ECHS1 siRNA – Enoyl-Coa Hydratase, Mitochondrial (Human)

Name: ECHS1 siRNA; Enoyl-Coa Hydratase, Mitochondrial; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8230914
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8230914

MBS8230914 is an siRNA targeting ECHS1. Also known as ECHS1 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit ECHS1 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ECHS1, siRNA

Full Product Name

ECHS1 siRNA (Human)

Product Gene Name

ECHS1 sirna

Product Synonym Names

Enoyl-CoA hydratase mitochondrial; Enoyl-CoA hydratase 1; Short-chain enoyl-CoA hydratase; SCEH

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

ECHS1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

31,387 Da

OMIM

602292

3D Structure

ModBase 3D Structure for P30084

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ECHS1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for ECHS1 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of ECHS1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ECHS1. It may not necessarily be applicable to this product.

NCBI GI #

194097323

NCBI GeneID

1892

NCBI Accession #

NP_004083.3

UniProt Primary Accession #

P30084

UniProt Secondary Accession #

O00739; Q5VWY1; Q96H54

UniProt Related Accession #

P30084

GenBank Nucleotide #

NM_004092.3

NCBI Official Full Name

enoyl-CoA hydratase, mitochondrial

NCBI Official Synonym Full Names

enoyl CoA hydratase, short chain, 1, mitochondrial

NCBI Official Symbol

ECHS1

NCBI Official Synonym Symbols

SCEH; ECHS1D

UniProt Protein Name

Enoyl-CoA hydratase, mitochondrial

UniProt Synonym Protein Names

Enoyl-CoA hydratase 1; Short-chain enoyl-CoA hydratase; SCEH

Protein Family

Enoyl-CoA hydratase

UniProt Gene Name

ECHS1

UniProt Synonym Gene Names

SCEH

UniProt Entry Name

ECHM_HUMAN

NCBI Protein Information

enoyl-CoA hydratase, mitochondrial

NCBI Summary for ECHS1

The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. [provided by RefSeq, Jul 2008]

UniProt Comments for ECHS1

ECHS1: Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate. Belongs to the enoyl-CoA hydratase/isomerase family.

Protein type: Other Amino Acids Metabolism - beta-alanine; Amino Acid Metabolism - lysine degradation; Lipid Metabolism - fatty acid elongation in mitochondria; Carbohydrate Metabolism - propanoate; Lyase; Amino Acid Metabolism - valine, leucine and isoleucine degradation; EC 4.2.1.17; Carbohydrate Metabolism - butanoate; Secondary Metabolites Metabolism - limonene and pinene degradation; Lipid Metabolism - fatty acid; Amino Acid Metabolism - tryptophan; Mitochondrial

Chromosomal Location of Human Ortholog: 10q26.2-q26.3

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: protein binding; enoyl-CoA hydratase activity

Biological Process: fatty acid beta-oxidation; cellular lipid metabolic process

Disease: Mitochondrial Short-chain Enoyl-coa Hydratase 1 Deficiency

Research Articles on ECHS1

1. The study demonstrated that ECHS1 mutations result in ECHS1 deficiency and are another cause of Leigh disease in two siblings.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ECHS1 sirna

Reference Product	PubMed Publications
ACADS sirna	>4 publications with ECHS1 and ACADS
HADH sirna	>2 publications with ECHS1 and HADH
HADHA sirna	>1 publications with ECHS1 and HADHA
GCDH sirna	>1 publications with ECHS1 and GCDH
ACADM sirna	>1 publications with ECHS1 and ACADM

Pathways associated with ECHS1 sirna

Products by Pathway	Pathway Diagram
Beta Oxidation Of Butanoyl-CoA To Acetyl-CoA Pathway antibodies	Beta Oxidation Of Butanoyl-CoA To Acetyl-CoA Pathway Diagram
Beta Oxidation Of Decanoyl-CoA To Octanoyl-CoA-CoA Pathway antibodies	Beta Oxidation Of Decanoyl-CoA To Octanoyl-CoA-CoA Pathway Diagram
Beta Oxidation Of Hexanoyl-CoA To Butanoyl-CoA Pathway antibodies	Beta Oxidation Of Hexanoyl-CoA To Butanoyl-CoA Pathway Diagram
Beta Oxidation Of Lauroyl-CoA To Decanoyl-CoA-CoA Pathway antibodies	Beta Oxidation Of Lauroyl-CoA To Decanoyl-CoA-CoA Pathway Diagram
Beta Oxidation Of Octanoyl-CoA To Hexanoyl-CoA Pathway antibodies	Beta Oxidation Of Octanoyl-CoA To Hexanoyl-CoA Pathway Diagram
Biosynthesis Of Antibiotics Pathway antibodies	Biosynthesis Of Antibiotics Pathway Diagram
Butanoate Metabolism Pathway antibodies	Butanoate Metabolism Pathway Diagram
Butanoate Metabolism Pathway antibodies	Butanoate Metabolism Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram

Diseases associated with ECHS1 sirna

Disease Name	Pubmed Publications
Liver Diseases Antibodies	>12 publications with ECHS1 and Liver Diseases
Fatty Liver Antibodies	>12 publications with ECHS1 and Fatty Liver
Carcinoma, Hepatocellular Antibodies	>6 publications with ECHS1 and Carcinoma, Hepatocellular
Liver Neoplasms Antibodies	>5 publications with ECHS1 and Liver Neoplasms
Inflammation Antibodies	>4 publications with ECHS1 and Inflammation
Mitochondrial Diseases Antibodies	>3 publications with ECHS1 and Mitochondrial Diseases
Obesity Antibodies	>3 publications with ECHS1 and Obesity
Nervous System Diseases Antibodies	>2 publications with ECHS1 and Nervous System Diseases
Hyperplasia Antibodies	>2 publications with ECHS1 and Hyperplasia
Cardiovascular Diseases Antibodies	>1 publications with ECHS1 and Cardiovascular Diseases

Organs/Tissues associated with ECHS1 sirna

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>20 publications with ECHS1 and Liver
Brain Antibodies	>6 publications with ECHS1 and Brain
Muscle Antibodies	>4 publications with ECHS1 and Muscle
Blood Antibodies	>3 publications with ECHS1 and Blood
Testis Antibodies	>2 publications with ECHS1 and Testis
Thyroid Antibodies	>2 publications with ECHS1 and Thyroid
Prostate Antibodies	>2 publications with ECHS1 and Prostate
Nerve Antibodies	>2 publications with ECHS1 and Nerve
Lymph Node Antibodies	>2 publications with ECHS1 and Lymph Node
Lymph Antibodies	>2 publications with ECHS1 and Lymph