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FHOD1 siRNA – FH1/FH2 Domain-Containing Protein 1 (Human)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8228845

MBS8228845 is an siRNA targeting FHOD1. Also known as FHOD1 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit FHOD1 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
FHOD1, siRNA
Full Product Name
FHOD1 siRNA (Human)
Product Gene Name
FHOD1 sirna
Product Synonym Names
FHOS; FHOS1; FH1/FH2 domain-containing protein 1; Formin homolog overexpressed in spleen 1; FHOS; Formin homology 2 domain-containing protein 1
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
FHOD1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
126,551 Da
OMIM
606881
3D Structure
ModBase 3D Structure for Q9Y613
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human FHOD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for FHOD1 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of FHOD1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FHOD1. It may not necessarily be applicable to this product.

NCBI GI #
118572599
NCBI GeneID
29109
NCBI Accession #
NP_037373.2
UniProt Primary Accession #
Q9Y613
UniProt Secondary Accession #
Q59F76; Q6Y1F2; Q76MS8; Q8N521
UniProt Related Accession #
Q9Y613
GenBank Nucleotide #
NM_013241.2
NCBI Official Full Name
FH1/FH2 domain-containing protein 1
NCBI Official Synonym Full Names
formin homology 2 domain containing 1
NCBI Official Symbol
FHOD1
NCBI Official Synonym Symbols
FHOS
UniProt Protein Name
FH1/FH2 domain-containing protein 1
UniProt Synonym Protein Names
Formin homolog overexpressed in spleen 1; FHOS; Formin homology 2 domain-containing protein 1
Protein Family
FH1/FH2 domain-containing protein
UniProt Gene Name
FHOD1
UniProt Synonym Gene Names
FHOS; FHOS1; FHOS
UniProt Entry Name
FHOD1_HUMAN
NCBI Protein Information
FH1/FH2 domain-containing protein 1

NCBI Summary for FHOD1

This gene encodes a protein which is a member of the formin/diaphanous family of proteins. The gene is ubiquitously expressed but is found in abundance in the spleen. The encoded protein has sequence homology to diaphanous and formin proteins within the Formin Homology (FH)1 and FH2 domains. It also contains a coiled-coil domain, a collagen-like domain, two nuclear localization signals, and several potential PKC and PKA phosphorylation sites. It is a predominantly cytoplasmic protein and is expressed in a variety of human cell lines. [provided by RefSeq, Jul 2008]
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UniProt Comments for FHOD1

FHOD1: a cytoskeletal protein required for the assembly of F-actin structures, such as stress fibers. Depends on the Rho-ROCK cascade for its activity. Contributes to the coordination of microtubules with actin fibers and plays a role in cell elongation. Self-associates via the FH2 domain. Binds to F-actin via its N-terminus. Binds to the cytoplasmic domain of CD21 via its C- terminus.

Protein type: Actin-binding

Chromosomal Location of Human Ortholog: 16q22

Cellular Component: membrane; cytoplasm; stress fiber; nucleus

Molecular Function: identical protein binding; protein binding; actin binding

Biological Process: positive regulation of stress fiber formation; positive regulation of transcription from RNA polymerase II promoter
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Research Articles on FHOD1

1. Report FODH1 expression in cardiomyocytes and increased expression in dilated cardiommyopathy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with FHOD1 sirna

 Reference Product  PubMed Publications
 FMNL2 sirna  >9 publications with FHOD1 and FMNL2
 FMNL1 sirna  >9 publications with FHOD1 and FMNL1
 RAC1 sirna  >9 publications with FHOD1 and RAC1
 FHDC1 sirna  >4 publications with FHOD1 and FHDC1
 ROCK1 sirna  >1 publications with FHOD1 and ROCK1
 RAC3 sirna  >1 publications with FHOD1 and RAC3

Diseases associated with FHOD1 sirna

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >1 publications with FHOD1 and Breast Neoplasms
 Osteosarcoma Antibodies  >1 publications with FHOD1 and Osteosarcoma
 Immune System Diseases Antibodies  >1 publications with FHOD1 and Immune System Diseases
 Skin Diseases Antibodies  >1 publications with FHOD1 and Skin Diseases
 Nervous System Diseases Antibodies  >1 publications with FHOD1 and Nervous System Diseases
 Fetal Death Antibodies  >1 publications with FHOD1 and Fetal Death
 Fibrosis Antibodies  >1 publications with FHOD1 and Fibrosis
 Neoplasm Metastasis Antibodies  >1 publications with FHOD1 and Neoplasm Metastasis

Organs/Tissues associated with FHOD1 sirna

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >7 publications with FHOD1 and Muscle
 Heart Antibodies  >6 publications with FHOD1 and Heart
 Spleen Antibodies  >4 publications with FHOD1 and Spleen
 Blood Antibodies  >4 publications with FHOD1 and Blood
 Kidney Antibodies  >3 publications with FHOD1 and Kidney
 Eye Antibodies  >3 publications with FHOD1 and Eye
 Embryonic Tissue Antibodies  >2 publications with FHOD1 and Embryonic Tissue
 Lung Antibodies  >2 publications with FHOD1 and Lung
 Prostate Antibodies  >2 publications with FHOD1 and Prostate
 Nerve Antibodies  >2 publications with FHOD1 and Nerve
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