KIFAP3 siRNA – Kinesin-Associated Protein 3 Isoform 2 (Human)

Name: KIFAP3 siRNA; Human; MyBioSource
Brand: MyBioSource
SKU: MBS8204849
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8204849

MBS8204849 is an siRNA targeting KIFAP3. Also known as KIFAP3 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit KIFAP3 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

KIFAP3, siRNA

Full Product Name

KIFAP3 siRNA (Human)

Product Gene Name

KIFAP3 sirna

Product Synonym Names

KIF3AP; SMAP; Kinesin-associated protein 3; KAP-3; KAP3; Smg GDS-associated protein

Host

Synthetic

Species Reactivity

Human

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

KIFAP3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

82,144 Da

OMIM

601836

3D Structure

ModBase 3D Structure for Q92845

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KIFAP3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for KIFAP3 sirna

RNA Interference (RNAi)

Other Notes

Small volumes of KIFAP3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KIFAP3. It may not necessarily be applicable to this product.

NCBI GI #

325197182

NCBI GeneID

22920

NCBI Accession #

NP_001191443.1

UniProt Primary Accession #

Q92845

UniProt Secondary Accession #

Q8NHU7; Q9H416; B1AKU4; B1AKU5; B2RDL1; B7Z8A3; F5H591

UniProt Related Accession #

Q92845

GenBank Nucleotide #

NM_001204514.1

NCBI Official Full Name

kinesin-associated protein 3 isoform 2

NCBI Official Synonym Full Names

kinesin-associated protein 3

NCBI Official Symbol

KIFAP3

NCBI Official Synonym Symbols

FLA3; KAP3; SMAP; KAP-1; KAP-3; Smg-GDS; dJ190I16.1

UniProt Protein Name

Kinesin-associated protein 3

UniProt Synonym Protein Names

Smg GDS-associated protein

Protein Family

Kinesin-associated protein

UniProt Gene Name

KIFAP3

UniProt Synonym Gene Names

KIF3AP; SMAP; KAP-3; KAP3

UniProt Entry Name

KIFA3_HUMAN

NCBI Protein Information

kinesin-associated protein 3

NCBI Summary for KIFAP3

The small G protein GDP dissociation stimulator (smg GDS) is a regulator protein having two activities on a group of small G proteins including the Rho and Rap1 family members and Ki-Ras; one is to stimulate their GDP/GTP exchange reactions, and the other is to inhibit their interactions with membranes. The protein encoded by this gene contains 9 'Armadillo' repeats and interacts with the smg GDS protein through these repeats. This protein, which is highly concentrated around the endoplasmic reticulum, is phosphorylated by v-src, and this phosphorylation reduces the affinity of the protein for smg GDS. It is thought that this protein serves as a linker between human chromosome-associated polypeptide (HCAP) and KIF3A/B, a kinesin superfamily protein in the nucleus, and that it plays a role in the interaction of chromosomes with an ATPase motor protein. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

UniProt Comments for KIFAP3

KIFAP3: Involved in tethering the chromosomes to the spindle pole and in chromosome movement. Binds to the tail domain of the KIF3A/KIF3B heterodimer to form a heterotrimeric KIF3 complex and may regulate the membrane binding of this complex.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q24.2

Cellular Component: microtubule cytoskeleton; Golgi apparatus; centrosome; condensed nuclear chromosome; endoplasmic reticulum; spindle microtubule; kinesin II complex; axoneme; cytosol; photoreceptor connecting cilium

Molecular Function: protein binding; kinesin binding

Biological Process: negative regulation of cell proliferation; protein localization; positive regulation of calcium-dependent cell-cell adhesion; organelle organization and biogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class II; protein complex assembly; signal transduction; blood coagulation; microtubule-based movement; negative regulation of apoptosis; microtubule-based process

Research Articles on KIFAP3

1. Common genetic variation in the Kinesin-Associated Protein 3 (KIFAP3) gene (rs1541160) was reported to have a significant effect on survival in amyotrophic lateral sclerosis patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with KIFAP3 sirna

Reference Product	PubMed Publications
KIF3A sirna	>6 publications with KIFAP3 and KIF3A
KIF3B sirna	>3 publications with KIFAP3 and KIF3B
CHAT sirna	>1 publications with KIFAP3 and CHAT

Pathways associated with KIFAP3 sirna

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Assembly Of The Primary Cilium Pathway antibodies	Assembly Of The Primary Cilium Pathway Diagram
Factors Involved In Megakaryocyte Development And Platelet Production Pathway antibodies	Factors Involved In Megakaryocyte Development And Platelet Production Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Intraflagellar Transport Pathway antibodies	Intraflagellar Transport Pathway Diagram
Kinesins Pathway antibodies	Kinesins Pathway Diagram
MHC Class II Antigen Presentation Pathway antibodies	MHC Class II Antigen Presentation Pathway Diagram
Membrane Trafficking Pathway antibodies	Membrane Trafficking Pathway Diagram
Organelle Biogenesis And Maintenance Pathway antibodies	Organelle Biogenesis And Maintenance Pathway Diagram

Diseases associated with KIFAP3 sirna

Disease Name	Pubmed Publications
Brain Diseases Antibodies	>1 publications with KIFAP3 and Brain Diseases
Hypertrophy Antibodies	>1 publications with KIFAP3 and Hypertrophy
Kidney Diseases Antibodies	>1 publications with KIFAP3 and Kidney Diseases
Fibrosis Antibodies	>1 publications with KIFAP3 and Fibrosis
Necrosis Antibodies	>1 publications with KIFAP3 and Necrosis

Organs/Tissues associated with KIFAP3 sirna

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>6 publications with KIFAP3 and Brain
Blood Antibodies	>2 publications with KIFAP3 and Blood
Testis Antibodies	>2 publications with KIFAP3 and Testis
Spinal Cord Antibodies	>1 publications with KIFAP3 and Spinal Cord
Muscle Antibodies	>1 publications with KIFAP3 and Muscle
Vascular Antibodies	>1 publications with KIFAP3 and Vascular
Kidney Antibodies	>1 publications with KIFAP3 and Kidney