DGKH siRNA – Diacylglycerol Kinase Eta Isoform 1 (Human)

Name: DGKH siRNA; Diacylglycerol Kinase Eta Isoform 1; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8233820
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8233820

MBS8233820 is an siRNA targeting DGKH. Also known as DGKH siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit DGKH expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DGKH, siRNA

Full Product Name

DGKH siRNA (Human)

Product Gene Name

DGKH sirna

Product Synonym Names

Diacylglycerol kinase eta; DAG kinase eta; Diglyceride kinase eta; DGK-eta

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

DGKH siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

120,566 Da

OMIM

604071

3D Structure

ModBase 3D Structure for Q86XP1

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DGKH gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for DGKH sirna

RNA Interference (RNAi)

Other Notes

Small volumes of DGKH sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DGKH. It may not necessarily be applicable to this product.

NCBI GI #

325197213

NCBI GeneID

160851

NCBI Accession #

NP_001191433.1

UniProt Primary Accession #

Q86XP1

UniProt Secondary Accession #

Q5VZW0; Q6PI56; Q86XP2; Q8N3N0; Q8N7J9; A2A2W7; A6NFX7; B4DZ34

UniProt Related Accession #

Q86XP1

GenBank Nucleotide #

NM_001204504.2

NCBI Official Full Name

diacylglycerol kinase eta isoform 1

NCBI Official Synonym Full Names

diacylglycerol kinase, eta

NCBI Official Symbol

DGKH

NCBI Official Synonym Symbols

DGKeta

UniProt Protein Name

Diacylglycerol kinase eta

UniProt Synonym Protein Names

Diglyceride kinase eta; DGK-eta

UniProt Gene Name

DGKH

UniProt Synonym Gene Names

DAG kinase eta; DGK-eta

UniProt Entry Name

DGKH_HUMAN

NCBI Protein Information

diacylglycerol kinase eta

NCBI Summary for DGKH

This gene encodes a member of the diacylglycerol kinase (DGK) enzyme family. Members of this family are involved in regulating intracellular concentrations of diacylglycerol and phosphatidic acid. Variation in this gene has been associated with bipolar disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Jul 2014]

UniProt Comments for DGKH

DGKH: Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization. Belongs to the eukaryotic diacylglycerol kinase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.1.107; Lipid Metabolism - glycerophospholipid; Motility/polarity/chemotaxis; Lipid Metabolism - glycerolipid; Kinase, other

Chromosomal Location of Human Ortholog: 13q14.11

Cellular Component: nuclear membrane; cytoplasm; plasma membrane; nucleus; endosome

Molecular Function: metal ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: platelet activation; protein kinase C activation; blood coagulation; phosphorylation; protein oligomerization

Research Articles on DGKH

1. DGKK variants are associated with hypospadias

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with DGKH sirna

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Choline Metabolism In Cancer Pathway antibodies	Choline Metabolism In Cancer Pathway Diagram
Choline Metabolism In Cancer Pathway antibodies	Choline Metabolism In Cancer Pathway Diagram
Effects Of PIP2 Hydrolysis Pathway antibodies	Effects Of PIP2 Hydrolysis Pathway Diagram
G Alpha (q) Signalling Events Pathway antibodies	G Alpha (q) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies	Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
Glycerolipid Metabolism Pathway antibodies	Glycerolipid Metabolism Pathway Diagram
Glycerolipid Metabolism Pathway antibodies	Glycerolipid Metabolism Pathway Diagram
Glycerophospholipid Metabolism Pathway antibodies	Glycerophospholipid Metabolism Pathway Diagram
Glycerophospholipid Metabolism Pathway antibodies	Glycerophospholipid Metabolism Pathway Diagram

Diseases associated with DGKH sirna

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>2 publications with DGKH and Disease Models, Animal
Kidney Diseases Antibodies	>2 publications with DGKH and Kidney Diseases
Precancerous Conditions Antibodies	>1 publications with DGKH and Precancerous Conditions
Anxiety Disorders Antibodies	>1 publications with DGKH and Anxiety Disorders
Hyperplasia Antibodies	>1 publications with DGKH and Hyperplasia
Memory Disorders Antibodies	>1 publications with DGKH and Memory Disorders

Organs/Tissues associated with DGKH sirna

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>5 publications with DGKH and Brain
Kidney Antibodies	>2 publications with DGKH and Kidney
Eye Antibodies	>1 publications with DGKH and Eye
Heart Antibodies	>1 publications with DGKH and Heart
Mouth Antibodies	>1 publications with DGKH and Mouth
Adrenal Gland Antibodies	>1 publications with DGKH and Adrenal Gland
Muscle Antibodies	>1 publications with DGKH and Muscle