CTSE siRNA – Cathepsin E Isoform a Preproprotein (Human)

Name: CTSE siRNA; Cathepsin E Isoform a Preproprotein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8223371
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8223371

MBS8223371 is an siRNA targeting CTSE. Also known as CTSE siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit CTSE expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CTSE, siRNA

Full Product Name

CTSE siRNA (Human)

Product Gene Name

CTSE sirna

Product Synonym Names

Cathepsin E

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

CTSE siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

39,553 Da

OMIM

116890

3D Structure

ModBase 3D Structure for P14091

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CTSE gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for CTSE sirna

RNA Interference (RNAi)

Other Notes

Small volumes of CTSE sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CTSE. It may not necessarily be applicable to this product.

NCBI GI #

4503145

NCBI GeneID

1510

NCBI Accession #

NP_001901.1

UniProt Primary Accession #

P14091

UniProt Secondary Accession #

Q5TZ01; Q5TZ02; Q9NY58; Q9UCE3; Q9UCE4

UniProt Related Accession #

P14091

GenBank Nucleotide #

NM_001910.3

NCBI Official Full Name

cathepsin E isoform a preproprotein

NCBI Official Synonym Full Names

cathepsin E

NCBI Official Symbol

CTSE

NCBI Official Synonym Symbols

CATE

UniProt Protein Name

Cathepsin E

Protein Family

Cathepsin

UniProt Gene Name

CTSE

UniProt Entry Name

CATE_HUMAN

NCBI Protein Information

cathepsin E

NCBI Summary for CTSE

The protein encoded by this gene is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease, which is a member of the peptidase A1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen. Transcript variants utilizing alternative polyadenylation signals and two transcript variants encoding different isoforms exist for this gene. [provided by RefSeq, Aug 2015]

UniProt Comments for CTSE

CTSE: May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain. Belongs to the peptidase A1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.23.34

Chromosomal Location of Human Ortholog: 1q31

Cellular Component: endosome

Molecular Function: protein homodimerization activity; aspartic-type endopeptidase activity

Biological Process: protein autoprocessing; digestion; antigen processing and presentation of exogenous peptide antigen via MHC class II; proteolysis

Research Articles on CTSE

1. Decreased activity of cathepsin E produced by decidual macrophages might be responsible for the induction of miscarriages in some recurrent miscarriage patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CTSE sirna

Reference Product	PubMed Publications
PCSK1 sirna	>1 publications with CTSE and PCSK1

Pathways associated with CTSE sirna

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Lysosome Pathway antibodies	Lysosome Pathway Diagram
Lysosome Pathway antibodies	Lysosome Pathway Diagram
MHC Class II Antigen Presentation Pathway antibodies	MHC Class II Antigen Presentation Pathway Diagram

Diseases associated with CTSE sirna

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>4 publications with CTSE and Disease Models, Animal
Liver Diseases Antibodies	>3 publications with CTSE and Liver Diseases
Inflammation Antibodies	>3 publications with CTSE and Inflammation
Urinary Bladder Neoplasms Antibodies	>2 publications with CTSE and Urinary Bladder Neoplasms
Hypertrophy Antibodies	>2 publications with CTSE and Hypertrophy
Fibrosis Antibodies	>2 publications with CTSE and Fibrosis
Neoplasms, Experimental Antibodies	>2 publications with CTSE and Neoplasms, Experimental
Breast Neoplasms Antibodies	>2 publications with CTSE and Breast Neoplasms
Hyperplasia Antibodies	>2 publications with CTSE and Hyperplasia
Necrosis Antibodies	>2 publications with CTSE and Necrosis

Organs/Tissues associated with CTSE sirna

Organ/Tissue Name	Pubmed Publications
Stomach Antibodies	>8 publications with CTSE and Stomach
Liver Antibodies	>3 publications with CTSE and Liver
Lung Antibodies	>3 publications with CTSE and Lung
Pancreas Antibodies	>3 publications with CTSE and Pancreas
Bone Marrow Antibodies	>2 publications with CTSE and Bone Marrow
Bladder Antibodies	>2 publications with CTSE and Bladder
Muscle Antibodies	>1 publications with CTSE and Muscle
Brain Antibodies	>1 publications with CTSE and Brain
Intestine Antibodies	>1 publications with CTSE and Intestine