GRIN2C siRNA – Glutamate Receptor Ionotropic, NMDA 2c Isoform 1 (Human)

Name: GRIN2C siRNA; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8221623
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8221623

MBS8221623 is an siRNA targeting GRIN2C. Also known as GRIN2C siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit GRIN2C expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GRIN2C, siRNA

Full Product Name

GRIN2C siRNA (Human)

Product Gene Name

GRIN2C sirna

Product Synonym Names

NMDAR2C; Glutamate receptor ionotropic. NMDA 2C; GluN2C; Glutamate [NMDA] receptor subunit epsilon-3; N-methyl D-aspartate receptor subtype 2C; NMDAR2C; NR2C

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

GRIN2C siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

134,209 Da

OMIM

138254

3D Structure

ModBase 3D Structure for Q14957

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human GRIN2C gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for GRIN2C sirna

RNA Interference (RNAi)

Other Notes

Small volumes of GRIN2C sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GRIN2C. It may not necessarily be applicable to this product.

NCBI GI #

55770854

NCBI GeneID

2905

NCBI Accession #

NP_000826.2

UniProt Primary Accession #

Q14957

UniProt Secondary Accession #

B2RTT1

UniProt Related Accession #

Q14957

GenBank Nucleotide #

NM_000835.4

NCBI Official Full Name

glutamate receptor ionotropic, NMDA 2C isoform 1

NCBI Official Synonym Full Names

glutamate receptor, ionotropic, N-methyl D-aspartate 2C

NCBI Official Symbol

GRIN2C

NCBI Official Synonym Symbols

NR2C; GluN2C; NMDAR2C

UniProt Protein Name

Glutamate receptor ionotropic, NMDA 2C

UniProt Synonym Protein Names

Glutamate [NMDA] receptor subunit epsilon-3; N-methyl D-aspartate receptor subtype 2C; NMDAR2C; NR2C

Protein Family

Glutamate receptor ionotropic

UniProt Gene Name

GRIN2C

UniProt Synonym Gene Names

NMDAR2C; GluN2C; NMDAR2C; NR2C

UniProt Entry Name

NMDE3_HUMAN

NCBI Protein Information

glutamate receptor ionotropic, NMDA 2C

NCBI Summary for GRIN2C

This gene encodes a subunit of the N-methyl-D-aspartate (NMDA) receptor, which is a subtype of ionotropic glutamate receptor. NMDA receptors are found in the central nervous system, are permeable to cations and have an important role in physiological processes such as learning, memory, and synaptic development. The receptor is a tetramer of different subunits (typically heterodimer of subunit 1 with one or more of subunits 2A-D), forming a channel that is permeable to calcium, potassium, and sodium, and whose properties are determined by subunit composition. Alterations in the subunit composition of the receptor are associated with pathophysiological conditions such as Parkinson's disease, Alzheimer's disease, depression, and schizophrenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

UniProt Comments for GRIN2C

NMDAR2C: NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2C/GRIN2C subfamily.

Protein type: Membrane protein, integral; Channel, calcium; Channel, ligand-gated; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: postsynaptic membrane; integral to plasma membrane; dendrite; postsynaptic density; plasma membrane; cell junction; N-methyl-D-aspartate selective glutamate receptor complex

Molecular Function: protein binding; extracellular-glutamate-gated ion channel activity; N-methyl-D-aspartate selective glutamate receptor activity; protein N-terminus binding; cation channel activity; PDZ domain binding

Biological Process: synaptic transmission, glutamatergic; synaptic transmission; protein localization; transport; glutamate signaling pathway; ionotropic glutamate receptor signaling pathway; directional locomotion; response to wounding; regulation of excitatory postsynaptic membrane potential; neuromuscular process controlling balance; negative regulation of protein catabolic process

Research Articles on GRIN2C

1. The major depression subjects exhibited significantly higher expression levels of the NMDA receptor subunit genes GRIN2C.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GRIN2C sirna

Reference Product	PubMed Publications
GRIN1 sirna	>4 publications with GRIN2C and GRIN1

Pathways associated with GRIN2C sirna

Products by Pathway	Pathway Diagram
ARMS-mediated Activation Pathway antibodies	ARMS-mediated Activation Pathway Diagram
Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies	Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Alzheimers Disease Pathway antibodies	Alzheimers Disease Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram

Diseases associated with GRIN2C sirna

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>3 publications with GRIN2C and Nervous System Diseases
Depressive Disorder Antibodies	>3 publications with GRIN2C and Depressive Disorder
Hypertrophy Antibodies	>3 publications with GRIN2C and Hypertrophy
Disease Models, Animal Antibodies	>3 publications with GRIN2C and Disease Models, Animal
Learning Disorders Antibodies	>3 publications with GRIN2C and Learning Disorders
Liver Diseases Antibodies	>2 publications with GRIN2C and Liver Diseases
Hepatomegaly Antibodies	>2 publications with GRIN2C and Hepatomegaly
Necrosis Antibodies	>2 publications with GRIN2C and Necrosis
Brain Diseases Antibodies	>1 publications with GRIN2C and Brain Diseases
Memory Disorders Antibodies	>1 publications with GRIN2C and Memory Disorders

Organs/Tissues associated with GRIN2C sirna

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>13 publications with GRIN2C and Brain