HOXA4 siRNA – Homeobox Protein Hox-A4 (Human)

Name: HOXA4 siRNA; Homeobox Protein Hox-A4; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8239104
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8239104

MBS8239104 is an siRNA targeting HOXA4. Also known as HOXA4 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit HOXA4 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

HOXA4, siRNA

Full Product Name

HOXA4 siRNA (Human)

Product Gene Name

HOXA4 sirna

Product Synonym Names

HOX1D; Homeobox protein Hox-A4; Homeobox protein Hox-1.4; Homeobox protein Hox-1D

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

HOXA4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

34,499 Da

OMIM

142953

3D Structure

ModBase 3D Structure for Q00056

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HOXA4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for HOXA4 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of HOXA4 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HOXA4. It may not necessarily be applicable to this product.

NCBI GI #

148613882

NCBI GeneID

3201

NCBI Accession #

NP_002132.3

UniProt Primary Accession #

Q00056

UniProt Secondary Accession #

O43366; A4D180

UniProt Related Accession #

Q00056

GenBank Nucleotide #

NM_002141.4

NCBI Official Full Name

homeobox protein Hox-A4

NCBI Official Synonym Full Names

homeobox A4

NCBI Official Symbol

HOXA4

NCBI Official Synonym Symbols

HOX1; HOX1D

UniProt Protein Name

Homeobox protein Hox-A4

UniProt Synonym Protein Names

Homeobox protein Hox-1.4; Homeobox protein Hox-1D

Protein Family

Homeobox protein

UniProt Gene Name

HOXA4

UniProt Synonym Gene Names

HOX1D

UniProt Entry Name

HXA4_HUMAN

NCBI Protein Information

homeobox protein Hox-A4

NCBI Summary for HOXA4

In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. [provided by RefSeq, Jul 2008]

UniProt Comments for HOXA4

HOXA4: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to sites in the 5'-flanking sequence of its coding region with various affinities. The consensus sequences of the high and low affinity binding sites are 5'-TAATGA[CG]-3' and 5'-CTAATTTT- 3'. Belongs to the Antp homeobox family. Deformed subfamily.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 7p15.2

Cellular Component: nucleus

Molecular Function: sequence-specific DNA binding; transcription factor activity

Biological Process: anterior/posterior pattern formation; anatomical structure morphogenesis; regulation of transcription, DNA-dependent; transcription, DNA-dependent; embryonic skeletal morphogenesis

Research Articles on HOXA4

1. promoter hypermethylation of HOXA4 gene could be an epigenetic mechanism mediating IM resistance in CML patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with HOXA4 sirna

Reference Product	PubMed Publications
PBX1 sirna	>2 publications with HOXA4 and PBX1
NFE2L1 sirna	>1 publications with HOXA4 and NFE2L1
PBX3 sirna	>1 publications with HOXA4 and PBX3

Diseases associated with HOXA4 sirna

Disease Name	Pubmed Publications
Genital Diseases, Male Antibodies	>13 publications with HOXA4 and Genital Diseases, Male
Congenital Abnormalities Antibodies	>8 publications with HOXA4 and Congenital Abnormalities
Lung Neoplasms Antibodies	>7 publications with HOXA4 and Lung Neoplasms
Hematologic Diseases Antibodies	>7 publications with HOXA4 and Hematologic Diseases
Immune System Diseases Antibodies	>5 publications with HOXA4 and Immune System Diseases
Lung Diseases Antibodies	>5 publications with HOXA4 and Lung Diseases
Breast Neoplasms Antibodies	>3 publications with HOXA4 and Breast Neoplasms
Cell Transformation, Neoplastic Antibodies	>3 publications with HOXA4 and Cell Transformation, Neoplastic
Neoplasms, Experimental Antibodies	>3 publications with HOXA4 and Neoplasms, Experimental
Inflammation Antibodies	>3 publications with HOXA4 and Inflammation

Organs/Tissues associated with HOXA4 sirna

Organ/Tissue Name	Pubmed Publications
Embryonic Tissue Antibodies	>23 publications with HOXA4 and Embryonic Tissue
Bone Antibodies	>21 publications with HOXA4 and Bone
Lung Antibodies	>16 publications with HOXA4 and Lung
Skin Antibodies	>10 publications with HOXA4 and Skin
Brain Antibodies	>10 publications with HOXA4 and Brain
Intestine Antibodies	>7 publications with HOXA4 and Intestine
Connective Tissue Antibodies	>5 publications with HOXA4 and Connective Tissue
Testis Antibodies	>4 publications with HOXA4 and Testis
Stomach Antibodies	>3 publications with HOXA4 and Stomach
Ovary Antibodies	>2 publications with HOXA4 and Ovary