GEMIN5 siRNA – Gem-Associated Protein 5 Isoform 2 (Human)

Name: GEMIN5 siRNA; Gem-Associated Protein 5 Isoform 2; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8221828
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8221828

MBS8221828 is an siRNA targeting GEMIN5. Also known as GEMIN5 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit GEMIN5 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GEMIN5, siRNA

Full Product Name

GEMIN5 siRNA (Human)

Product Gene Name

GEMIN5 sirna

Product Synonym Names

Gem-associated protein 5; Gemin5

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

GEMIN5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

168,589 Da

OMIM

607005

3D Structure

ModBase 3D Structure for Q8TEQ6

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GEMIN5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for GEMIN5 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of GEMIN5 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GEMIN5. It may not necessarily be applicable to this product.

NCBI GI #

356461016

NCBI GeneID

25929

NCBI Accession #

NP_001239085.1

UniProt Primary Accession #

Q8TEQ6

UniProt Secondary Accession #

Q14CV0; Q8WWV4; Q969W4; Q9H9K3; Q9UFI5

UniProt Related Accession #

Q8TEQ6

GenBank Nucleotide #

NM_001252156.1

NCBI Official Full Name

gem-associated protein 5 isoform 2

NCBI Official Synonym Full Names

gem (nuclear organelle) associated protein 5

NCBI Official Symbol

GEMIN5

NCBI Official Synonym Symbols

GEMIN-5

UniProt Protein Name

Gem-associated protein 5

Protein Family

Component of gems protein

UniProt Gene Name

GEMIN5

UniProt Synonym Gene Names

Gemin5

UniProt Entry Name

GEMI5_HUMAN

NCBI Protein Information

gem-associated protein 5

NCBI Summary for GEMIN5

This gene encodes a WD repeat protein that is a component of the survival of motor neurons (SMN) complex. The SMN complex plays a critical role in mRNA splicing through the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs), and may also mediate the assembly and transport of other classes of ribonucleoproteins. The encoded protein is the snRNA-binding component of the SMN complex. Dysregulation of this gene may play a role in alternative mRNA splicing and tumor cell motility. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

UniProt Comments for GEMIN5

GEMIN5: the snRNA-binding protein of the SMN complex. The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. Found both in the nucleoplasm and in nuclear bodies called gems (Gemini of Cajal bodies) that are often in proximity to Cajal (coiled) bodies. Also found in the cytoplasm.

Protein type: Nuclear receptor co-regulator; RNA splicing; RNA-binding; RNA processing

Chromosomal Location of Human Ortholog: 5q33.2

Cellular Component: nucleoplasm; nuclear body; membrane; cytoplasm; SMN complex; cytosol; nucleus

Molecular Function: protein binding; snRNA binding

Biological Process: nuclear mRNA splicing, via spliceosome; spliceosomal snRNP biogenesis; protein complex assembly; gene expression

Research Articles on GEMIN5

1. The C-terminal region of Gemin5 bears two non-canonical bipartite RNA-binding sites.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GEMIN5 sirna

Reference Product	PubMed Publications
GEMIN2 sirna	>13 publications with GEMIN5 and GEMIN2
GEMIN4 sirna	>8 publications with GEMIN5 and GEMIN4
GEMIN6 sirna	>7 publications with GEMIN5 and GEMIN6
GEMIN7 sirna	>5 publications with GEMIN5 and GEMIN7
DDX20 sirna	>5 publications with GEMIN5 and DDX20
SNRPD3 sirna	>1 publications with GEMIN5 and SNRPD3
SNRPD1 sirna	>1 publications with GEMIN5 and SNRPD1

Pathways associated with GEMIN5 sirna

Products by Pathway	Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Metabolism Of Non-coding RNA Pathway antibodies	Metabolism Of Non-coding RNA Pathway Diagram
RNA Transport Pathway antibodies	RNA Transport Pathway Diagram
RNA Transport Pathway antibodies	RNA Transport Pathway Diagram
Survival Motor Neuron (SMN) Complex Pathway antibodies	Survival Motor Neuron (SMN) Complex Pathway Diagram
Survival Motor Neuron (SMN) Complex Pathway antibodies	Survival Motor Neuron (SMN) Complex Pathway Diagram
SnRNP Assembly Pathway antibodies	SnRNP Assembly Pathway Diagram

Diseases associated with GEMIN5 sirna

Disease Name	Pubmed Publications
Atrophy Antibodies	>15 publications with GEMIN5 and Atrophy
Neoplasm Metastasis Antibodies	>2 publications with GEMIN5 and Neoplasm Metastasis
Breast Neoplasms Antibodies	>1 publications with GEMIN5 and Breast Neoplasms
Necrosis Antibodies	>1 publications with GEMIN5 and Necrosis

Organs/Tissues associated with GEMIN5 sirna

Organ/Tissue Name	Pubmed Publications
Mouth Antibodies	>4 publications with GEMIN5 and Mouth
Muscle Antibodies	>2 publications with GEMIN5 and Muscle
Kidney Antibodies	>1 publications with GEMIN5 and Kidney
Uncharacterized Tissue Antibodies	>1 publications with GEMIN5 and Uncharacterized Tissue
Brain Antibodies	>1 publications with GEMIN5 and Brain