DCD siRNA – Dermcidin Isoform 2 Preproprotein (Human)

Name: DCD siRNA; Dermcidin Isoform 2 Preproprotein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8212128
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8212128

MBS8212128 is an siRNA targeting DCD. Also known as DCD siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit DCD expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DCD, siRNA

Full Product Name

DCD siRNA (Human)

Product Gene Name

DCD sirna

Product Synonym Names

AIDD; DSEP; Dermcidin; Preproteolysin

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

DCD siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

8,295 Da

OMIM

606634

3D Structure

ModBase 3D Structure for P81605

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DCD gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for DCD sirna

RNA Interference (RNAi)

Other Notes

Small volumes of DCD sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DCD. It may not necessarily be applicable to this product.

NCBI GI #

665505971

NCBI GeneID

117159

NCBI Accession #

NP_001287783.1

UniProt Primary Accession #

P81605

UniProt Secondary Accession #

P58461; Q53YJ2; A5JHP2; A5JHP3

UniProt Related Accession #

P81605

GenBank Nucleotide #

NM_001300854.1

NCBI Official Full Name

dermcidin isoform 2 preproprotein

NCBI Official Synonym Full Names

dermcidin

NCBI Official Symbol

DCD

NCBI Official Synonym Symbols

PIF; AIDD; DSEP; HCAP; DCD-1

UniProt Protein Name

Dermcidin

UniProt Synonym Protein Names

Preproteolysin

Protein Family

Bifunctional D-cysteine desulfhydrase/1-aminocyclopropane-1-carboxylate deaminase

UniProt Gene Name

DCD

UniProt Synonym Gene Names

AIDD; DSEP

UniProt Entry Name

DCD_HUMAN

NCBI Protein Information

dermcidin

NCBI Summary for DCD

This antimicrobial gene encodes a secreted protein that is subsequently processed into mature peptides of distinct biological activities. The C-terminal peptide is constitutively expressed in sweat and has antibacterial and antifungal activities. The N-terminal peptide, also known as diffusible survival evasion peptide, promotes neural cell survival under conditions of severe oxidative stress. A glycosylated form of the N-terminal peptide may be associated with cachexia (muscle wasting) in cancer patients. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]

UniProt Comments for DCD

DCD: DCD-1 displays antimicrobial activity thereby limiting skin infection by potential pathogens in the first few hours after bacterial colonization. Highly effective against E.coli, E.faecalis, S.aureus and C.albicans. Optimal pH and salt concentration resemble the conditions in sweat. Also exhibits proteolytic activity. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; EC 3.4.-.-

Chromosomal Location of Human Ortholog: 12q13.1

Cellular Component: extracellular region

Molecular Function: peptidase activity

Biological Process: killing of cells of another organism; defense response to bacterium; proteolysis; defense response to fungus

Research Articles on DCD

1. Data indicate that Y-P30/dermcidin expressed in placentas of first trimester pregnancies.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DCD sirna

Reference Product	PubMed Publications
RELA sirna	>3 publications with DCD and RELA
CAMP sirna	>3 publications with DCD and CAMP
UBC sirna	>3 publications with DCD and UBC

Diseases associated with DCD sirna

Disease Name	Pubmed Publications
Death Antibodies	>615 publications with DCD and Death
Cardiovascular Diseases Antibodies	>185 publications with DCD and Cardiovascular Diseases
Heart Diseases Antibodies	>123 publications with DCD and Heart Diseases
Kidney Diseases Antibodies	>54 publications with DCD and Kidney Diseases
Necrosis Antibodies	>48 publications with DCD and Necrosis
Inflammation Antibodies	>36 publications with DCD and Inflammation
Renal Insufficiency Antibodies	>29 publications with DCD and Renal Insufficiency
Memory Disorders Antibodies	>27 publications with DCD and Memory Disorders
Lung Injury Antibodies	>23 publications with DCD and Lung Injury
Fibrosis Antibodies	>19 publications with DCD and Fibrosis

Organs/Tissues associated with DCD sirna

Organ/Tissue Name	Pubmed Publications
Uncharacterized Tissue Antibodies	>1 publications with DCD and Uncharacterized Tissue