

UniProt Gene Name

CDC42

UniProt Entry Name

CDC42_HUMAN

NCBI Protein Information

cell division control protein 42 homolog

NCBI Summary for CDC42

The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013]

UniProt Comments for CDC42

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration.

Research Articles on CDC42

1. Cdc42 overexpression significantly improved the ability of cervical cancer cells to migrate possibly due to improved pseudopodia formation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CDC42 sirna

Reference Product	PubMed Publications
PAK1 sirna	>313 publications with CDC42 and PAK1
IQGAP1 sirna	>118 publications with CDC42 and IQGAP1
PAK2 sirna	>89 publications with CDC42 and PAK2
WASL sirna	>82 publications with CDC42 and WASL
ARHGDIA sirna	>29 publications with CDC42 and ARHGDIA
BAIAP2 sirna	>25 publications with CDC42 and BAIAP2
PARD6A sirna	>14 publications with CDC42 and PARD6A
ITSN1 sirna	>8 publications with CDC42 and ITSN1
ARHGAP1 sirna	>7 publications with CDC42 and ARHGAP1
PARD6B sirna	>1 publications with CDC42 and PARD6B

Pathways associated with CDC42 sirna

Products by Pathway	Pathway Diagram
AGE/RAGE Pathway antibodies	AGE/RAGE Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Adherens Junction Pathway antibodies	Adherens Junction Pathway Diagram
Adherens Junction Pathway antibodies	Adherens Junction Pathway Diagram
Androgen Receptor Signaling Pathway antibodies	Androgen Receptor Signaling Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
BDNF Signaling Pathway antibodies	BDNF Signaling Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram

Diseases associated with CDC42 sirna

Disease Name	Pubmed Publications
Carcinoma Antibodies	>240 publications with CDC42 and Carcinoma
Nervous System Diseases Antibodies	>145 publications with CDC42 and Nervous System Diseases
Inflammation Antibodies	>130 publications with CDC42 and Inflammation
Breast Neoplasms Antibodies	>129 publications with CDC42 and Breast Neoplasms
Disease Models, Animal Antibodies	>104 publications with CDC42 and Disease Models, Animal
Necrosis Antibodies	>99 publications with CDC42 and Necrosis
Brain Diseases Antibodies	>83 publications with CDC42 and Brain Diseases
Cardiovascular Diseases Antibodies	>83 publications with CDC42 and Cardiovascular Diseases
Lung Diseases Antibodies	>78 publications with CDC42 and Lung Diseases
Neoplasms, Experimental Antibodies	>59 publications with CDC42 and Neoplasms, Experimental

Organs/Tissues associated with CDC42 sirna

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>614 publications with CDC42 and Blood
Brain Antibodies	>494 publications with CDC42 and Brain
Nerve Antibodies	>332 publications with CDC42 and Nerve
Vascular Antibodies	>321 publications with CDC42 and Vascular
Muscle Antibodies	>240 publications with CDC42 and Muscle
Kidney Antibodies	>225 publications with CDC42 and Kidney
Lung Antibodies	>179 publications with CDC42 and Lung
Embryonic Tissue Antibodies	>165 publications with CDC42 and Embryonic Tissue
Bone Antibodies	>155 publications with CDC42 and Bone
Liver Antibodies	>131 publications with CDC42 and Liver