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GLCE siRNA – D-Glucuronyl C5-Epimerase (Human)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8235102

MBS8235102 is an siRNA targeting GLCE. Also known as GLCE siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit GLCE expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GLCE, siRNA
Full Product Name
GLCE siRNA (Human)
Product Gene Name
GLCE sirna
Product Synonym Names
KIAA0836; D-glucuronyl C5-epimerase; Heparan sulfate C5-epimerase; Hsepi; Heparin/heparan sulfate:glucuronic acid C5-epimerase; Heparosan-N-sulfate-glucuronate 5-epimerase
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
GLCE siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
70,101 Da
OMIM
612134
3D Structure
ModBase 3D Structure for O94923
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GLCE gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for GLCE sirna
RNA Interference (RNAi)

Other Notes

Small volumes of GLCE sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GLCE. It may not necessarily be applicable to this product.

NCBI GI #
51317380
NCBI GeneID
26035
NCBI Accession #
NP_056369.1
UniProt Primary Accession #
O94923
UniProt Secondary Accession #
Q6GUQ2
UniProt Related Accession #
O94923
GenBank Nucleotide #
NM_015554.1
NCBI Official Full Name
D-glucuronyl C5-epimerase
NCBI Official Synonym Full Names
glucuronic acid epimerase
NCBI Official Symbol
GLCE
NCBI Official Synonym Symbols
HSEPI
UniProt Protein Name
D-glucuronyl C5-epimerase
UniProt Synonym Protein Names
Heparan sulfate C5-epimerase; Hsepi; Heparin/heparan sulfate:glucuronic acid C5-epimerase; Heparosan-N-sulfate-glucuronate 5-epimerase
Protein Family
D-glucuronyl C5-epimerase
UniProt Gene Name
GLCE
UniProt Synonym Gene Names
; Hsepi
UniProt Entry Name
GLCE_HUMAN
NCBI Protein Information
D-glucuronyl C5-epimerase

NCBI Summary for GLCE

Heparan sulfate (HS) is a negatively charged cell surface polysaccharide required for the biologic activities of circulating extracellular ligands. GLCE is responsible for epimerization of D-glucuronic acid (GlcA) to L-iduronic acid (IdoA) of HS, which endows the nascent polysaccharide chain with the ability to bind growth factors and cytokines (Ghiselli and Agrawal, 2005 [PubMed 15853773]).[supplied by OMIM, Jun 2008]
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UniProt Comments for GLCE

GLCE: Converts D-glucuronic acid residues adjacent to N- sulfate sugar residues to L-iduronic acid residues, both in maturing heparan sulfate (HS) and heparin chains. This is important for further modifications that determine the specificity of interactions between these glycosaminoglycans and proteins. Belongs to the D-glucuronyl C5-epimerase family.

Protein type: EC 5.1.3.17; Isomerase; Glycan Metabolism - heparan sulfate biosynthesis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q23

Cellular Component: Golgi membrane; Golgi apparatus; integral to membrane

Molecular Function: heparosan-N-sulfate-glucuronate 5-epimerase activity; racemase and epimerase activity, acting on carbohydrates and derivatives; UDP-glucuronate 5'-epimerase activity

Biological Process: glycosaminoglycan biosynthetic process; heparin biosynthetic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; heparan sulfate proteoglycan biosynthetic process; pathogenesis
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Research Articles on GLCE

1. GLCE may be used as a potential model to study the functional role of intratumor cell heterogeneity in prostate cancer progression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GLCE sirna

 Reference Product  PubMed Publications
 HS3ST1 sirna  >2 publications with GLCE and HS3ST1
 NDST1 sirna  >2 publications with GLCE and NDST1
 NDST2 sirna  >1 publications with GLCE and NDST2

Pathways associated with GLCE sirna

 Products by Pathway  Pathway Diagram
 Glycosaminoglycan Biosynthesis - Heparan Sulfate / Heparin Pathway antibodies  Glycosaminoglycan Biosynthesis - Heparan Sulfate / Heparin Pathway Diagram
 Glycosaminoglycan Biosynthesis - Heparan Sulfate / Heparin Pathway antibodies  Glycosaminoglycan Biosynthesis - Heparan Sulfate / Heparin Pathway Diagram
 Glycosaminoglycan Biosynthesis, Heparan Sulfate Backbone Pathway antibodies  Glycosaminoglycan Biosynthesis, Heparan Sulfate Backbone Pathway Diagram
 Glycosaminoglycan Biosynthesis, Heparan Sulfate Backbone Pathway antibodies  Glycosaminoglycan Biosynthesis, Heparan Sulfate Backbone Pathway Diagram
 Glycosaminoglycan Metabolism Pathway antibodies  Glycosaminoglycan Metabolism Pathway Diagram
 HS-GAG Biosynthesis Pathway antibodies  HS-GAG Biosynthesis Pathway Diagram
 Heparan Sulfate/heparin (HS-GAG) Metabolism Pathway antibodies  Heparan Sulfate/heparin (HS-GAG) Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Carbohydrates Pathway antibodies  Metabolism Of Carbohydrates Pathway Diagram

Diseases associated with GLCE sirna

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >6 publications with GLCE and Breast Neoplasms
 Hyperplasia Antibodies  >2 publications with GLCE and Hyperplasia
 Inflammation Antibodies  >2 publications with GLCE and Inflammation
 Fibrosis Antibodies  >1 publications with GLCE and Fibrosis
 Necrosis Antibodies  >1 publications with GLCE and Necrosis

Organs/Tissues associated with GLCE sirna

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >4 publications with GLCE and Lung
 Prostate Antibodies  >4 publications with GLCE and Prostate
 Parathyroid Antibodies  >1 publications with GLCE and Parathyroid
 Embryonic Tissue Antibodies  >1 publications with GLCE and Embryonic Tissue
 Bone Antibodies  >1 publications with GLCE and Bone
 Lymph Antibodies  >1 publications with GLCE and Lymph
 Vascular Antibodies  >1 publications with GLCE and Vascular
 Kidney Antibodies  >1 publications with GLCE and Kidney
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