EFCAB6 siRNA – Ef-Hand Calcium-Binding Domain-Containing Protein 6 Isoform a (Human)

Name: EFCAB6 siRNA; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8206324
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8206324

MBS8206324 is an siRNA targeting EFCAB6. Also known as EFCAB6 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit EFCAB6 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

EFCAB6, siRNA

Full Product Name

EFCAB6 siRNA (Human)

Product Gene Name

EFCAB6 sirna

Product Synonym Names

DJBP; KIAA1672; EF-hand calcium-binding domain-containing protein 6; CAP-binding protein complex-interacting protein 1; DJ-1-binding protein; DJBP

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

EFCAB6 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

144,276 Da

3D Structure

ModBase 3D Structure for Q5THR3

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human EFCAB6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for EFCAB6 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of EFCAB6 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EFCAB6. It may not necessarily be applicable to this product.

NCBI GI #

38570107

NCBI GeneID

64800

NCBI Accession #

NP_073622.2

UniProt Primary Accession #

Q5THR3

UniProt Secondary Accession #

Q5U5T6; Q9BY88; Q9H5C4; Q9NSF5; A8K8P6; A8K8Y3; B0QYI4; B0QYI6

UniProt Related Accession #

Q5THR3

GenBank Nucleotide #

NM_022785.3

NCBI Official Full Name

EF-hand calcium-binding domain-containing protein 6 isoform a

NCBI Official Synonym Full Names

EF-hand calcium binding domain 6

NCBI Official Symbol

EFCAB6

NCBI Official Synonym Symbols

DJBP; HSCBCIP1; dJ185D5.1

UniProt Protein Name

EF-hand calcium-binding domain-containing protein 6

UniProt Synonym Protein Names

CAP-binding protein complex-interacting protein 1; DJ-1-binding protein; DJBP

Protein Family

EF-hand calcium-binding domain-containing protein

UniProt Gene Name

EFCAB6

UniProt Synonym Gene Names

DJBP; KIAA1672; DJBP

UniProt Entry Name

EFCB6_HUMAN

NCBI Protein Information

EF-hand calcium-binding domain-containing protein 6

NCBI Summary for EFCAB6

This gene encodes a protein which directly binds the oncogene DJ-1 and androgen receptor to form a ternary complex in cells. This binding protein recruits histone-deacetylase complexes in order to repress transcription activity of androgen receptor. This protein may also play a role in spermatogenesis and fertilization. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

UniProt Comments for EFCAB6

EFCAB6: Negatively regulates the androgen receptor by recruiting histone deacetylase complex, and protein DJ-1 antagonizes this inhibition by abrogation of this complex. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: nucleus

Molecular Function: calcium ion binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent

Research Articles on EFCAB6

1. Observational study of gene-disease association. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with EFCAB6 sirna

Reference Product	PubMed Publications
PARK7 sirna	>2 publications with EFCAB6 and PARK7
HDAC1 sirna	>1 publications with EFCAB6 and HDAC1

Pathways associated with EFCAB6 sirna

Products by Pathway	Pathway Diagram
Androgen Receptor Signaling Pathway antibodies	Androgen Receptor Signaling Pathway Diagram

Diseases associated with EFCAB6 sirna

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>1 publications with EFCAB6 and Nervous System Diseases

Organs/Tissues associated with EFCAB6 sirna

Organ/Tissue Name	Pubmed Publications
Testis Antibodies	>1 publications with EFCAB6 and Testis