DPH6 siRNA – Diphthine--Ammonia Ligase Isoform 2 (Human)

Name: DPH6 siRNA; Diphthine--Ammonia Ligase Isoform 2; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8206307
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8206307

MBS8206307 is an siRNA targeting DPH6. Also known as DPH6 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit DPH6 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DPH6, siRNA

Full Product Name

DPH6 siRNA (Human)

Product Gene Name

DPH6 sirna

Product Synonym Names

ATPBD4; Diphthine--ammonia ligase; ATP-binding domain-containing protein 4; Diphthamide synthase; Diphthamide synthetase; Protein DPH6 homolog

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

DPH6 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

18,657 Da

3D Structure

ModBase 3D Structure for Q7L8W6

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DPH6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for DPH6 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of DPH6 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DPH6. It may not necessarily be applicable to this product.

NCBI GI #

213972615

NCBI GeneID

89978

NCBI Accession #

NP_001135444.1

UniProt Primary Accession #

Q7L8W6

UniProt Secondary Accession #

Q96HJ6; B3KWG1

UniProt Related Accession #

Q7L8W6

GenBank Nucleotide #

NM_001141972.1

NCBI Official Full Name

diphthine--ammonia ligase isoform 2

NCBI Official Synonym Full Names

diphthamine biosynthesis 6

NCBI Official Symbol

DPH6

NCBI Official Synonym Symbols

ATPBD4

UniProt Protein Name

Diphthine--ammonia ligase

UniProt Synonym Protein Names

ATP-binding domain-containing protein 4; Diphthamide synthase; Diphthamide synthetase; Protein DPH6 homolog

Protein Family

Diphthine--ammonia ligase

UniProt Gene Name

DPH6

UniProt Synonym Gene Names

ATPBD4

UniProt Entry Name

DPH6_HUMAN

NCBI Protein Information

diphthine--ammonia ligase

UniProt Comments for DPH6

DPH6: Amidase that catalyzes the last step of diphthamide biosynthesis using ammonium and ATP. Diphthamide biosynthesis consists in the conversion of an L-histidine residue in the translation elongation factor (EEF2) to diphthamide. Belongs to the Diphthine--ammonia ligase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.1.14

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: cytosol

Molecular Function: diphthine-ammonia ligase activity; ATP binding

Biological Process: cellular protein metabolic process; peptidyl-diphthamide biosynthetic process from peptidyl-histidine; post-translational protein modification

Research Articles on DPH6

1. The previously uncharacterized human gene ATPBD4 is the ortholog of yeast YLR143W, a diphthamide synthetase, and fully rescues the deletion of YLR143W in yeast.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DPH6 sirna

Reference Product	PubMed Publications
DPH5 sirna	>3 publications with DPH6 and DPH5
DPH1 sirna	>3 publications with DPH6 and DPH1
DPH3 sirna	>1 publications with DPH6 and DPH3
DPH2 sirna	>1 publications with DPH6 and DPH2
WDR85 sirna	>1 publications with DPH6 and WDR85

Pathways associated with DPH6 sirna

Products by Pathway	Pathway Diagram
Gamma Carboxylation, Hypusine Formation And Arylsulfatase Activation Pathway antibodies	Gamma Carboxylation, Hypusine Formation And Arylsulfatase Activation Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Post-translational Protein Modification Pathway antibodies	Post-translational Protein Modification Pathway Diagram
Synthesis Of Diphthamide-EEF2 Pathway antibodies	Synthesis Of Diphthamide-EEF2 Pathway Diagram
Diphthamide Biosynthesis Pathway antibodies	Diphthamide Biosynthesis Pathway Diagram

Diseases associated with DPH6 sirna

Disease Name	Pubmed Publications
Mental Disorders Antibodies	>1 publications with DPH6 and Mental Disorders
Nervous System Diseases Antibodies	>1 publications with DPH6 and Nervous System Diseases

Organs/Tissues associated with DPH6 sirna

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>1 publications with DPH6 and Brain