• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CDT1 siRNA – DNA Replication Factor Cdt1 (Human)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8215600

MBS8215600 is an siRNA targeting CDT1. Also known as CDT1 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit CDT1 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CDT1, siRNA
Full Product Name
CDT1 siRNA (Human)
Product Gene Name
CDT1 sirna
Product Synonym Names
DNA replication factor Cdt1; Double parked homolog; DUP
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
CDT1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
60,390 Da
OMIM
605525
3D Structure
ModBase 3D Structure for Q9H211
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CDT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for CDT1 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of CDT1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CDT1. It may not necessarily be applicable to this product.

NCBI GI #
188497689
NCBI GeneID
81620
NCBI Accession #
NP_112190.2
UniProt Primary Accession #
Q9H211
UniProt Secondary Accession #
Q86XX9; Q96CJ5; Q96GK5; Q96H67; Q96HE6; Q9BWM0
UniProt Related Accession #
Q9H211
GenBank Nucleotide #
NM_030928.3
NCBI Official Full Name
DNA replication factor Cdt1
NCBI Official Synonym Full Names
chromatin licensing and DNA replication factor 1
NCBI Official Symbol
CDT1
NCBI Official Synonym Symbols
DUP; RIS2
UniProt Protein Name
DNA replication factor Cdt1
UniProt Synonym Protein Names
Double parked homolog; DUP
Protein Family
Cell division cycle protein
UniProt Gene Name
CDT1
UniProt Synonym Gene Names
DUP
UniProt Entry Name
CDT1_HUMAN
NCBI Protein Information
DNA replication factor Cdt1

NCBI Summary for CDT1

The protein encoded by this gene is involved in the formation of the pre-replication complex that is necessary for DNA replication. The encoded protein can bind geminin, which prevents replication and may function to prevent this protein from initiating replication at inappropriate origins. Phosphorylation of this protein by cyclin A-dependent kinases results in degradation of the protein. [provided by RefSeq, Mar 2011]
Show more

UniProt Comments for CDT1

CDT1: an essential licensing protein for DNA replication. Cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Elevated in all human tumor cell lines analyzed. May function as an oncogene in mammals. Interaction with GMNN inhibits both binding of the MCM complex to origins of replication and DNA-binding activity. Present during G1 and early S phase of the cell cycle. Degraded during the late S, G2, and M phases.

Protein type: Oncoprotein; Motility/polarity/chemotaxis; DNA replication; DNA-binding

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: nucleoplasm; cytosol; nucleus

Molecular Function: protein binding; DNA binding

Biological Process: regulation of DNA replication initiation; G1/S-specific transcription in mitotic cell cycle; mitotic cell cycle; DNA replication; DNA replication checkpoint; regulation of DNA replication during S phase; G1/S transition of mitotic cell cycle

Disease: Meier-gorlin Syndrome 4
Show more

Research Articles on CDT1

1. ATM silencing induced partial reduction in levels of Skp2, a component of SCF(Skp2) ubiquitin ligase that controls Cdt1 degradation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CDT1 sirna

 Reference Product  PubMed Publications
 CDC6 sirna  >128 publications with CDT1 and CDC6
 GMNN sirna  >103 publications with CDT1 and GMNN
 PCNA sirna  >56 publications with CDT1 and PCNA
 MCM7 sirna  >29 publications with CDT1 and MCM7
 CDK2 sirna  >26 publications with CDT1 and CDK2
 ORC1 sirna  >25 publications with CDT1 and ORC1
 SKP2 sirna  >22 publications with CDT1 and SKP2
 MCM6 sirna  >20 publications with CDT1 and MCM6
 UBC sirna  >1 publications with CDT1 and UBC

Pathways associated with CDT1 sirna

 Products by Pathway  Pathway Diagram
 Activation Of The Pre-replicative Complex Pathway antibodies  Activation Of The Pre-replicative Complex Pathway Diagram
 Assembly Of The Pre-replicative Complex Pathway antibodies  Assembly Of The Pre-replicative Complex Pathway Diagram
 Association Of Licensing Factors With The Pre-replicative Complex Pathway antibodies  Association Of Licensing Factors With The Pre-replicative Complex Pathway Diagram
 CDT1 Association With The CDC6:ORC:origin Complex Pathway antibodies  CDT1 Association With The CDC6:ORC:origin Complex Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pre-Initiation Pathway antibodies  DNA Replication Pre-Initiation Pathway Diagram
 E2F Mediated Regulation Of DNA Replication Pathway antibodies  E2F Mediated Regulation Of DNA Replication Pathway Diagram

Diseases associated with CDT1 sirna

 Disease Name  Pubmed Publications
 Cell Transformation, Neoplastic Antibodies  >9 publications with CDT1 and Cell Transformation, Neoplastic
 Meier-Gorlin syndrome Antibodies  >8 publications with CDT1 and Meier-Gorlin syndrome
 Breast Neoplasms Antibodies  >6 publications with CDT1 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >4 publications with CDT1 and Neoplasms, Experimental
 Precancerous Conditions Antibodies  >3 publications with CDT1 and Precancerous Conditions
 Lung Neoplasms Antibodies  >3 publications with CDT1 and Lung Neoplasms
 Hyperplasia Antibodies  >3 publications with CDT1 and Hyperplasia
 Disease Models, Animal Antibodies  >2 publications with CDT1 and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >2 publications with CDT1 and Cardiovascular Diseases
 Neoplasm Metastasis Antibodies  >1 publications with CDT1 and Neoplasm Metastasis

Organs/Tissues associated with CDT1 sirna

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >11 publications with CDT1 and Bone
 Brain Antibodies  >10 publications with CDT1 and Brain
 Blood Antibodies  >10 publications with CDT1 and Blood
 Lung Antibodies  >9 publications with CDT1 and Lung
 Ovary Antibodies  >7 publications with CDT1 and Ovary
 Eye Antibodies  >6 publications with CDT1 and Eye
 Prostate Antibodies  >5 publications with CDT1 and Prostate
 Bone Marrow Antibodies  >5 publications with CDT1 and Bone Marrow
 Embryonic Tissue Antibodies  >4 publications with CDT1 and Embryonic Tissue
 Kidney Antibodies  >3 publications with CDT1 and Kidney
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000